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Seminal Fluid Metabolomic Markers of Oligozoospermic Infertility in Humans
Infertility affects 12–15% of couples worldwide, and male factors are the cause of nearly half of all cases. Studying seminal fluid composition could lead to additional diagnostic accuracy and a better understanding of the pathophysiology of male factor infertility. Metabolomics offers a new opportu...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7074256/ https://www.ncbi.nlm.nih.gov/pubmed/32053951 http://dx.doi.org/10.3390/metabo10020064 |
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author | Murgia, Federica Corda, Valentina Serrenti, Marianna Usai, Valeria Santoru, Maria Laura Hurt, K. Joseph Passaretti, Mauro Monni, Maria Carla Atzori, Luigi Monni, Giovanni |
author_facet | Murgia, Federica Corda, Valentina Serrenti, Marianna Usai, Valeria Santoru, Maria Laura Hurt, K. Joseph Passaretti, Mauro Monni, Maria Carla Atzori, Luigi Monni, Giovanni |
author_sort | Murgia, Federica |
collection | PubMed |
description | Infertility affects 12–15% of couples worldwide, and male factors are the cause of nearly half of all cases. Studying seminal fluid composition could lead to additional diagnostic accuracy and a better understanding of the pathophysiology of male factor infertility. Metabolomics offers a new opportunity to evaluate biomarkers and better understand pathological mechanisms. The aim of the study was to identify new markers or therapeutic targets to improve outcomes in male factor or idiopathic infertility patients. Semen samples were obtained from 29 men with a normal spermogram test, and from 18 oligozoospermic men. Samples were processed and analyzed by Nuclear Magnetic Resonance spectroscopy and, subsequently, multivariate and univariate statistical analyses. Receiving Operator Curves (ROC) and Spearman correlations were also performed. An Orthogonal Partial Least Square Discriminant Analysis supervised multivariate model was devised to compare the groups. The levels of fructose, myo-inositol, aspartate and choline were altered. Moreover, Spearman Correlation associated fructose, aspartate and myo-inositol with the total amount of spermatozoa, total motile spermatozoa, % of immotility and % of “in situ” spermatozoic motility respectively. NMR-based metabolomics allowed the identification of a specific metabolic fingerprint of the seminal fluids of patients affected by oligozoospermia. |
format | Online Article Text |
id | pubmed-7074256 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-70742562020-03-19 Seminal Fluid Metabolomic Markers of Oligozoospermic Infertility in Humans Murgia, Federica Corda, Valentina Serrenti, Marianna Usai, Valeria Santoru, Maria Laura Hurt, K. Joseph Passaretti, Mauro Monni, Maria Carla Atzori, Luigi Monni, Giovanni Metabolites Article Infertility affects 12–15% of couples worldwide, and male factors are the cause of nearly half of all cases. Studying seminal fluid composition could lead to additional diagnostic accuracy and a better understanding of the pathophysiology of male factor infertility. Metabolomics offers a new opportunity to evaluate biomarkers and better understand pathological mechanisms. The aim of the study was to identify new markers or therapeutic targets to improve outcomes in male factor or idiopathic infertility patients. Semen samples were obtained from 29 men with a normal spermogram test, and from 18 oligozoospermic men. Samples were processed and analyzed by Nuclear Magnetic Resonance spectroscopy and, subsequently, multivariate and univariate statistical analyses. Receiving Operator Curves (ROC) and Spearman correlations were also performed. An Orthogonal Partial Least Square Discriminant Analysis supervised multivariate model was devised to compare the groups. The levels of fructose, myo-inositol, aspartate and choline were altered. Moreover, Spearman Correlation associated fructose, aspartate and myo-inositol with the total amount of spermatozoa, total motile spermatozoa, % of immotility and % of “in situ” spermatozoic motility respectively. NMR-based metabolomics allowed the identification of a specific metabolic fingerprint of the seminal fluids of patients affected by oligozoospermia. MDPI 2020-02-11 /pmc/articles/PMC7074256/ /pubmed/32053951 http://dx.doi.org/10.3390/metabo10020064 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Murgia, Federica Corda, Valentina Serrenti, Marianna Usai, Valeria Santoru, Maria Laura Hurt, K. Joseph Passaretti, Mauro Monni, Maria Carla Atzori, Luigi Monni, Giovanni Seminal Fluid Metabolomic Markers of Oligozoospermic Infertility in Humans |
title | Seminal Fluid Metabolomic Markers of Oligozoospermic Infertility in Humans |
title_full | Seminal Fluid Metabolomic Markers of Oligozoospermic Infertility in Humans |
title_fullStr | Seminal Fluid Metabolomic Markers of Oligozoospermic Infertility in Humans |
title_full_unstemmed | Seminal Fluid Metabolomic Markers of Oligozoospermic Infertility in Humans |
title_short | Seminal Fluid Metabolomic Markers of Oligozoospermic Infertility in Humans |
title_sort | seminal fluid metabolomic markers of oligozoospermic infertility in humans |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7074256/ https://www.ncbi.nlm.nih.gov/pubmed/32053951 http://dx.doi.org/10.3390/metabo10020064 |
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