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Continuous Microfluidic Purification of DNA Using Magnetophoresis
Automatic microfluidic purification of nucleic acid is predictable to reduce the input of original samples and improve the throughput of library preparation for sequencing. Here, we propose a novel microfluidic system using an external NdFeB magnet to isolate DNA from the polymerase chain reaction (...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7074667/ https://www.ncbi.nlm.nih.gov/pubmed/32054004 http://dx.doi.org/10.3390/mi11020187 |
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author | Xu, Ying Zhang, Zhen Su, Zhen Zhou, Xiaoxiang Han, Xiaoming Liu, Quanjun |
author_facet | Xu, Ying Zhang, Zhen Su, Zhen Zhou, Xiaoxiang Han, Xiaoming Liu, Quanjun |
author_sort | Xu, Ying |
collection | PubMed |
description | Automatic microfluidic purification of nucleic acid is predictable to reduce the input of original samples and improve the throughput of library preparation for sequencing. Here, we propose a novel microfluidic system using an external NdFeB magnet to isolate DNA from the polymerase chain reaction (PCR) mixture. The DNA was purified and isolated when the DNA-carrying beads transported to the interface of multi-laminar flow under the influence of magnetic field. Prior to the DNA recovery experiments, COMSOL simulations were carried out to study the relationship between trajectory of beads and magnet positions as well as fluid velocities. Afterwards, the experiments to study the influence of varying velocities and input of samples on the DNA recovery were conducted. Compared to experimental results, the relative error of the final position of beads is less than 10%. The recovery efficiency decreases with increase of input or fluid velocity, and the maximum DNA recovery efficiency is 98.4% with input of l00 ng DNA at fluid velocity of 1.373 mm/s. The results show that simulations significantly reduce the time for parameter adjustment in experiments. In addition, this platform uses a basic two-layer chip to realize automatic DNA isolation without any other liquid switch value or magnet controller. |
format | Online Article Text |
id | pubmed-7074667 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-70746672020-03-20 Continuous Microfluidic Purification of DNA Using Magnetophoresis Xu, Ying Zhang, Zhen Su, Zhen Zhou, Xiaoxiang Han, Xiaoming Liu, Quanjun Micromachines (Basel) Article Automatic microfluidic purification of nucleic acid is predictable to reduce the input of original samples and improve the throughput of library preparation for sequencing. Here, we propose a novel microfluidic system using an external NdFeB magnet to isolate DNA from the polymerase chain reaction (PCR) mixture. The DNA was purified and isolated when the DNA-carrying beads transported to the interface of multi-laminar flow under the influence of magnetic field. Prior to the DNA recovery experiments, COMSOL simulations were carried out to study the relationship between trajectory of beads and magnet positions as well as fluid velocities. Afterwards, the experiments to study the influence of varying velocities and input of samples on the DNA recovery were conducted. Compared to experimental results, the relative error of the final position of beads is less than 10%. The recovery efficiency decreases with increase of input or fluid velocity, and the maximum DNA recovery efficiency is 98.4% with input of l00 ng DNA at fluid velocity of 1.373 mm/s. The results show that simulations significantly reduce the time for parameter adjustment in experiments. In addition, this platform uses a basic two-layer chip to realize automatic DNA isolation without any other liquid switch value or magnet controller. MDPI 2020-02-11 /pmc/articles/PMC7074667/ /pubmed/32054004 http://dx.doi.org/10.3390/mi11020187 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Xu, Ying Zhang, Zhen Su, Zhen Zhou, Xiaoxiang Han, Xiaoming Liu, Quanjun Continuous Microfluidic Purification of DNA Using Magnetophoresis |
title | Continuous Microfluidic Purification of DNA Using Magnetophoresis |
title_full | Continuous Microfluidic Purification of DNA Using Magnetophoresis |
title_fullStr | Continuous Microfluidic Purification of DNA Using Magnetophoresis |
title_full_unstemmed | Continuous Microfluidic Purification of DNA Using Magnetophoresis |
title_short | Continuous Microfluidic Purification of DNA Using Magnetophoresis |
title_sort | continuous microfluidic purification of dna using magnetophoresis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7074667/ https://www.ncbi.nlm.nih.gov/pubmed/32054004 http://dx.doi.org/10.3390/mi11020187 |
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