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The Antimicrobial Properties of Chitosan Can Be Tailored by Formulation
Topical administration of drugs into the vagina can provide local therapy of vaginal infections, preventing the possible systemic side effects of the drugs. The natural polysaccharide chitosan is known for its excellent mucoadhesive properties, safety profile, and antibacterial effects, and thus it...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7074233/ https://www.ncbi.nlm.nih.gov/pubmed/32023890 http://dx.doi.org/10.3390/md18020096 |
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author | Jøraholmen, May Wenche Bhargava, Abhilasha Julin, Kjersti Johannessen, Mona Škalko-Basnet, Nataša |
author_facet | Jøraholmen, May Wenche Bhargava, Abhilasha Julin, Kjersti Johannessen, Mona Škalko-Basnet, Nataša |
author_sort | Jøraholmen, May Wenche |
collection | PubMed |
description | Topical administration of drugs into the vagina can provide local therapy of vaginal infections, preventing the possible systemic side effects of the drugs. The natural polysaccharide chitosan is known for its excellent mucoadhesive properties, safety profile, and antibacterial effects, and thus it can be utilized in improving localized vaginal therapy by prolonging the residence time of a drug at the vaginal site while acting as an antimicrobial in synergy. Therefore, we aimed to explore the potential of chitosan, namely chitosan-coated liposomes and chitosan hydrogel, as an excipient with intrinsic antimicrobial properties. Liposomes were prepared by the thin-film hydration method followed by vesicle size reduction by sonication to the desired size, approximately 200 nm, and coated with chitosan (0.01, 0.03, 0.1, and 0.3%, w/v, respectively). The mucoadhesive properties of chitosan-coated liposomes were determined through their binding efficiency to mucin compared to non-coated liposomes. Non-coated liposomal suspensions were incorporated in chitosan hydrogels forming the liposomes-in-hydrogel formulations, which were further assessed for their texture properties in the presence of biological fluid simulants. The antibacterial effect of chitosan-coated liposomes (0.03%, 0.1% and 0.3%, w/v) and chitosan hydrogels (0.1% and 0.3%, w/w) on Staphylococcus epidermidis and Staphylococcus aureus was successfully confirmed. |
format | Online Article Text |
id | pubmed-7074233 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-70742332020-03-19 The Antimicrobial Properties of Chitosan Can Be Tailored by Formulation Jøraholmen, May Wenche Bhargava, Abhilasha Julin, Kjersti Johannessen, Mona Škalko-Basnet, Nataša Mar Drugs Article Topical administration of drugs into the vagina can provide local therapy of vaginal infections, preventing the possible systemic side effects of the drugs. The natural polysaccharide chitosan is known for its excellent mucoadhesive properties, safety profile, and antibacterial effects, and thus it can be utilized in improving localized vaginal therapy by prolonging the residence time of a drug at the vaginal site while acting as an antimicrobial in synergy. Therefore, we aimed to explore the potential of chitosan, namely chitosan-coated liposomes and chitosan hydrogel, as an excipient with intrinsic antimicrobial properties. Liposomes were prepared by the thin-film hydration method followed by vesicle size reduction by sonication to the desired size, approximately 200 nm, and coated with chitosan (0.01, 0.03, 0.1, and 0.3%, w/v, respectively). The mucoadhesive properties of chitosan-coated liposomes were determined through their binding efficiency to mucin compared to non-coated liposomes. Non-coated liposomal suspensions were incorporated in chitosan hydrogels forming the liposomes-in-hydrogel formulations, which were further assessed for their texture properties in the presence of biological fluid simulants. The antibacterial effect of chitosan-coated liposomes (0.03%, 0.1% and 0.3%, w/v) and chitosan hydrogels (0.1% and 0.3%, w/w) on Staphylococcus epidermidis and Staphylococcus aureus was successfully confirmed. MDPI 2020-01-31 /pmc/articles/PMC7074233/ /pubmed/32023890 http://dx.doi.org/10.3390/md18020096 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Jøraholmen, May Wenche Bhargava, Abhilasha Julin, Kjersti Johannessen, Mona Škalko-Basnet, Nataša The Antimicrobial Properties of Chitosan Can Be Tailored by Formulation |
title | The Antimicrobial Properties of Chitosan Can Be Tailored by Formulation |
title_full | The Antimicrobial Properties of Chitosan Can Be Tailored by Formulation |
title_fullStr | The Antimicrobial Properties of Chitosan Can Be Tailored by Formulation |
title_full_unstemmed | The Antimicrobial Properties of Chitosan Can Be Tailored by Formulation |
title_short | The Antimicrobial Properties of Chitosan Can Be Tailored by Formulation |
title_sort | antimicrobial properties of chitosan can be tailored by formulation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7074233/ https://www.ncbi.nlm.nih.gov/pubmed/32023890 http://dx.doi.org/10.3390/md18020096 |
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