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A Miniature Fibre-Optic Raman Probe Fabricated by Ultrafast Laser-Assisted Etching

Optical biopsy describes a range of medical procedures in which light is used to investigate disease in the body, often in hard-to-reach regions via optical fibres. Optical biopsies can reveal a multitude of diagnostic information to aid therapeutic diagnosis and treatment with higher specificity an...

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Autores principales: Ross, Calum A., MacLachlan, David G., Smith, Brian J. E., Beck, Rainer J., Shephard, Jonathan D., Weston, Nick, Thomson, Robert R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7074630/
https://www.ncbi.nlm.nih.gov/pubmed/32053957
http://dx.doi.org/10.3390/mi11020185
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author Ross, Calum A.
MacLachlan, David G.
Smith, Brian J. E.
Beck, Rainer J.
Shephard, Jonathan D.
Weston, Nick
Thomson, Robert R.
author_facet Ross, Calum A.
MacLachlan, David G.
Smith, Brian J. E.
Beck, Rainer J.
Shephard, Jonathan D.
Weston, Nick
Thomson, Robert R.
author_sort Ross, Calum A.
collection PubMed
description Optical biopsy describes a range of medical procedures in which light is used to investigate disease in the body, often in hard-to-reach regions via optical fibres. Optical biopsies can reveal a multitude of diagnostic information to aid therapeutic diagnosis and treatment with higher specificity and shorter delay than traditional surgical techniques. One specific type of optical biopsy relies on Raman spectroscopy to differentiate tissue types at the molecular level and has been used successfully to stage cancer. However, complex micro-optical systems are usually needed at the distal end to optimise the signal-to-noise properties of the Raman signal collected. Manufacturing these devices, particularly in a way suitable for large scale adoption, remains a critical challenge. In this paper, we describe a novel fibre-fed micro-optic system designed for efficient signal delivery and collection during a Raman spectroscopy-based optical biopsy. Crucially, we fabricate the device using a direct-laser-writing technique known as ultrafast laser-assisted etching which is scalable and allows components to be aligned passively. The Raman probe has a sub-millimetre diameter and offers confocal signal collection with 71.3% ± 1.5% collection efficiency over a 0.8 numerical aperture. Proof of concept spectral measurements were performed on mouse intestinal tissue and compared with results obtained using a commercial Raman microscope.
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spelling pubmed-70746302020-03-20 A Miniature Fibre-Optic Raman Probe Fabricated by Ultrafast Laser-Assisted Etching Ross, Calum A. MacLachlan, David G. Smith, Brian J. E. Beck, Rainer J. Shephard, Jonathan D. Weston, Nick Thomson, Robert R. Micromachines (Basel) Article Optical biopsy describes a range of medical procedures in which light is used to investigate disease in the body, often in hard-to-reach regions via optical fibres. Optical biopsies can reveal a multitude of diagnostic information to aid therapeutic diagnosis and treatment with higher specificity and shorter delay than traditional surgical techniques. One specific type of optical biopsy relies on Raman spectroscopy to differentiate tissue types at the molecular level and has been used successfully to stage cancer. However, complex micro-optical systems are usually needed at the distal end to optimise the signal-to-noise properties of the Raman signal collected. Manufacturing these devices, particularly in a way suitable for large scale adoption, remains a critical challenge. In this paper, we describe a novel fibre-fed micro-optic system designed for efficient signal delivery and collection during a Raman spectroscopy-based optical biopsy. Crucially, we fabricate the device using a direct-laser-writing technique known as ultrafast laser-assisted etching which is scalable and allows components to be aligned passively. The Raman probe has a sub-millimetre diameter and offers confocal signal collection with 71.3% ± 1.5% collection efficiency over a 0.8 numerical aperture. Proof of concept spectral measurements were performed on mouse intestinal tissue and compared with results obtained using a commercial Raman microscope. MDPI 2020-02-11 /pmc/articles/PMC7074630/ /pubmed/32053957 http://dx.doi.org/10.3390/mi11020185 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ross, Calum A.
MacLachlan, David G.
Smith, Brian J. E.
Beck, Rainer J.
Shephard, Jonathan D.
Weston, Nick
Thomson, Robert R.
A Miniature Fibre-Optic Raman Probe Fabricated by Ultrafast Laser-Assisted Etching
title A Miniature Fibre-Optic Raman Probe Fabricated by Ultrafast Laser-Assisted Etching
title_full A Miniature Fibre-Optic Raman Probe Fabricated by Ultrafast Laser-Assisted Etching
title_fullStr A Miniature Fibre-Optic Raman Probe Fabricated by Ultrafast Laser-Assisted Etching
title_full_unstemmed A Miniature Fibre-Optic Raman Probe Fabricated by Ultrafast Laser-Assisted Etching
title_short A Miniature Fibre-Optic Raman Probe Fabricated by Ultrafast Laser-Assisted Etching
title_sort miniature fibre-optic raman probe fabricated by ultrafast laser-assisted etching
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7074630/
https://www.ncbi.nlm.nih.gov/pubmed/32053957
http://dx.doi.org/10.3390/mi11020185
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