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Carotenoids from Cyanobacteria: A Biotechnological Approach for the Topical Treatment of Psoriasis

In this study, five cyanobacteria strains (Alkalinema aff. pantanalense LEGE15481, Cyanobium gracile LEGE12431, Nodosilinea (Leptolyngbya) antarctica LEGE13457, Cuspidothrix issatschenkoi LEGE03282 and Leptolyngbya-like sp. LEGE13412) from the Blue Biotechnology and Ecotoxicology Culture Collection...

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Autores principales: Lopes, Graciliana, Clarinha, Duarte, Vasconcelos, Vitor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7074968/
https://www.ncbi.nlm.nih.gov/pubmed/32098255
http://dx.doi.org/10.3390/microorganisms8020302
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author Lopes, Graciliana
Clarinha, Duarte
Vasconcelos, Vitor
author_facet Lopes, Graciliana
Clarinha, Duarte
Vasconcelos, Vitor
author_sort Lopes, Graciliana
collection PubMed
description In this study, five cyanobacteria strains (Alkalinema aff. pantanalense LEGE15481, Cyanobium gracile LEGE12431, Nodosilinea (Leptolyngbya) antarctica LEGE13457, Cuspidothrix issatschenkoi LEGE03282 and Leptolyngbya-like sp. LEGE13412) from the Blue Biotechnology and Ecotoxicology Culture Collection (LEGE CC) of CIIMAR were explored for their biotechnological potential in the treatment of psoriasis. Different extracts were characterized for their pigment profile by HPLC-PDA. The antioxidant potential of the extracts was assessed against the superoxide anion radical (O(2)(•-)). Their anti-inflammatory and antiproliferative potential was assessed in vitro using the macrophages RAW 264.7 and the human keratinocytes HaCaT as cell-line models, respectively. Terrestrial and freshwater strains presented the highest carotenoid content (33193−63926 μg/g dry extract), with all-trans-β-carotene, zeaxanthin, echinenone and lutein derivatives being the most abundant carotenoids. Acetone was the most effective solvent for pigment extraction. The acetone extracts presented the lowest IC(50) values (0.29−0.38 mg dry extract/mL) regarding O(2)(•-) scavenging, and revealed anti-inflammatory potential, with N. antarctica LEGE13457, A. pantanalense LEGE15481 and Leptolyngbya-like sp. LEGE13412 reducing the nitric oxide (NO) in RAW 264.7 cell culture medium in about 25% (p < 0.05). With the exception of A. pantanalense LEGE15481, all the extracts significantly reduced keratinocyte proliferation (p < 0.05), demonstrating a selective toxicity among the different cell lines. Overall, Leptolyngbya-like sp. LEGE13412 and N. antarctica LEGE13457 seem promising for further exploitation in the framework of psoriasis, due to their antioxidant, anti-inflammatory and antiproliferative potential.
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spelling pubmed-70749682020-03-20 Carotenoids from Cyanobacteria: A Biotechnological Approach for the Topical Treatment of Psoriasis Lopes, Graciliana Clarinha, Duarte Vasconcelos, Vitor Microorganisms Article In this study, five cyanobacteria strains (Alkalinema aff. pantanalense LEGE15481, Cyanobium gracile LEGE12431, Nodosilinea (Leptolyngbya) antarctica LEGE13457, Cuspidothrix issatschenkoi LEGE03282 and Leptolyngbya-like sp. LEGE13412) from the Blue Biotechnology and Ecotoxicology Culture Collection (LEGE CC) of CIIMAR were explored for their biotechnological potential in the treatment of psoriasis. Different extracts were characterized for their pigment profile by HPLC-PDA. The antioxidant potential of the extracts was assessed against the superoxide anion radical (O(2)(•-)). Their anti-inflammatory and antiproliferative potential was assessed in vitro using the macrophages RAW 264.7 and the human keratinocytes HaCaT as cell-line models, respectively. Terrestrial and freshwater strains presented the highest carotenoid content (33193−63926 μg/g dry extract), with all-trans-β-carotene, zeaxanthin, echinenone and lutein derivatives being the most abundant carotenoids. Acetone was the most effective solvent for pigment extraction. The acetone extracts presented the lowest IC(50) values (0.29−0.38 mg dry extract/mL) regarding O(2)(•-) scavenging, and revealed anti-inflammatory potential, with N. antarctica LEGE13457, A. pantanalense LEGE15481 and Leptolyngbya-like sp. LEGE13412 reducing the nitric oxide (NO) in RAW 264.7 cell culture medium in about 25% (p < 0.05). With the exception of A. pantanalense LEGE15481, all the extracts significantly reduced keratinocyte proliferation (p < 0.05), demonstrating a selective toxicity among the different cell lines. Overall, Leptolyngbya-like sp. LEGE13412 and N. antarctica LEGE13457 seem promising for further exploitation in the framework of psoriasis, due to their antioxidant, anti-inflammatory and antiproliferative potential. MDPI 2020-02-21 /pmc/articles/PMC7074968/ /pubmed/32098255 http://dx.doi.org/10.3390/microorganisms8020302 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lopes, Graciliana
Clarinha, Duarte
Vasconcelos, Vitor
Carotenoids from Cyanobacteria: A Biotechnological Approach for the Topical Treatment of Psoriasis
title Carotenoids from Cyanobacteria: A Biotechnological Approach for the Topical Treatment of Psoriasis
title_full Carotenoids from Cyanobacteria: A Biotechnological Approach for the Topical Treatment of Psoriasis
title_fullStr Carotenoids from Cyanobacteria: A Biotechnological Approach for the Topical Treatment of Psoriasis
title_full_unstemmed Carotenoids from Cyanobacteria: A Biotechnological Approach for the Topical Treatment of Psoriasis
title_short Carotenoids from Cyanobacteria: A Biotechnological Approach for the Topical Treatment of Psoriasis
title_sort carotenoids from cyanobacteria: a biotechnological approach for the topical treatment of psoriasis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7074968/
https://www.ncbi.nlm.nih.gov/pubmed/32098255
http://dx.doi.org/10.3390/microorganisms8020302
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