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Molecular-Assisted Distinctness and Uniformity Testing Using SLAF-Sequencing Approach in Soybean

Distinctness, uniformity and stability (DUS) testing of cultivars through morphological descriptors is an important and compulsory part of soybean breeding. Molecular markers are usually more effective and accurate in describing the genetic features for the identification and purity assessment of cu...

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Autores principales: Zhang, Shengrui, Li, Bin, Chen, Ying, Shaibu, Abdulwahab S., Zheng, Hongkun, Sun, Junming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7074437/
https://www.ncbi.nlm.nih.gov/pubmed/32041312
http://dx.doi.org/10.3390/genes11020175
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author Zhang, Shengrui
Li, Bin
Chen, Ying
Shaibu, Abdulwahab S.
Zheng, Hongkun
Sun, Junming
author_facet Zhang, Shengrui
Li, Bin
Chen, Ying
Shaibu, Abdulwahab S.
Zheng, Hongkun
Sun, Junming
author_sort Zhang, Shengrui
collection PubMed
description Distinctness, uniformity and stability (DUS) testing of cultivars through morphological descriptors is an important and compulsory part of soybean breeding. Molecular markers are usually more effective and accurate in describing the genetic features for the identification and purity assessment of cultivars. In the present study, we assessed the distinctness and uniformity of five soybean cultivars using both single nucleotide polymorphism (SNP) markers developed by specific-locus amplified fragment sequencing (SLAF-seq) technology, and simple sequence repeat (SSR) markers. The phylogenetic tree and principal component analysis (PCA) from both the SLAF-seq and SSR methods showed a clear distinction among cultivars Zhonghuang 18, Zhonghuang 68 and Zhonghuang 35, while no clear distinction was observed between cultivars Zhonghuang 13 and Hedou 13. Using the SLAF-seq method, we determined the proportion of homozygous loci for the five soybean cultivars. The heterozygosity of each individual plant was estimated for the assessment of cultivar purity and the purity levels of the five soybean cultivars ranged from 91.89% to 93.96%. To further validate the applicability of the SLAF-seq approach for distinctness testing, we used the SNP information of 150 soybean cultivars with different origins. The cultivars were also distinguished clearly. Taken together, SLAF-seq can be used as an accurate and reliable method in the assessment of the distinctness and uniformity of soybean cultivars.
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spelling pubmed-70744372020-03-20 Molecular-Assisted Distinctness and Uniformity Testing Using SLAF-Sequencing Approach in Soybean Zhang, Shengrui Li, Bin Chen, Ying Shaibu, Abdulwahab S. Zheng, Hongkun Sun, Junming Genes (Basel) Article Distinctness, uniformity and stability (DUS) testing of cultivars through morphological descriptors is an important and compulsory part of soybean breeding. Molecular markers are usually more effective and accurate in describing the genetic features for the identification and purity assessment of cultivars. In the present study, we assessed the distinctness and uniformity of five soybean cultivars using both single nucleotide polymorphism (SNP) markers developed by specific-locus amplified fragment sequencing (SLAF-seq) technology, and simple sequence repeat (SSR) markers. The phylogenetic tree and principal component analysis (PCA) from both the SLAF-seq and SSR methods showed a clear distinction among cultivars Zhonghuang 18, Zhonghuang 68 and Zhonghuang 35, while no clear distinction was observed between cultivars Zhonghuang 13 and Hedou 13. Using the SLAF-seq method, we determined the proportion of homozygous loci for the five soybean cultivars. The heterozygosity of each individual plant was estimated for the assessment of cultivar purity and the purity levels of the five soybean cultivars ranged from 91.89% to 93.96%. To further validate the applicability of the SLAF-seq approach for distinctness testing, we used the SNP information of 150 soybean cultivars with different origins. The cultivars were also distinguished clearly. Taken together, SLAF-seq can be used as an accurate and reliable method in the assessment of the distinctness and uniformity of soybean cultivars. MDPI 2020-02-06 /pmc/articles/PMC7074437/ /pubmed/32041312 http://dx.doi.org/10.3390/genes11020175 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhang, Shengrui
Li, Bin
Chen, Ying
Shaibu, Abdulwahab S.
Zheng, Hongkun
Sun, Junming
Molecular-Assisted Distinctness and Uniformity Testing Using SLAF-Sequencing Approach in Soybean
title Molecular-Assisted Distinctness and Uniformity Testing Using SLAF-Sequencing Approach in Soybean
title_full Molecular-Assisted Distinctness and Uniformity Testing Using SLAF-Sequencing Approach in Soybean
title_fullStr Molecular-Assisted Distinctness and Uniformity Testing Using SLAF-Sequencing Approach in Soybean
title_full_unstemmed Molecular-Assisted Distinctness and Uniformity Testing Using SLAF-Sequencing Approach in Soybean
title_short Molecular-Assisted Distinctness and Uniformity Testing Using SLAF-Sequencing Approach in Soybean
title_sort molecular-assisted distinctness and uniformity testing using slaf-sequencing approach in soybean
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7074437/
https://www.ncbi.nlm.nih.gov/pubmed/32041312
http://dx.doi.org/10.3390/genes11020175
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