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Differential behavior of NF-κB, IκBα and EGFR during the renal carcinogenic process in an experimental model in vivo
Renal cell carcinoma (RCC) is the most common type of cancer of the adult kidney. It is generally asymptomatic even at advanced stages, so opportune diagnosis is rare, making it almost impossible to study this cancer at its early stages. RCC tumors induced by ferric nitrilotriacetate (FeNTA) in rats...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7074249/ https://www.ncbi.nlm.nih.gov/pubmed/32256811 http://dx.doi.org/10.3892/ol.2020.11436 |
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author | Pariente-Pérez, Telma Aguilar-Alonso, Francisco Solano, José Dolores Vargas-Olvera, Chabetty Curiel-Muñiz, Patricia Mendoza-Rodríguez, Carmen Adriana Tenorio-Hernández, Daniela Ibarra-Rubio, María Elena |
author_facet | Pariente-Pérez, Telma Aguilar-Alonso, Francisco Solano, José Dolores Vargas-Olvera, Chabetty Curiel-Muñiz, Patricia Mendoza-Rodríguez, Carmen Adriana Tenorio-Hernández, Daniela Ibarra-Rubio, María Elena |
author_sort | Pariente-Pérez, Telma |
collection | PubMed |
description | Renal cell carcinoma (RCC) is the most common type of cancer of the adult kidney. It is generally asymptomatic even at advanced stages, so opportune diagnosis is rare, making it almost impossible to study this cancer at its early stages. RCC tumors induced by ferric nitrilotriacetate (FeNTA) in rats histologically correspond to the human clear cell RCC subtype (ccRCC) and the exposure to this carcinogen during either one or two months leads to different early stages of neoplastic development. High levels of nuclear factor kappa B (NF-κB) and epidermal growth factor receptor (EGFR) as well as low levels of NF-κB inhibitor alpha (IκBα) are frequent in human RCC, but their status in FeNTA-induced tumors and their evolution along renal carcinogenesis is unclear. On this basis, in the present study NF-κB, IκBα and EGFR behavior was analyzed at different stages of the experimental renal carcinogenesis model. Similar to patients with RCC, neoplastic tissue showed high levels of p65, one of the predominant subunits of NF-κB in ccRCC and of EGFR (protein and mRNA), as well as a decrease in the levels of NF-κB's main inhibitor, IκBα, resulting in a classic oncogenic combination. Conversely, different responses were observed at early stages of carcinogenesis. After one month of FeNTA-exposure, NF-κB activity and EGFR levels augmented; but unexpectedly, IκBα also did. While after two months, NF-κB activity diminished, but EGFR and IκBα levels remained elevated. In conclusion, FeNTA-induced tumors and RCC human neoplasms are analogues regarding to the classic NF-κB, IκBα and EGFR behavior, and distinctive non-conventional combination of changes is developed at each early stage studied. The results obtained suggest that the dysregulation of the analyzed molecules could be related to different signaling pathways and therefore, to particular effects depending on the phase of the carcinogenic process. |
format | Online Article Text |
id | pubmed-7074249 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-70742492020-03-31 Differential behavior of NF-κB, IκBα and EGFR during the renal carcinogenic process in an experimental model in vivo Pariente-Pérez, Telma Aguilar-Alonso, Francisco Solano, José Dolores Vargas-Olvera, Chabetty Curiel-Muñiz, Patricia Mendoza-Rodríguez, Carmen Adriana Tenorio-Hernández, Daniela Ibarra-Rubio, María Elena Oncol Lett Articles Renal cell carcinoma (RCC) is the most common type of cancer of the adult kidney. It is generally asymptomatic even at advanced stages, so opportune diagnosis is rare, making it almost impossible to study this cancer at its early stages. RCC tumors induced by ferric nitrilotriacetate (FeNTA) in rats histologically correspond to the human clear cell RCC subtype (ccRCC) and the exposure to this carcinogen during either one or two months leads to different early stages of neoplastic development. High levels of nuclear factor kappa B (NF-κB) and epidermal growth factor receptor (EGFR) as well as low levels of NF-κB inhibitor alpha (IκBα) are frequent in human RCC, but their status in FeNTA-induced tumors and their evolution along renal carcinogenesis is unclear. On this basis, in the present study NF-κB, IκBα and EGFR behavior was analyzed at different stages of the experimental renal carcinogenesis model. Similar to patients with RCC, neoplastic tissue showed high levels of p65, one of the predominant subunits of NF-κB in ccRCC and of EGFR (protein and mRNA), as well as a decrease in the levels of NF-κB's main inhibitor, IκBα, resulting in a classic oncogenic combination. Conversely, different responses were observed at early stages of carcinogenesis. After one month of FeNTA-exposure, NF-κB activity and EGFR levels augmented; but unexpectedly, IκBα also did. While after two months, NF-κB activity diminished, but EGFR and IκBα levels remained elevated. In conclusion, FeNTA-induced tumors and RCC human neoplasms are analogues regarding to the classic NF-κB, IκBα and EGFR behavior, and distinctive non-conventional combination of changes is developed at each early stage studied. The results obtained suggest that the dysregulation of the analyzed molecules could be related to different signaling pathways and therefore, to particular effects depending on the phase of the carcinogenic process. D.A. Spandidos 2020-04 2020-03-03 /pmc/articles/PMC7074249/ /pubmed/32256811 http://dx.doi.org/10.3892/ol.2020.11436 Text en Copyright: © Pariente-Pérez et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Pariente-Pérez, Telma Aguilar-Alonso, Francisco Solano, José Dolores Vargas-Olvera, Chabetty Curiel-Muñiz, Patricia Mendoza-Rodríguez, Carmen Adriana Tenorio-Hernández, Daniela Ibarra-Rubio, María Elena Differential behavior of NF-κB, IκBα and EGFR during the renal carcinogenic process in an experimental model in vivo |
title | Differential behavior of NF-κB, IκBα and EGFR during the renal carcinogenic process in an experimental model in vivo |
title_full | Differential behavior of NF-κB, IκBα and EGFR during the renal carcinogenic process in an experimental model in vivo |
title_fullStr | Differential behavior of NF-κB, IκBα and EGFR during the renal carcinogenic process in an experimental model in vivo |
title_full_unstemmed | Differential behavior of NF-κB, IκBα and EGFR during the renal carcinogenic process in an experimental model in vivo |
title_short | Differential behavior of NF-κB, IκBα and EGFR during the renal carcinogenic process in an experimental model in vivo |
title_sort | differential behavior of nf-κb, iκbα and egfr during the renal carcinogenic process in an experimental model in vivo |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7074249/ https://www.ncbi.nlm.nih.gov/pubmed/32256811 http://dx.doi.org/10.3892/ol.2020.11436 |
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