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Lab-on-Chip Platform for Culturing and Dynamic Evaluation of Cells Development
This paper presents a full-featured microfluidic platform ensuring long-term culturing and behavioral analysis of the radically different biological micro-objects. The platform uses all-glass lab-chips and MEMS-based components providing dedicated micro-aquatic habitats for the cells, as well as the...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7074672/ https://www.ncbi.nlm.nih.gov/pubmed/32074950 http://dx.doi.org/10.3390/mi11020196 |
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author | Podwin, Agnieszka Lizanets, Danylo Przystupski, Dawid Kubicki, Wojciech Śniadek, Patrycja Kulbacka, Julita Wymysłowski, Artur Walczak, Rafał Dziuban, Jan A. |
author_facet | Podwin, Agnieszka Lizanets, Danylo Przystupski, Dawid Kubicki, Wojciech Śniadek, Patrycja Kulbacka, Julita Wymysłowski, Artur Walczak, Rafał Dziuban, Jan A. |
author_sort | Podwin, Agnieszka |
collection | PubMed |
description | This paper presents a full-featured microfluidic platform ensuring long-term culturing and behavioral analysis of the radically different biological micro-objects. The platform uses all-glass lab-chips and MEMS-based components providing dedicated micro-aquatic habitats for the cells, as well as their intentional disturbances on-chip. Specially developed software was implemented to characterize the micro-objects metrologically in terms of population growth and cells’ size, shape, or migration activity. To date, the platform has been successfully applied for the culturing of freshwater microorganisms, fungi, cancer cells, and animal oocytes, showing their notable population growth, high mobility, and taxis mechanisms. For instance, circa 100% expansion of porcine oocytes cells, as well as nearly five-fold increase in E. gracilis population, has been achieved. These results are a good base to conduct further research on the platform versatile applications. |
format | Online Article Text |
id | pubmed-7074672 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-70746722020-03-20 Lab-on-Chip Platform for Culturing and Dynamic Evaluation of Cells Development Podwin, Agnieszka Lizanets, Danylo Przystupski, Dawid Kubicki, Wojciech Śniadek, Patrycja Kulbacka, Julita Wymysłowski, Artur Walczak, Rafał Dziuban, Jan A. Micromachines (Basel) Article This paper presents a full-featured microfluidic platform ensuring long-term culturing and behavioral analysis of the radically different biological micro-objects. The platform uses all-glass lab-chips and MEMS-based components providing dedicated micro-aquatic habitats for the cells, as well as their intentional disturbances on-chip. Specially developed software was implemented to characterize the micro-objects metrologically in terms of population growth and cells’ size, shape, or migration activity. To date, the platform has been successfully applied for the culturing of freshwater microorganisms, fungi, cancer cells, and animal oocytes, showing their notable population growth, high mobility, and taxis mechanisms. For instance, circa 100% expansion of porcine oocytes cells, as well as nearly five-fold increase in E. gracilis population, has been achieved. These results are a good base to conduct further research on the platform versatile applications. MDPI 2020-02-14 /pmc/articles/PMC7074672/ /pubmed/32074950 http://dx.doi.org/10.3390/mi11020196 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Podwin, Agnieszka Lizanets, Danylo Przystupski, Dawid Kubicki, Wojciech Śniadek, Patrycja Kulbacka, Julita Wymysłowski, Artur Walczak, Rafał Dziuban, Jan A. Lab-on-Chip Platform for Culturing and Dynamic Evaluation of Cells Development |
title | Lab-on-Chip Platform for Culturing and Dynamic Evaluation of Cells Development |
title_full | Lab-on-Chip Platform for Culturing and Dynamic Evaluation of Cells Development |
title_fullStr | Lab-on-Chip Platform for Culturing and Dynamic Evaluation of Cells Development |
title_full_unstemmed | Lab-on-Chip Platform for Culturing and Dynamic Evaluation of Cells Development |
title_short | Lab-on-Chip Platform for Culturing and Dynamic Evaluation of Cells Development |
title_sort | lab-on-chip platform for culturing and dynamic evaluation of cells development |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7074672/ https://www.ncbi.nlm.nih.gov/pubmed/32074950 http://dx.doi.org/10.3390/mi11020196 |
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