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A Quantitative Study of the Secondary Acoustic Radiation Force on Biological Cells during Acoustophoresis

We investigate cell-particle secondary acoustic radiation forces in a plain ultrasonic standing wave field inside a microfluidic channel. The effect of secondary acoustic radiation forces on biological cells is measured in a location between a pressure node and a pressure anti-node and the result is...

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Autores principales: Saeidi, Davood, Saghafian, Mohsen, Haghjooy Javanmard, Shaghayegh, Wiklund, Martin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7074662/
https://www.ncbi.nlm.nih.gov/pubmed/32019234
http://dx.doi.org/10.3390/mi11020152
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author Saeidi, Davood
Saghafian, Mohsen
Haghjooy Javanmard, Shaghayegh
Wiklund, Martin
author_facet Saeidi, Davood
Saghafian, Mohsen
Haghjooy Javanmard, Shaghayegh
Wiklund, Martin
author_sort Saeidi, Davood
collection PubMed
description We investigate cell-particle secondary acoustic radiation forces in a plain ultrasonic standing wave field inside a microfluidic channel. The effect of secondary acoustic radiation forces on biological cells is measured in a location between a pressure node and a pressure anti-node and the result is compared with theory by considering both compressibility and density dependent effects. The secondary acoustic force between motile red blood cells (RBCs) and MCF-7 cells and fixed 20 µm silica beads is investigated in a half-wavelength wide microchannel actuated at 2 MHz ultrasonic frequency. Our study shows that the secondary acoustic force between cells in acoustofluidic devices could play an important role for cell separation, sorting, and trapping purposes. Our results also demonstrate the possibility to isolate individual cells at trapping positions provided by silica beads immobilized and adhered to the microchannel bottom. We conclude that during certain experimental conditions, the secondary acoustic force acting on biological cells can dominate over the primary acoustic radiation force, which could open up for new microscale acoustofluidic methods.
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spelling pubmed-70746622020-03-20 A Quantitative Study of the Secondary Acoustic Radiation Force on Biological Cells during Acoustophoresis Saeidi, Davood Saghafian, Mohsen Haghjooy Javanmard, Shaghayegh Wiklund, Martin Micromachines (Basel) Article We investigate cell-particle secondary acoustic radiation forces in a plain ultrasonic standing wave field inside a microfluidic channel. The effect of secondary acoustic radiation forces on biological cells is measured in a location between a pressure node and a pressure anti-node and the result is compared with theory by considering both compressibility and density dependent effects. The secondary acoustic force between motile red blood cells (RBCs) and MCF-7 cells and fixed 20 µm silica beads is investigated in a half-wavelength wide microchannel actuated at 2 MHz ultrasonic frequency. Our study shows that the secondary acoustic force between cells in acoustofluidic devices could play an important role for cell separation, sorting, and trapping purposes. Our results also demonstrate the possibility to isolate individual cells at trapping positions provided by silica beads immobilized and adhered to the microchannel bottom. We conclude that during certain experimental conditions, the secondary acoustic force acting on biological cells can dominate over the primary acoustic radiation force, which could open up for new microscale acoustofluidic methods. MDPI 2020-01-30 /pmc/articles/PMC7074662/ /pubmed/32019234 http://dx.doi.org/10.3390/mi11020152 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Saeidi, Davood
Saghafian, Mohsen
Haghjooy Javanmard, Shaghayegh
Wiklund, Martin
A Quantitative Study of the Secondary Acoustic Radiation Force on Biological Cells during Acoustophoresis
title A Quantitative Study of the Secondary Acoustic Radiation Force on Biological Cells during Acoustophoresis
title_full A Quantitative Study of the Secondary Acoustic Radiation Force on Biological Cells during Acoustophoresis
title_fullStr A Quantitative Study of the Secondary Acoustic Radiation Force on Biological Cells during Acoustophoresis
title_full_unstemmed A Quantitative Study of the Secondary Acoustic Radiation Force on Biological Cells during Acoustophoresis
title_short A Quantitative Study of the Secondary Acoustic Radiation Force on Biological Cells during Acoustophoresis
title_sort quantitative study of the secondary acoustic radiation force on biological cells during acoustophoresis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7074662/
https://www.ncbi.nlm.nih.gov/pubmed/32019234
http://dx.doi.org/10.3390/mi11020152
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