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Seminal Fluid Metabolomic Markers of Oligozoospermic Infertility in Humans

Infertility affects 12–15% of couples worldwide, and male factors are the cause of nearly half of all cases. Studying seminal fluid composition could lead to additional diagnostic accuracy and a better understanding of the pathophysiology of male factor infertility. Metabolomics offers a new opportu...

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Autores principales: Murgia, Federica, Corda, Valentina, Serrenti, Marianna, Usai, Valeria, Santoru, Maria Laura, Hurt, K. Joseph, Passaretti, Mauro, Monni, Maria Carla, Atzori, Luigi, Monni, Giovanni
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7074256/
https://www.ncbi.nlm.nih.gov/pubmed/32053951
http://dx.doi.org/10.3390/metabo10020064
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author Murgia, Federica
Corda, Valentina
Serrenti, Marianna
Usai, Valeria
Santoru, Maria Laura
Hurt, K. Joseph
Passaretti, Mauro
Monni, Maria Carla
Atzori, Luigi
Monni, Giovanni
author_facet Murgia, Federica
Corda, Valentina
Serrenti, Marianna
Usai, Valeria
Santoru, Maria Laura
Hurt, K. Joseph
Passaretti, Mauro
Monni, Maria Carla
Atzori, Luigi
Monni, Giovanni
author_sort Murgia, Federica
collection PubMed
description Infertility affects 12–15% of couples worldwide, and male factors are the cause of nearly half of all cases. Studying seminal fluid composition could lead to additional diagnostic accuracy and a better understanding of the pathophysiology of male factor infertility. Metabolomics offers a new opportunity to evaluate biomarkers and better understand pathological mechanisms. The aim of the study was to identify new markers or therapeutic targets to improve outcomes in male factor or idiopathic infertility patients. Semen samples were obtained from 29 men with a normal spermogram test, and from 18 oligozoospermic men. Samples were processed and analyzed by Nuclear Magnetic Resonance spectroscopy and, subsequently, multivariate and univariate statistical analyses. Receiving Operator Curves (ROC) and Spearman correlations were also performed. An Orthogonal Partial Least Square Discriminant Analysis supervised multivariate model was devised to compare the groups. The levels of fructose, myo-inositol, aspartate and choline were altered. Moreover, Spearman Correlation associated fructose, aspartate and myo-inositol with the total amount of spermatozoa, total motile spermatozoa, % of immotility and % of “in situ” spermatozoic motility respectively. NMR-based metabolomics allowed the identification of a specific metabolic fingerprint of the seminal fluids of patients affected by oligozoospermia.
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spelling pubmed-70742562020-03-19 Seminal Fluid Metabolomic Markers of Oligozoospermic Infertility in Humans Murgia, Federica Corda, Valentina Serrenti, Marianna Usai, Valeria Santoru, Maria Laura Hurt, K. Joseph Passaretti, Mauro Monni, Maria Carla Atzori, Luigi Monni, Giovanni Metabolites Article Infertility affects 12–15% of couples worldwide, and male factors are the cause of nearly half of all cases. Studying seminal fluid composition could lead to additional diagnostic accuracy and a better understanding of the pathophysiology of male factor infertility. Metabolomics offers a new opportunity to evaluate biomarkers and better understand pathological mechanisms. The aim of the study was to identify new markers or therapeutic targets to improve outcomes in male factor or idiopathic infertility patients. Semen samples were obtained from 29 men with a normal spermogram test, and from 18 oligozoospermic men. Samples were processed and analyzed by Nuclear Magnetic Resonance spectroscopy and, subsequently, multivariate and univariate statistical analyses. Receiving Operator Curves (ROC) and Spearman correlations were also performed. An Orthogonal Partial Least Square Discriminant Analysis supervised multivariate model was devised to compare the groups. The levels of fructose, myo-inositol, aspartate and choline were altered. Moreover, Spearman Correlation associated fructose, aspartate and myo-inositol with the total amount of spermatozoa, total motile spermatozoa, % of immotility and % of “in situ” spermatozoic motility respectively. NMR-based metabolomics allowed the identification of a specific metabolic fingerprint of the seminal fluids of patients affected by oligozoospermia. MDPI 2020-02-11 /pmc/articles/PMC7074256/ /pubmed/32053951 http://dx.doi.org/10.3390/metabo10020064 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Murgia, Federica
Corda, Valentina
Serrenti, Marianna
Usai, Valeria
Santoru, Maria Laura
Hurt, K. Joseph
Passaretti, Mauro
Monni, Maria Carla
Atzori, Luigi
Monni, Giovanni
Seminal Fluid Metabolomic Markers of Oligozoospermic Infertility in Humans
title Seminal Fluid Metabolomic Markers of Oligozoospermic Infertility in Humans
title_full Seminal Fluid Metabolomic Markers of Oligozoospermic Infertility in Humans
title_fullStr Seminal Fluid Metabolomic Markers of Oligozoospermic Infertility in Humans
title_full_unstemmed Seminal Fluid Metabolomic Markers of Oligozoospermic Infertility in Humans
title_short Seminal Fluid Metabolomic Markers of Oligozoospermic Infertility in Humans
title_sort seminal fluid metabolomic markers of oligozoospermic infertility in humans
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7074256/
https://www.ncbi.nlm.nih.gov/pubmed/32053951
http://dx.doi.org/10.3390/metabo10020064
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