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3D Chitin Scaffolds of Marine Demosponge Origin for Biomimetic Mollusk Hemolymph-Associated Biomineralization Ex-Vivo
Structure-based tissue engineering requires large-scale 3D cell/tissue manufacture technologies, to produce biologically active scaffolds. Special attention is currently paid to naturally pre-designed scaffolds found in skeletons of marine sponges, which represent a renewable resource of biomaterial...
Autores principales: | , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7074400/ https://www.ncbi.nlm.nih.gov/pubmed/32092907 http://dx.doi.org/10.3390/md18020123 |
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author | Wysokowski, Marcin Machałowski, Tomasz Petrenko, Iaroslav Schimpf, Christian Rafaja, David Galli, Roberta Ziętek, Jerzy Pantović, Snežana Voronkina, Alona Kovalchuk, Valentine Ivanenko, Viatcheslav N. Hoeksema, Bert W. Diaz, Cristina Khrunyk, Yuliya Stelling, Allison L. Giovine, Marco Jesionowski, Teofil Ehrlich, Hermann |
author_facet | Wysokowski, Marcin Machałowski, Tomasz Petrenko, Iaroslav Schimpf, Christian Rafaja, David Galli, Roberta Ziętek, Jerzy Pantović, Snežana Voronkina, Alona Kovalchuk, Valentine Ivanenko, Viatcheslav N. Hoeksema, Bert W. Diaz, Cristina Khrunyk, Yuliya Stelling, Allison L. Giovine, Marco Jesionowski, Teofil Ehrlich, Hermann |
author_sort | Wysokowski, Marcin |
collection | PubMed |
description | Structure-based tissue engineering requires large-scale 3D cell/tissue manufacture technologies, to produce biologically active scaffolds. Special attention is currently paid to naturally pre-designed scaffolds found in skeletons of marine sponges, which represent a renewable resource of biomaterials. Here, an innovative approach to the production of mineralized scaffolds of natural origin is proposed. For the first time, a method to obtain calcium carbonate deposition ex vivo, using living mollusks hemolymph and a marine-sponge-derived template, is specifically described. For this purpose, the marine sponge Aplysin aarcheri and the terrestrial snail Cornu aspersum were selected as appropriate 3D chitinous scaffold and as hemolymph donor, respectively. The formation of calcium-based phase on the surface of chitinous matrix after its immersion into hemolymph was confirmed by Alizarin Red staining. A direct role of mollusks hemocytes is proposed in the creation of fine-tuned microenvironment necessary for calcification ex vivo. The X-ray diffraction pattern of the sample showed a high CaCO(3) amorphous content. Raman spectroscopy evidenced also a crystalline component, with spectra corresponding to biogenic calcite. This study resulted in the development of a new biomimetic product based on ex vivo synthetized ACC and calcite tightly bound to the surface of 3D sponge chitin structure. |
format | Online Article Text |
id | pubmed-7074400 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-70744002020-03-20 3D Chitin Scaffolds of Marine Demosponge Origin for Biomimetic Mollusk Hemolymph-Associated Biomineralization Ex-Vivo Wysokowski, Marcin Machałowski, Tomasz Petrenko, Iaroslav Schimpf, Christian Rafaja, David Galli, Roberta Ziętek, Jerzy Pantović, Snežana Voronkina, Alona Kovalchuk, Valentine Ivanenko, Viatcheslav N. Hoeksema, Bert W. Diaz, Cristina Khrunyk, Yuliya Stelling, Allison L. Giovine, Marco Jesionowski, Teofil Ehrlich, Hermann Mar Drugs Article Structure-based tissue engineering requires large-scale 3D cell/tissue manufacture technologies, to produce biologically active scaffolds. Special attention is currently paid to naturally pre-designed scaffolds found in skeletons of marine sponges, which represent a renewable resource of biomaterials. Here, an innovative approach to the production of mineralized scaffolds of natural origin is proposed. For the first time, a method to obtain calcium carbonate deposition ex vivo, using living mollusks hemolymph and a marine-sponge-derived template, is specifically described. For this purpose, the marine sponge Aplysin aarcheri and the terrestrial snail Cornu aspersum were selected as appropriate 3D chitinous scaffold and as hemolymph donor, respectively. The formation of calcium-based phase on the surface of chitinous matrix after its immersion into hemolymph was confirmed by Alizarin Red staining. A direct role of mollusks hemocytes is proposed in the creation of fine-tuned microenvironment necessary for calcification ex vivo. The X-ray diffraction pattern of the sample showed a high CaCO(3) amorphous content. Raman spectroscopy evidenced also a crystalline component, with spectra corresponding to biogenic calcite. This study resulted in the development of a new biomimetic product based on ex vivo synthetized ACC and calcite tightly bound to the surface of 3D sponge chitin structure. MDPI 2020-02-19 /pmc/articles/PMC7074400/ /pubmed/32092907 http://dx.doi.org/10.3390/md18020123 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Wysokowski, Marcin Machałowski, Tomasz Petrenko, Iaroslav Schimpf, Christian Rafaja, David Galli, Roberta Ziętek, Jerzy Pantović, Snežana Voronkina, Alona Kovalchuk, Valentine Ivanenko, Viatcheslav N. Hoeksema, Bert W. Diaz, Cristina Khrunyk, Yuliya Stelling, Allison L. Giovine, Marco Jesionowski, Teofil Ehrlich, Hermann 3D Chitin Scaffolds of Marine Demosponge Origin for Biomimetic Mollusk Hemolymph-Associated Biomineralization Ex-Vivo |
title | 3D Chitin Scaffolds of Marine Demosponge Origin for Biomimetic Mollusk Hemolymph-Associated Biomineralization Ex-Vivo |
title_full | 3D Chitin Scaffolds of Marine Demosponge Origin for Biomimetic Mollusk Hemolymph-Associated Biomineralization Ex-Vivo |
title_fullStr | 3D Chitin Scaffolds of Marine Demosponge Origin for Biomimetic Mollusk Hemolymph-Associated Biomineralization Ex-Vivo |
title_full_unstemmed | 3D Chitin Scaffolds of Marine Demosponge Origin for Biomimetic Mollusk Hemolymph-Associated Biomineralization Ex-Vivo |
title_short | 3D Chitin Scaffolds of Marine Demosponge Origin for Biomimetic Mollusk Hemolymph-Associated Biomineralization Ex-Vivo |
title_sort | 3d chitin scaffolds of marine demosponge origin for biomimetic mollusk hemolymph-associated biomineralization ex-vivo |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7074400/ https://www.ncbi.nlm.nih.gov/pubmed/32092907 http://dx.doi.org/10.3390/md18020123 |
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