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Methylcellulose Hydrogel with Melissa officinalis Essential Oil as a Potential Treatment for Oral Candidiasis

Candida spp. are the most prevalent fungi of the human microbiota and are opportunistic pathogens that can cause oral candidiasis. Management of such infections is limited due to the low number of antifungal drugs available, their relatively high toxicity and the emergence of antifungal resistance....

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Autores principales: Serra, Elisa, Saubade, Fabien, Ligorio, Cosimo, Whitehead, Kathryn, Sloan, Alastair, Williams, David W., Hidalgo-Bastida, Araida, Verran, Joanna, Malic, Sladjana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7074814/
https://www.ncbi.nlm.nih.gov/pubmed/32041100
http://dx.doi.org/10.3390/microorganisms8020215
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author Serra, Elisa
Saubade, Fabien
Ligorio, Cosimo
Whitehead, Kathryn
Sloan, Alastair
Williams, David W.
Hidalgo-Bastida, Araida
Verran, Joanna
Malic, Sladjana
author_facet Serra, Elisa
Saubade, Fabien
Ligorio, Cosimo
Whitehead, Kathryn
Sloan, Alastair
Williams, David W.
Hidalgo-Bastida, Araida
Verran, Joanna
Malic, Sladjana
author_sort Serra, Elisa
collection PubMed
description Candida spp. are the most prevalent fungi of the human microbiota and are opportunistic pathogens that can cause oral candidiasis. Management of such infections is limited due to the low number of antifungal drugs available, their relatively high toxicity and the emergence of antifungal resistance. Therefore, much interest in the antimicrobial potential of natural compounds has recently been evident. The use of hydrogels in the delivery of biocides has been explored due to their biocompatibility, ease with drug encapsulation, and due to their potential to confer mechanical and structural properties similar to biological tissue. Methylcellulose hydrogels (10% (w/v)) with 1% (v/v) and 2% (v/v) Melissa officinalis oil were synthesised. The rheological properties and gelation time of the hydrogels were evaluated. Antimicrobial action, the antifungal potential and ability to displace Candida were determined. Rheological tests revealed that the hydrogel jellified in three minutes at 37 °C. Loaded hydrogels successfully inhibited Candida albicans growth as evident by zone of inhibition and time-kill assays. A significant reduction in retained C. albicans was demonstrated with the hydrogel at 2% Melissa officinalis concentration. This work demonstrated that an essential oil-loaded hydrogel had the potential to provide a novel antimicrobial therapy for the treatment of oral candidiasis.
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spelling pubmed-70748142020-03-20 Methylcellulose Hydrogel with Melissa officinalis Essential Oil as a Potential Treatment for Oral Candidiasis Serra, Elisa Saubade, Fabien Ligorio, Cosimo Whitehead, Kathryn Sloan, Alastair Williams, David W. Hidalgo-Bastida, Araida Verran, Joanna Malic, Sladjana Microorganisms Article Candida spp. are the most prevalent fungi of the human microbiota and are opportunistic pathogens that can cause oral candidiasis. Management of such infections is limited due to the low number of antifungal drugs available, their relatively high toxicity and the emergence of antifungal resistance. Therefore, much interest in the antimicrobial potential of natural compounds has recently been evident. The use of hydrogels in the delivery of biocides has been explored due to their biocompatibility, ease with drug encapsulation, and due to their potential to confer mechanical and structural properties similar to biological tissue. Methylcellulose hydrogels (10% (w/v)) with 1% (v/v) and 2% (v/v) Melissa officinalis oil were synthesised. The rheological properties and gelation time of the hydrogels were evaluated. Antimicrobial action, the antifungal potential and ability to displace Candida were determined. Rheological tests revealed that the hydrogel jellified in three minutes at 37 °C. Loaded hydrogels successfully inhibited Candida albicans growth as evident by zone of inhibition and time-kill assays. A significant reduction in retained C. albicans was demonstrated with the hydrogel at 2% Melissa officinalis concentration. This work demonstrated that an essential oil-loaded hydrogel had the potential to provide a novel antimicrobial therapy for the treatment of oral candidiasis. MDPI 2020-02-06 /pmc/articles/PMC7074814/ /pubmed/32041100 http://dx.doi.org/10.3390/microorganisms8020215 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Serra, Elisa
Saubade, Fabien
Ligorio, Cosimo
Whitehead, Kathryn
Sloan, Alastair
Williams, David W.
Hidalgo-Bastida, Araida
Verran, Joanna
Malic, Sladjana
Methylcellulose Hydrogel with Melissa officinalis Essential Oil as a Potential Treatment for Oral Candidiasis
title Methylcellulose Hydrogel with Melissa officinalis Essential Oil as a Potential Treatment for Oral Candidiasis
title_full Methylcellulose Hydrogel with Melissa officinalis Essential Oil as a Potential Treatment for Oral Candidiasis
title_fullStr Methylcellulose Hydrogel with Melissa officinalis Essential Oil as a Potential Treatment for Oral Candidiasis
title_full_unstemmed Methylcellulose Hydrogel with Melissa officinalis Essential Oil as a Potential Treatment for Oral Candidiasis
title_short Methylcellulose Hydrogel with Melissa officinalis Essential Oil as a Potential Treatment for Oral Candidiasis
title_sort methylcellulose hydrogel with melissa officinalis essential oil as a potential treatment for oral candidiasis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7074814/
https://www.ncbi.nlm.nih.gov/pubmed/32041100
http://dx.doi.org/10.3390/microorganisms8020215
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