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Preliminary Survey of Alternaria Toxins Reduction during Fermentation of Whole Wheat Dough

The aim of this study was to investigate the fate of the most common Alternaria toxins found in wheat—tenuazonic acid (TeA), alternariol (AOH), and alternariol monomethyl ether (AME) during sourdough processing. For this purpose, spiked whole wheat flour, 3% sourdough starter, 0.5% of baker’s yeast,...

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Autores principales: Janić Hajnal, Elizabet, Pezo, Lato, Orčić, Dejan, Šarić, Ljubiša, Plavšić, Dragana, Kos, Jovana, Mastilović, Jasna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7074859/
https://www.ncbi.nlm.nih.gov/pubmed/32098250
http://dx.doi.org/10.3390/microorganisms8020303
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author Janić Hajnal, Elizabet
Pezo, Lato
Orčić, Dejan
Šarić, Ljubiša
Plavšić, Dragana
Kos, Jovana
Mastilović, Jasna
author_facet Janić Hajnal, Elizabet
Pezo, Lato
Orčić, Dejan
Šarić, Ljubiša
Plavšić, Dragana
Kos, Jovana
Mastilović, Jasna
author_sort Janić Hajnal, Elizabet
collection PubMed
description The aim of this study was to investigate the fate of the most common Alternaria toxins found in wheat—tenuazonic acid (TeA), alternariol (AOH), and alternariol monomethyl ether (AME) during sourdough processing. For this purpose, spiked whole wheat flour, 3% sourdough starter, 0.5% of baker’s yeast, and 105% of water calculated on flour weight as a base were used as raw materials. Spiked whole wheat dough was fermented for 4 h, 8 h, 12 h, 24 h, and 48 h at 25 °C, and at each point the fermented dough samples were taken, frozen, lyophilized, grounded, and stored until further analysis. To study the effect of sourdough processing on TeA, AOH and AME content, the validated method of high-performance liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) for these mycotoxins was used. Mathematical models of Alternaria toxins reduction were developed in the form of Four Parameter Logistic Regression function. The maximum reduction of TeA, AOH, and AME levels was archived at 48 h of dough fermentation at 25 °C compared with dough after kneading (0 h). Under these conditions, a reduction of the toxin levels of 60.3 %, 41.5%, and 24.1% was observed for TeA, AOH, and AME, respectively.
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spelling pubmed-70748592020-03-20 Preliminary Survey of Alternaria Toxins Reduction during Fermentation of Whole Wheat Dough Janić Hajnal, Elizabet Pezo, Lato Orčić, Dejan Šarić, Ljubiša Plavšić, Dragana Kos, Jovana Mastilović, Jasna Microorganisms Communication The aim of this study was to investigate the fate of the most common Alternaria toxins found in wheat—tenuazonic acid (TeA), alternariol (AOH), and alternariol monomethyl ether (AME) during sourdough processing. For this purpose, spiked whole wheat flour, 3% sourdough starter, 0.5% of baker’s yeast, and 105% of water calculated on flour weight as a base were used as raw materials. Spiked whole wheat dough was fermented for 4 h, 8 h, 12 h, 24 h, and 48 h at 25 °C, and at each point the fermented dough samples were taken, frozen, lyophilized, grounded, and stored until further analysis. To study the effect of sourdough processing on TeA, AOH and AME content, the validated method of high-performance liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) for these mycotoxins was used. Mathematical models of Alternaria toxins reduction were developed in the form of Four Parameter Logistic Regression function. The maximum reduction of TeA, AOH, and AME levels was archived at 48 h of dough fermentation at 25 °C compared with dough after kneading (0 h). Under these conditions, a reduction of the toxin levels of 60.3 %, 41.5%, and 24.1% was observed for TeA, AOH, and AME, respectively. MDPI 2020-02-21 /pmc/articles/PMC7074859/ /pubmed/32098250 http://dx.doi.org/10.3390/microorganisms8020303 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Janić Hajnal, Elizabet
Pezo, Lato
Orčić, Dejan
Šarić, Ljubiša
Plavšić, Dragana
Kos, Jovana
Mastilović, Jasna
Preliminary Survey of Alternaria Toxins Reduction during Fermentation of Whole Wheat Dough
title Preliminary Survey of Alternaria Toxins Reduction during Fermentation of Whole Wheat Dough
title_full Preliminary Survey of Alternaria Toxins Reduction during Fermentation of Whole Wheat Dough
title_fullStr Preliminary Survey of Alternaria Toxins Reduction during Fermentation of Whole Wheat Dough
title_full_unstemmed Preliminary Survey of Alternaria Toxins Reduction during Fermentation of Whole Wheat Dough
title_short Preliminary Survey of Alternaria Toxins Reduction during Fermentation of Whole Wheat Dough
title_sort preliminary survey of alternaria toxins reduction during fermentation of whole wheat dough
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7074859/
https://www.ncbi.nlm.nih.gov/pubmed/32098250
http://dx.doi.org/10.3390/microorganisms8020303
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