Cargando…

Continuous Microfluidic Purification of DNA Using Magnetophoresis

Automatic microfluidic purification of nucleic acid is predictable to reduce the input of original samples and improve the throughput of library preparation for sequencing. Here, we propose a novel microfluidic system using an external NdFeB magnet to isolate DNA from the polymerase chain reaction (...

Descripción completa

Detalles Bibliográficos
Autores principales:	Xu, Ying, Zhang, Zhen, Su, Zhen, Zhou, Xiaoxiang, Han, Xiaoming, Liu, Quanjun
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2020
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7074667/ https://www.ncbi.nlm.nih.gov/pubmed/32054004 http://dx.doi.org/10.3390/mi11020187

Ejemplares similares

Magnetophoresis in Centrifugal Microfluidics at Continuous Rotation for Nucleic Acid Extraction
por: Hin, Sebastian, et al.
Publicado: (2022)

Multigene model for predicting metastatic prostate cancer using circulating tumor cells by microfluidic magnetophoresis
por: Cho, Hyungseok, et al.
Publicado: (2020)

Comparison of Multiple Displacement Amplification (MDA) and Multiple Annealing and Looping-Based Amplification Cycles (MALBAC) in Limited DNA Sequencing Based on Tube and Droplet
por: Zhou, Xiaoxiang, et al.
Publicado: (2020)

Evaluation of Positive and Negative Methods for Isolation of Circulating Tumor Cells by Lateral Magnetophoresis
por: Kang, Haeli, et al.
Publicado: (2019)

Numerical Analysis of Bead Magnetophoresis from Flowing Blood in a Continuous-Flow Microchannel: Implications to the Bead-Fluid Interactions
por: Gómez-Pastora, Jenifer, et al.
Publicado: (2019)

Improved Anisotropic Thermoelectric Behavior of Poly(3,4-ethylenedioxythiophene):Poly(styrenesulfonate) via Magnetophoresis
por: Zarubin, Vera A., et al.
Publicado: (2018)

Turning Diamagnetic Microbes into Multinary Micro-Magnets: Magnetophoresis and Spatio-Temporal Manipulation of Individual Living Cells
por: Lee, Hojae, et al.
Publicado: (2016)

Investigation of a two‐step device implementing magnetophoresis and dielectrophoresis for separation of circulating tumor cells from blood cells
por: Shamloo, Amir, et al.
Publicado: (2020)

Continuous On-Chip Cell Washing Using Viscoelastic Microfluidics
por: Lim, Hyunjung, et al.
Publicado: (2023)

Deterministic Lateral Displacement Microfluidic Chip for Minicell Purification
por: Sherbaz, Ahmad, et al.
Publicado: (2022)

Purification and preconcentration of genomic DNA from whole cell lysates using photoactivated polycarbonate (PPC) microfluidic chips
por: Witek, Małgorzata A., et al.
Publicado: (2006)

Rapid and label-free microfluidic neutrophil purification and phenotyping in diabetes mellitus
por: Hou, Han Wei, et al.
Publicado: (2016)

Microfluidic Purification and Concentration of Malignant Pleural Effusions for Improved Molecular and Cytomorphological Diagnostics
por: Che, James, et al.
Publicado: (2013)

One-Step Generation and Purification of Cell-Encapsulated Hydrogel Microsphere With an Easily Assembled Microfluidic Device
por: Zhang, Tao, et al.
Publicado: (2022)

Amalgamation of diverse hydrodynamic effects with novel triple-sided membrane valves for developing a microfluidic device for filterless and continuous water purification
por: Prabhakar, Amit, et al.
Publicado: (2021)

Continuous Flow Microfluidic Bioparticle Concentrator
por: Martel, Joseph M., et al.
Publicado: (2015)

Magnetic Bead Chain-Based Continuous-Flow DNA Extraction for Microfluidic PCR Detection of Salmonella
por: Wang, Yuhe, et al.
Publicado: (2021)

Different Strategies for the Microfluidic Purification of Antibiotics from Food: A Comparative Study
por: Lunelli, Lorenzo, et al.
Publicado: (2023)

Continuous microfluidic fabrication of polypyrrole nanoparticles
por: Effati, Elham, et al.
Publicado: (2019)

Purification and Characterization of Resistant Dextrin
por: Zhen, Yuanhang, et al.
Publicado: (2021)

Clogging-free microfluidics for continuous size-based separation of microparticles
por: Yoon, Yousang, et al.
Publicado: (2016)

Continuous microfluidic assortment of interactive ligands (CMAIL)
por: Hsiao, Yi-Hsing, et al.
Publicado: (2016)

Stimuli-Responsive Reagent System for Enabling Microfluidic Immunoassays with Biomarker Purification and Enrichment
por: Hoffman, John M., et al.
Publicado: (2014)

Exploiting biased reptation for continuous flow preparative DNA fractionation in a versatile microfluidic platform
por: Gumuscu, Burcu, et al.
Publicado: (2017)

A Continuous Flow-through Microfluidic Device for Electrical Lysis of Cells
por: Lo, Ying-Jie, et al.
Publicado: (2019)

Microfluidic Synthesis and Purification of Magnetoliposomes for Potential Applications in the Gastrointestinal Delivery of Difficult-to-Transport Drugs
por: Torres, Carlos E., et al.
Publicado: (2022)

Continuous artificial synthesis of glucose precursor using enzyme-immobilized microfluidic reactors
por: Zhu, Yujiao, et al.
Publicado: (2019)

Development of a Microfluidic Platform for R-Phycoerythrin Purification Using an Aqueous Micellar Two-Phase System
por: Seručnik, Mojca, et al.
Publicado: (2020)

Sequence-selective purification of biological RNAs using DNA nanoswitches
por: Zhou, Lifeng, et al.
Publicado: (2021)

A Dual-Channel Microfluidic Chip for Single Tobacco Protoplast Isolation and Dynamic Capture
por: Zhang, Huali, et al.
Publicado: (2022)

Acousto-microfluidics for screening of ssDNA aptamer
por: Park, Jee-Woong, et al.
Publicado: (2016)

Development of an Optically Induced Dielectrophoresis (ODEP) Microfluidic System for High-Performance Isolation and Purification of Bacteria
por: Chu, Po-Yu, et al.
Publicado: (2023)

Fabrication of Microfluidic Devices for Continuously Monitoring Yeast Aging
por: O’Laughlin, Richard, et al.
Publicado: (2023)

Purification and Characterization of Recombinant Human Lysozyme from Eggs of Transgenic Chickens
por: Wu, Hanyu, et al.
Publicado: (2015)

Continuous Determination of Glucose Using a Membraneless, Microfluidic Enzymatic Biofuel Cell
por: Khan, Haroon, et al.
Publicado: (2020)

Purification of Sesquiterpenes from Saussurea Lappa Roots by High Speed Counter Current Chromatography
por: Cao, Kun, et al.
Publicado: (2019)

Purification of Ustiloxins A and B from Rice False Smut Balls by Macroporous Resins
por: Shan, Tijiang, et al.
Publicado: (2013)

Continuous Affinity Purification of Adeno-Associated Virus Using Periodic Counter-Current Chromatography
por: Mendes, João P., et al.
Publicado: (2022)

Purification of specific DNA species using the CRISPR system
por: Fujita, Toshitsugu, et al.
Publicado: (2019)

Neural network execution using nicked DNA and microfluidics
por: Solanki, Arnav, et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_adfk2ik3g7i8lso2t9gbu35t4r