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Membrane expression of the estrogen receptor ERα is required for intercellular communications in the mammary epithelium

17β-Estradiol induces the postnatal development of mammary gland and influences breast carcinogenesis by binding to the estrogen receptor ERα. ERα acts as a transcription factor but also elicits rapid signaling through a fraction of ERα expressed at the membrane. Here, we have used the C451A-ERα mou...

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Autores principales: Gagniac, Laurine, Rusidzé, Mariam, Boudou, Frederic, Cagnet, Stephanie, Adlanmerini, Marine, Jeannot, Pauline, Gaide, Nicolas, Giton, Frank, Besson, Arnaud, Weyl, Ariane, Gourdy, Pierre, Raymond-Letron, Isabelle, Arnal, Jean-Francois, Brisken, Cathrin, Lenfant, Francoise
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists Ltd 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7075076/
https://www.ncbi.nlm.nih.gov/pubmed/32098763
http://dx.doi.org/10.1242/dev.182303
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author Gagniac, Laurine
Rusidzé, Mariam
Boudou, Frederic
Cagnet, Stephanie
Adlanmerini, Marine
Jeannot, Pauline
Gaide, Nicolas
Giton, Frank
Besson, Arnaud
Weyl, Ariane
Gourdy, Pierre
Raymond-Letron, Isabelle
Arnal, Jean-Francois
Brisken, Cathrin
Lenfant, Francoise
author_facet Gagniac, Laurine
Rusidzé, Mariam
Boudou, Frederic
Cagnet, Stephanie
Adlanmerini, Marine
Jeannot, Pauline
Gaide, Nicolas
Giton, Frank
Besson, Arnaud
Weyl, Ariane
Gourdy, Pierre
Raymond-Letron, Isabelle
Arnal, Jean-Francois
Brisken, Cathrin
Lenfant, Francoise
author_sort Gagniac, Laurine
collection PubMed
description 17β-Estradiol induces the postnatal development of mammary gland and influences breast carcinogenesis by binding to the estrogen receptor ERα. ERα acts as a transcription factor but also elicits rapid signaling through a fraction of ERα expressed at the membrane. Here, we have used the C451A-ERα mouse model mutated for the palmitoylation site to understand how ERα membrane signaling affects mammary gland development. Although the overall structure of physiological mammary gland development is slightly affected, both epithelial fragments and basal cells isolated from C451A-ERα mammary glands failed to grow when engrafted into cleared wild-type fat pads, even in pregnant hosts. Similarly, basal cells purified from hormone-stimulated ovariectomized C451A-ERα mice did not produce normal outgrowths. Ex vivo, C451A-ERα basal cells displayed reduced matrix degradation capacities, suggesting altered migration properties. More importantly, C451A-ERα basal cells recovered in vivo repopulating ability when co-transplanted with wild-type luminal cells and specifically with ERα-positive luminal cells. Transcriptional profiling identified crucial paracrine luminal-to-basal signals. Altogether, our findings uncover an important role for membrane ERα expression in promoting intercellular communications that are essential for mammary gland development.
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spelling pubmed-70750762020-04-08 Membrane expression of the estrogen receptor ERα is required for intercellular communications in the mammary epithelium Gagniac, Laurine Rusidzé, Mariam Boudou, Frederic Cagnet, Stephanie Adlanmerini, Marine Jeannot, Pauline Gaide, Nicolas Giton, Frank Besson, Arnaud Weyl, Ariane Gourdy, Pierre Raymond-Letron, Isabelle Arnal, Jean-Francois Brisken, Cathrin Lenfant, Francoise Development Stem Cells and Regeneration 17β-Estradiol induces the postnatal development of mammary gland and influences breast carcinogenesis by binding to the estrogen receptor ERα. ERα acts as a transcription factor but also elicits rapid signaling through a fraction of ERα expressed at the membrane. Here, we have used the C451A-ERα mouse model mutated for the palmitoylation site to understand how ERα membrane signaling affects mammary gland development. Although the overall structure of physiological mammary gland development is slightly affected, both epithelial fragments and basal cells isolated from C451A-ERα mammary glands failed to grow when engrafted into cleared wild-type fat pads, even in pregnant hosts. Similarly, basal cells purified from hormone-stimulated ovariectomized C451A-ERα mice did not produce normal outgrowths. Ex vivo, C451A-ERα basal cells displayed reduced matrix degradation capacities, suggesting altered migration properties. More importantly, C451A-ERα basal cells recovered in vivo repopulating ability when co-transplanted with wild-type luminal cells and specifically with ERα-positive luminal cells. Transcriptional profiling identified crucial paracrine luminal-to-basal signals. Altogether, our findings uncover an important role for membrane ERα expression in promoting intercellular communications that are essential for mammary gland development. The Company of Biologists Ltd 2020-03-11 /pmc/articles/PMC7075076/ /pubmed/32098763 http://dx.doi.org/10.1242/dev.182303 Text en © 2020. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/4.0This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
spellingShingle Stem Cells and Regeneration
Gagniac, Laurine
Rusidzé, Mariam
Boudou, Frederic
Cagnet, Stephanie
Adlanmerini, Marine
Jeannot, Pauline
Gaide, Nicolas
Giton, Frank
Besson, Arnaud
Weyl, Ariane
Gourdy, Pierre
Raymond-Letron, Isabelle
Arnal, Jean-Francois
Brisken, Cathrin
Lenfant, Francoise
Membrane expression of the estrogen receptor ERα is required for intercellular communications in the mammary epithelium
title Membrane expression of the estrogen receptor ERα is required for intercellular communications in the mammary epithelium
title_full Membrane expression of the estrogen receptor ERα is required for intercellular communications in the mammary epithelium
title_fullStr Membrane expression of the estrogen receptor ERα is required for intercellular communications in the mammary epithelium
title_full_unstemmed Membrane expression of the estrogen receptor ERα is required for intercellular communications in the mammary epithelium
title_short Membrane expression of the estrogen receptor ERα is required for intercellular communications in the mammary epithelium
title_sort membrane expression of the estrogen receptor erα is required for intercellular communications in the mammary epithelium
topic Stem Cells and Regeneration
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7075076/
https://www.ncbi.nlm.nih.gov/pubmed/32098763
http://dx.doi.org/10.1242/dev.182303
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