Cargando…
Transmission of Vancomycin-Resistant Enterococci in the Hospital Setting: Uncovering the Patient–Environment Interplay
Vancomycin-resistant enterococci (VRE) are relevant nosocomial pathogens with an increasing incidence in the last decades. Their transmission is optimal in the hospital setting, as it offers two potential, large reservoirs that are closely related: susceptible patients and their environment. Here we...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7074967/ https://www.ncbi.nlm.nih.gov/pubmed/32024001 http://dx.doi.org/10.3390/microorganisms8020203 |
_version_ | 1783506946991587328 |
---|---|
author | Correa-Martinez, Carlos L. Tönnies, Hauke Froböse, Neele J. Mellmann, Alexander Kampmeier, Stefanie |
author_facet | Correa-Martinez, Carlos L. Tönnies, Hauke Froböse, Neele J. Mellmann, Alexander Kampmeier, Stefanie |
author_sort | Correa-Martinez, Carlos L. |
collection | PubMed |
description | Vancomycin-resistant enterococci (VRE) are relevant nosocomial pathogens with an increasing incidence in the last decades. Their transmission is optimal in the hospital setting, as it offers two potential, large reservoirs that are closely related: susceptible patients and their environment. Here we investigate the role of the hospital environment in the nosocomial transmission of VRE by establishing concrete links between contaminated surfaces and colonized/infected patients in outbreak and non-outbreak settings. Environmental and patient VRE isolates were collected between 2013 and 2019 and analyzed by whole-genome sequencing (WGS), subsequent multilocus sequence typing (MLST), and core genome (cg) MLST. Pairs of isolates differing in <3 alleles were rated as closely related, making a transmission likely. Fifty-three environmental VRE isolates were analyzed. MLST sequence types (ST) ST203 (50.0%), ST192 (21.3%), ST117 (17.3%), ST721 (8.8%), ST80 (2%), and ST1489 (0.7%) were detected, carrying the resistance determinants vanA (72.7%), vanB (24%), or both (3.3%). Of the 53 environmental isolates, 51 were found to form five clusters with genetically related patient isolates (n = 97 isolates). WGS confirms the role of the environment in the transmission dynamics of VRE in both the outbreak and non-outbreak settings, highlighting the importance of prevention and control of VRE spread. |
format | Online Article Text |
id | pubmed-7074967 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-70749672020-03-20 Transmission of Vancomycin-Resistant Enterococci in the Hospital Setting: Uncovering the Patient–Environment Interplay Correa-Martinez, Carlos L. Tönnies, Hauke Froböse, Neele J. Mellmann, Alexander Kampmeier, Stefanie Microorganisms Article Vancomycin-resistant enterococci (VRE) are relevant nosocomial pathogens with an increasing incidence in the last decades. Their transmission is optimal in the hospital setting, as it offers two potential, large reservoirs that are closely related: susceptible patients and their environment. Here we investigate the role of the hospital environment in the nosocomial transmission of VRE by establishing concrete links between contaminated surfaces and colonized/infected patients in outbreak and non-outbreak settings. Environmental and patient VRE isolates were collected between 2013 and 2019 and analyzed by whole-genome sequencing (WGS), subsequent multilocus sequence typing (MLST), and core genome (cg) MLST. Pairs of isolates differing in <3 alleles were rated as closely related, making a transmission likely. Fifty-three environmental VRE isolates were analyzed. MLST sequence types (ST) ST203 (50.0%), ST192 (21.3%), ST117 (17.3%), ST721 (8.8%), ST80 (2%), and ST1489 (0.7%) were detected, carrying the resistance determinants vanA (72.7%), vanB (24%), or both (3.3%). Of the 53 environmental isolates, 51 were found to form five clusters with genetically related patient isolates (n = 97 isolates). WGS confirms the role of the environment in the transmission dynamics of VRE in both the outbreak and non-outbreak settings, highlighting the importance of prevention and control of VRE spread. MDPI 2020-01-31 /pmc/articles/PMC7074967/ /pubmed/32024001 http://dx.doi.org/10.3390/microorganisms8020203 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Correa-Martinez, Carlos L. Tönnies, Hauke Froböse, Neele J. Mellmann, Alexander Kampmeier, Stefanie Transmission of Vancomycin-Resistant Enterococci in the Hospital Setting: Uncovering the Patient–Environment Interplay |
title | Transmission of Vancomycin-Resistant Enterococci in the Hospital Setting: Uncovering the Patient–Environment Interplay |
title_full | Transmission of Vancomycin-Resistant Enterococci in the Hospital Setting: Uncovering the Patient–Environment Interplay |
title_fullStr | Transmission of Vancomycin-Resistant Enterococci in the Hospital Setting: Uncovering the Patient–Environment Interplay |
title_full_unstemmed | Transmission of Vancomycin-Resistant Enterococci in the Hospital Setting: Uncovering the Patient–Environment Interplay |
title_short | Transmission of Vancomycin-Resistant Enterococci in the Hospital Setting: Uncovering the Patient–Environment Interplay |
title_sort | transmission of vancomycin-resistant enterococci in the hospital setting: uncovering the patient–environment interplay |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7074967/ https://www.ncbi.nlm.nih.gov/pubmed/32024001 http://dx.doi.org/10.3390/microorganisms8020203 |
work_keys_str_mv | AT correamartinezcarlosl transmissionofvancomycinresistantenterococciinthehospitalsettinguncoveringthepatientenvironmentinterplay AT tonnieshauke transmissionofvancomycinresistantenterococciinthehospitalsettinguncoveringthepatientenvironmentinterplay AT froboseneelej transmissionofvancomycinresistantenterococciinthehospitalsettinguncoveringthepatientenvironmentinterplay AT mellmannalexander transmissionofvancomycinresistantenterococciinthehospitalsettinguncoveringthepatientenvironmentinterplay AT kampmeierstefanie transmissionofvancomycinresistantenterococciinthehospitalsettinguncoveringthepatientenvironmentinterplay |