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CRb and PbBa8.1 Synergically Increases Resistant Genes Expression upon Infection of Plasmodiophora brassicae in Brassica napus

PbBa8.1 and CRb are two clubroot-resistant genes that are important for canola breeding in China. Previously, we combined these resistant genes and developed a pyramid-based, homozygous recurrent inbred line (618R), the results of which showed strong resistance to Plasmodiophora brassicae field isol...

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Autores principales: Shah, Nadil, Li, Qian, Xu, Qiang, Liu, Ju, Huang, Fan, Zhan, Zongxiang, Qin, Ping, Zhou, Xueqing, Yu, Wenlin, Zhu, Li, Zhang, Chunyu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7074261/
https://www.ncbi.nlm.nih.gov/pubmed/32079196
http://dx.doi.org/10.3390/genes11020202
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author Shah, Nadil
Li, Qian
Xu, Qiang
Liu, Ju
Huang, Fan
Zhan, Zongxiang
Qin, Ping
Zhou, Xueqing
Yu, Wenlin
Zhu, Li
Zhang, Chunyu
author_facet Shah, Nadil
Li, Qian
Xu, Qiang
Liu, Ju
Huang, Fan
Zhan, Zongxiang
Qin, Ping
Zhou, Xueqing
Yu, Wenlin
Zhu, Li
Zhang, Chunyu
author_sort Shah, Nadil
collection PubMed
description PbBa8.1 and CRb are two clubroot-resistant genes that are important for canola breeding in China. Previously, we combined these resistant genes and developed a pyramid-based, homozygous recurrent inbred line (618R), the results of which showed strong resistance to Plasmodiophora brassicae field isolates; however, the genetic mechanisms of resistance were unclear. In the present work, we conducted comparative RNA sequencing (RNA-Seq) analysis between 618R and its parental lines (305R and 409R) in order to uncover the transcriptomic response of the superior defense mechanisms of 618R and to determine how these two different resistant genes coordinate with each other. Here, we elucidated that the number and expression of differentially expressed genes (DEGs) in 618R are significantly higher than in the parental lines, and PbBa8.1 shares more DEGs and plays a dominant role in the pyramided line. The common DEGs among the lines largely exhibit non-additive expression patterns and enrichment in resistance pathways. Among the enriched pathways, plant–pathogen interaction, plant hormone signaling transduction, and secondary metabolites are the key observation. However, the expressions of the salicylic acid (SA) signaling pathway and reactive oxygen species (ROS) appear to be crucial regulatory components in defense response. Our findings provide comprehensive transcriptomic insight into understanding the interactions of resistance gene pyramids in single lines and can facilitate the breeding of improved resistance in Brassica napus.
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spelling pubmed-70742612020-03-19 CRb and PbBa8.1 Synergically Increases Resistant Genes Expression upon Infection of Plasmodiophora brassicae in Brassica napus Shah, Nadil Li, Qian Xu, Qiang Liu, Ju Huang, Fan Zhan, Zongxiang Qin, Ping Zhou, Xueqing Yu, Wenlin Zhu, Li Zhang, Chunyu Genes (Basel) Article PbBa8.1 and CRb are two clubroot-resistant genes that are important for canola breeding in China. Previously, we combined these resistant genes and developed a pyramid-based, homozygous recurrent inbred line (618R), the results of which showed strong resistance to Plasmodiophora brassicae field isolates; however, the genetic mechanisms of resistance were unclear. In the present work, we conducted comparative RNA sequencing (RNA-Seq) analysis between 618R and its parental lines (305R and 409R) in order to uncover the transcriptomic response of the superior defense mechanisms of 618R and to determine how these two different resistant genes coordinate with each other. Here, we elucidated that the number and expression of differentially expressed genes (DEGs) in 618R are significantly higher than in the parental lines, and PbBa8.1 shares more DEGs and plays a dominant role in the pyramided line. The common DEGs among the lines largely exhibit non-additive expression patterns and enrichment in resistance pathways. Among the enriched pathways, plant–pathogen interaction, plant hormone signaling transduction, and secondary metabolites are the key observation. However, the expressions of the salicylic acid (SA) signaling pathway and reactive oxygen species (ROS) appear to be crucial regulatory components in defense response. Our findings provide comprehensive transcriptomic insight into understanding the interactions of resistance gene pyramids in single lines and can facilitate the breeding of improved resistance in Brassica napus. MDPI 2020-02-17 /pmc/articles/PMC7074261/ /pubmed/32079196 http://dx.doi.org/10.3390/genes11020202 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Shah, Nadil
Li, Qian
Xu, Qiang
Liu, Ju
Huang, Fan
Zhan, Zongxiang
Qin, Ping
Zhou, Xueqing
Yu, Wenlin
Zhu, Li
Zhang, Chunyu
CRb and PbBa8.1 Synergically Increases Resistant Genes Expression upon Infection of Plasmodiophora brassicae in Brassica napus
title CRb and PbBa8.1 Synergically Increases Resistant Genes Expression upon Infection of Plasmodiophora brassicae in Brassica napus
title_full CRb and PbBa8.1 Synergically Increases Resistant Genes Expression upon Infection of Plasmodiophora brassicae in Brassica napus
title_fullStr CRb and PbBa8.1 Synergically Increases Resistant Genes Expression upon Infection of Plasmodiophora brassicae in Brassica napus
title_full_unstemmed CRb and PbBa8.1 Synergically Increases Resistant Genes Expression upon Infection of Plasmodiophora brassicae in Brassica napus
title_short CRb and PbBa8.1 Synergically Increases Resistant Genes Expression upon Infection of Plasmodiophora brassicae in Brassica napus
title_sort crb and pbba8.1 synergically increases resistant genes expression upon infection of plasmodiophora brassicae in brassica napus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7074261/
https://www.ncbi.nlm.nih.gov/pubmed/32079196
http://dx.doi.org/10.3390/genes11020202
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