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Nanosensors-Assisted Quantitative Analysis of Biochemical Processes in Droplets
Here, we present a miniaturized lab-on-a-chip detecting system for an all-electric and label-free analysis of the emulsion droplets incorporating the nanoscopic silicon nanowires-based field-effect transistors (FETs). We specifically focus on the analysis of β-galactosidase e.g., activity, which is...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7074628/ https://www.ncbi.nlm.nih.gov/pubmed/31991863 http://dx.doi.org/10.3390/mi11020138 |
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author | Belyaev, Dmitry Schütt, Julian Ibarlucea, Bergoi Rim, Taiuk Baraban, Larysa Cuniberti, Gianaurelio |
author_facet | Belyaev, Dmitry Schütt, Julian Ibarlucea, Bergoi Rim, Taiuk Baraban, Larysa Cuniberti, Gianaurelio |
author_sort | Belyaev, Dmitry |
collection | PubMed |
description | Here, we present a miniaturized lab-on-a-chip detecting system for an all-electric and label-free analysis of the emulsion droplets incorporating the nanoscopic silicon nanowires-based field-effect transistors (FETs). We specifically focus on the analysis of β-galactosidase e.g., activity, which is an important enzyme of the glycolysis metabolic pathway. Furthermore, the efficiency of the synthesis and action of β-galactosidase can be one of the markers for several diseases, e.g., cancer, hyper/hypoglycemia, cell senescence, or other disruptions in cell functioning. We measure the reaction and reaction kinetics-associated shift of the source-to-drain current I(sd) in the system, which is caused by the change of the ionic strength of the microenvironment. With these results, we demonstrate that the ion-sensitive FETs are able to sense the interior of the aqueous reactors; thus, the conjunction of miniature nanosensors and droplet-based microfluidic systems conceptually opens a new route toward a sensitive, optics-less analysis of biochemical processes. |
format | Online Article Text |
id | pubmed-7074628 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-70746282020-03-20 Nanosensors-Assisted Quantitative Analysis of Biochemical Processes in Droplets Belyaev, Dmitry Schütt, Julian Ibarlucea, Bergoi Rim, Taiuk Baraban, Larysa Cuniberti, Gianaurelio Micromachines (Basel) Article Here, we present a miniaturized lab-on-a-chip detecting system for an all-electric and label-free analysis of the emulsion droplets incorporating the nanoscopic silicon nanowires-based field-effect transistors (FETs). We specifically focus on the analysis of β-galactosidase e.g., activity, which is an important enzyme of the glycolysis metabolic pathway. Furthermore, the efficiency of the synthesis and action of β-galactosidase can be one of the markers for several diseases, e.g., cancer, hyper/hypoglycemia, cell senescence, or other disruptions in cell functioning. We measure the reaction and reaction kinetics-associated shift of the source-to-drain current I(sd) in the system, which is caused by the change of the ionic strength of the microenvironment. With these results, we demonstrate that the ion-sensitive FETs are able to sense the interior of the aqueous reactors; thus, the conjunction of miniature nanosensors and droplet-based microfluidic systems conceptually opens a new route toward a sensitive, optics-less analysis of biochemical processes. MDPI 2020-01-26 /pmc/articles/PMC7074628/ /pubmed/31991863 http://dx.doi.org/10.3390/mi11020138 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Belyaev, Dmitry Schütt, Julian Ibarlucea, Bergoi Rim, Taiuk Baraban, Larysa Cuniberti, Gianaurelio Nanosensors-Assisted Quantitative Analysis of Biochemical Processes in Droplets |
title | Nanosensors-Assisted Quantitative Analysis of Biochemical Processes in Droplets |
title_full | Nanosensors-Assisted Quantitative Analysis of Biochemical Processes in Droplets |
title_fullStr | Nanosensors-Assisted Quantitative Analysis of Biochemical Processes in Droplets |
title_full_unstemmed | Nanosensors-Assisted Quantitative Analysis of Biochemical Processes in Droplets |
title_short | Nanosensors-Assisted Quantitative Analysis of Biochemical Processes in Droplets |
title_sort | nanosensors-assisted quantitative analysis of biochemical processes in droplets |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7074628/ https://www.ncbi.nlm.nih.gov/pubmed/31991863 http://dx.doi.org/10.3390/mi11020138 |
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