Cargando…

The Antimicrobial Properties of Chitosan Can Be Tailored by Formulation

Topical administration of drugs into the vagina can provide local therapy of vaginal infections, preventing the possible systemic side effects of the drugs. The natural polysaccharide chitosan is known for its excellent mucoadhesive properties, safety profile, and antibacterial effects, and thus it...

Descripción completa

Detalles Bibliográficos
Autores principales: Jøraholmen, May Wenche, Bhargava, Abhilasha, Julin, Kjersti, Johannessen, Mona, Škalko-Basnet, Nataša
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7074233/
https://www.ncbi.nlm.nih.gov/pubmed/32023890
http://dx.doi.org/10.3390/md18020096
_version_ 1783506786617131008
author Jøraholmen, May Wenche
Bhargava, Abhilasha
Julin, Kjersti
Johannessen, Mona
Škalko-Basnet, Nataša
author_facet Jøraholmen, May Wenche
Bhargava, Abhilasha
Julin, Kjersti
Johannessen, Mona
Škalko-Basnet, Nataša
author_sort Jøraholmen, May Wenche
collection PubMed
description Topical administration of drugs into the vagina can provide local therapy of vaginal infections, preventing the possible systemic side effects of the drugs. The natural polysaccharide chitosan is known for its excellent mucoadhesive properties, safety profile, and antibacterial effects, and thus it can be utilized in improving localized vaginal therapy by prolonging the residence time of a drug at the vaginal site while acting as an antimicrobial in synergy. Therefore, we aimed to explore the potential of chitosan, namely chitosan-coated liposomes and chitosan hydrogel, as an excipient with intrinsic antimicrobial properties. Liposomes were prepared by the thin-film hydration method followed by vesicle size reduction by sonication to the desired size, approximately 200 nm, and coated with chitosan (0.01, 0.03, 0.1, and 0.3%, w/v, respectively). The mucoadhesive properties of chitosan-coated liposomes were determined through their binding efficiency to mucin compared to non-coated liposomes. Non-coated liposomal suspensions were incorporated in chitosan hydrogels forming the liposomes-in-hydrogel formulations, which were further assessed for their texture properties in the presence of biological fluid simulants. The antibacterial effect of chitosan-coated liposomes (0.03%, 0.1% and 0.3%, w/v) and chitosan hydrogels (0.1% and 0.3%, w/w) on Staphylococcus epidermidis and Staphylococcus aureus was successfully confirmed.
format Online
Article
Text
id pubmed-7074233
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-70742332020-03-19 The Antimicrobial Properties of Chitosan Can Be Tailored by Formulation Jøraholmen, May Wenche Bhargava, Abhilasha Julin, Kjersti Johannessen, Mona Škalko-Basnet, Nataša Mar Drugs Article Topical administration of drugs into the vagina can provide local therapy of vaginal infections, preventing the possible systemic side effects of the drugs. The natural polysaccharide chitosan is known for its excellent mucoadhesive properties, safety profile, and antibacterial effects, and thus it can be utilized in improving localized vaginal therapy by prolonging the residence time of a drug at the vaginal site while acting as an antimicrobial in synergy. Therefore, we aimed to explore the potential of chitosan, namely chitosan-coated liposomes and chitosan hydrogel, as an excipient with intrinsic antimicrobial properties. Liposomes were prepared by the thin-film hydration method followed by vesicle size reduction by sonication to the desired size, approximately 200 nm, and coated with chitosan (0.01, 0.03, 0.1, and 0.3%, w/v, respectively). The mucoadhesive properties of chitosan-coated liposomes were determined through their binding efficiency to mucin compared to non-coated liposomes. Non-coated liposomal suspensions were incorporated in chitosan hydrogels forming the liposomes-in-hydrogel formulations, which were further assessed for their texture properties in the presence of biological fluid simulants. The antibacterial effect of chitosan-coated liposomes (0.03%, 0.1% and 0.3%, w/v) and chitosan hydrogels (0.1% and 0.3%, w/w) on Staphylococcus epidermidis and Staphylococcus aureus was successfully confirmed. MDPI 2020-01-31 /pmc/articles/PMC7074233/ /pubmed/32023890 http://dx.doi.org/10.3390/md18020096 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jøraholmen, May Wenche
Bhargava, Abhilasha
Julin, Kjersti
Johannessen, Mona
Škalko-Basnet, Nataša
The Antimicrobial Properties of Chitosan Can Be Tailored by Formulation
title The Antimicrobial Properties of Chitosan Can Be Tailored by Formulation
title_full The Antimicrobial Properties of Chitosan Can Be Tailored by Formulation
title_fullStr The Antimicrobial Properties of Chitosan Can Be Tailored by Formulation
title_full_unstemmed The Antimicrobial Properties of Chitosan Can Be Tailored by Formulation
title_short The Antimicrobial Properties of Chitosan Can Be Tailored by Formulation
title_sort antimicrobial properties of chitosan can be tailored by formulation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7074233/
https://www.ncbi.nlm.nih.gov/pubmed/32023890
http://dx.doi.org/10.3390/md18020096
work_keys_str_mv AT jøraholmenmaywenche theantimicrobialpropertiesofchitosancanbetailoredbyformulation
AT bhargavaabhilasha theantimicrobialpropertiesofchitosancanbetailoredbyformulation
AT julinkjersti theantimicrobialpropertiesofchitosancanbetailoredbyformulation
AT johannessenmona theantimicrobialpropertiesofchitosancanbetailoredbyformulation
AT skalkobasnetnatasa theantimicrobialpropertiesofchitosancanbetailoredbyformulation
AT jøraholmenmaywenche antimicrobialpropertiesofchitosancanbetailoredbyformulation
AT bhargavaabhilasha antimicrobialpropertiesofchitosancanbetailoredbyformulation
AT julinkjersti antimicrobialpropertiesofchitosancanbetailoredbyformulation
AT johannessenmona antimicrobialpropertiesofchitosancanbetailoredbyformulation
AT skalkobasnetnatasa antimicrobialpropertiesofchitosancanbetailoredbyformulation