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Membrane expression of the estrogen receptor ERα is required for intercellular communications in the mammary epithelium
17β-Estradiol induces the postnatal development of mammary gland and influences breast carcinogenesis by binding to the estrogen receptor ERα. ERα acts as a transcription factor but also elicits rapid signaling through a fraction of ERα expressed at the membrane. Here, we have used the C451A-ERα mou...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Company of Biologists Ltd
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7075076/ https://www.ncbi.nlm.nih.gov/pubmed/32098763 http://dx.doi.org/10.1242/dev.182303 |
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author | Gagniac, Laurine Rusidzé, Mariam Boudou, Frederic Cagnet, Stephanie Adlanmerini, Marine Jeannot, Pauline Gaide, Nicolas Giton, Frank Besson, Arnaud Weyl, Ariane Gourdy, Pierre Raymond-Letron, Isabelle Arnal, Jean-Francois Brisken, Cathrin Lenfant, Francoise |
author_facet | Gagniac, Laurine Rusidzé, Mariam Boudou, Frederic Cagnet, Stephanie Adlanmerini, Marine Jeannot, Pauline Gaide, Nicolas Giton, Frank Besson, Arnaud Weyl, Ariane Gourdy, Pierre Raymond-Letron, Isabelle Arnal, Jean-Francois Brisken, Cathrin Lenfant, Francoise |
author_sort | Gagniac, Laurine |
collection | PubMed |
description | 17β-Estradiol induces the postnatal development of mammary gland and influences breast carcinogenesis by binding to the estrogen receptor ERα. ERα acts as a transcription factor but also elicits rapid signaling through a fraction of ERα expressed at the membrane. Here, we have used the C451A-ERα mouse model mutated for the palmitoylation site to understand how ERα membrane signaling affects mammary gland development. Although the overall structure of physiological mammary gland development is slightly affected, both epithelial fragments and basal cells isolated from C451A-ERα mammary glands failed to grow when engrafted into cleared wild-type fat pads, even in pregnant hosts. Similarly, basal cells purified from hormone-stimulated ovariectomized C451A-ERα mice did not produce normal outgrowths. Ex vivo, C451A-ERα basal cells displayed reduced matrix degradation capacities, suggesting altered migration properties. More importantly, C451A-ERα basal cells recovered in vivo repopulating ability when co-transplanted with wild-type luminal cells and specifically with ERα-positive luminal cells. Transcriptional profiling identified crucial paracrine luminal-to-basal signals. Altogether, our findings uncover an important role for membrane ERα expression in promoting intercellular communications that are essential for mammary gland development. |
format | Online Article Text |
id | pubmed-7075076 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | The Company of Biologists Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-70750762020-04-08 Membrane expression of the estrogen receptor ERα is required for intercellular communications in the mammary epithelium Gagniac, Laurine Rusidzé, Mariam Boudou, Frederic Cagnet, Stephanie Adlanmerini, Marine Jeannot, Pauline Gaide, Nicolas Giton, Frank Besson, Arnaud Weyl, Ariane Gourdy, Pierre Raymond-Letron, Isabelle Arnal, Jean-Francois Brisken, Cathrin Lenfant, Francoise Development Stem Cells and Regeneration 17β-Estradiol induces the postnatal development of mammary gland and influences breast carcinogenesis by binding to the estrogen receptor ERα. ERα acts as a transcription factor but also elicits rapid signaling through a fraction of ERα expressed at the membrane. Here, we have used the C451A-ERα mouse model mutated for the palmitoylation site to understand how ERα membrane signaling affects mammary gland development. Although the overall structure of physiological mammary gland development is slightly affected, both epithelial fragments and basal cells isolated from C451A-ERα mammary glands failed to grow when engrafted into cleared wild-type fat pads, even in pregnant hosts. Similarly, basal cells purified from hormone-stimulated ovariectomized C451A-ERα mice did not produce normal outgrowths. Ex vivo, C451A-ERα basal cells displayed reduced matrix degradation capacities, suggesting altered migration properties. More importantly, C451A-ERα basal cells recovered in vivo repopulating ability when co-transplanted with wild-type luminal cells and specifically with ERα-positive luminal cells. Transcriptional profiling identified crucial paracrine luminal-to-basal signals. Altogether, our findings uncover an important role for membrane ERα expression in promoting intercellular communications that are essential for mammary gland development. The Company of Biologists Ltd 2020-03-11 /pmc/articles/PMC7075076/ /pubmed/32098763 http://dx.doi.org/10.1242/dev.182303 Text en © 2020. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/4.0This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. |
spellingShingle | Stem Cells and Regeneration Gagniac, Laurine Rusidzé, Mariam Boudou, Frederic Cagnet, Stephanie Adlanmerini, Marine Jeannot, Pauline Gaide, Nicolas Giton, Frank Besson, Arnaud Weyl, Ariane Gourdy, Pierre Raymond-Letron, Isabelle Arnal, Jean-Francois Brisken, Cathrin Lenfant, Francoise Membrane expression of the estrogen receptor ERα is required for intercellular communications in the mammary epithelium |
title | Membrane expression of the estrogen receptor ERα is required for intercellular communications in the mammary epithelium |
title_full | Membrane expression of the estrogen receptor ERα is required for intercellular communications in the mammary epithelium |
title_fullStr | Membrane expression of the estrogen receptor ERα is required for intercellular communications in the mammary epithelium |
title_full_unstemmed | Membrane expression of the estrogen receptor ERα is required for intercellular communications in the mammary epithelium |
title_short | Membrane expression of the estrogen receptor ERα is required for intercellular communications in the mammary epithelium |
title_sort | membrane expression of the estrogen receptor erα is required for intercellular communications in the mammary epithelium |
topic | Stem Cells and Regeneration |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7075076/ https://www.ncbi.nlm.nih.gov/pubmed/32098763 http://dx.doi.org/10.1242/dev.182303 |
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