Cargando…

Removal of Diclofenac, Paracetamol, and Carbamazepine from Model Aqueous Solutions by Magnetic Sol–Gel Encapsulated Horseradish Peroxidase and Lignin Peroxidase Composites

Sustainable and green synthesis of nanocomposites for degradation of pharmaceuticals was developed via immobilization and stabilization of the biological strong oxidizing agents, peroxidase enzymes, on a solid support. Sol–gel encapsulated enzyme composites were characterized using electron microsco...

Descripción completa

Detalles Bibliográficos
Autores principales: Pylypchuk, Ievgen V., Daniel, Geoffrey, Kessler, Vadim G., Seisenbaeva, Gulaim A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7075194/
https://www.ncbi.nlm.nih.gov/pubmed/32046049
http://dx.doi.org/10.3390/nano10020282
_version_ 1783506990702526464
author Pylypchuk, Ievgen V.
Daniel, Geoffrey
Kessler, Vadim G.
Seisenbaeva, Gulaim A.
author_facet Pylypchuk, Ievgen V.
Daniel, Geoffrey
Kessler, Vadim G.
Seisenbaeva, Gulaim A.
author_sort Pylypchuk, Ievgen V.
collection PubMed
description Sustainable and green synthesis of nanocomposites for degradation of pharmaceuticals was developed via immobilization and stabilization of the biological strong oxidizing agents, peroxidase enzymes, on a solid support. Sol–gel encapsulated enzyme composites were characterized using electron microscopy (TEM, SEM), atomic force microscopy, FTIR spectroscopy, and thermogravimetric analysis. Horseradish peroxidase (HRP) and lignin peroxidase (LiP) were adsorbed onto magnetite nanoparticles and sol–gel encapsulated in a surface silica layer. Encapsulation enhanced the stability of the biocatalysts over time and thermal stability. The biocatalysts showed appreciable selectivity in oxidation of the organic drinking water pollutants diclofenac, carbamazepine, and paracetamol with improved activity being pharmaceutical specific for each enzyme. In particular, sol–gel encapsulated LiP- and HRP-based nanocomposites were active over 20 consecutive cycles for 20 days at 55 °C (24 h/cycle). The stability of the sol–gel encapsulated catalysts in acidic medium was also improved compared to native enzymes. Carbamazepine and diclofenac were degraded to 68% and 64% by sol–gel LiP composites respectively at pH 5 under elevated temperature. Total destruction of carbamazepine and diclofenac was achieved at pH 3 (55 °C) within 3 days, in the case of both immobilized HRP and LiP. Using NMR spectroscopy, characterization of the drug decomposition products, and decomposition pathways by the peroxidase enzymes suggested.
format Online
Article
Text
id pubmed-7075194
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-70751942020-03-20 Removal of Diclofenac, Paracetamol, and Carbamazepine from Model Aqueous Solutions by Magnetic Sol–Gel Encapsulated Horseradish Peroxidase and Lignin Peroxidase Composites Pylypchuk, Ievgen V. Daniel, Geoffrey Kessler, Vadim G. Seisenbaeva, Gulaim A. Nanomaterials (Basel) Article Sustainable and green synthesis of nanocomposites for degradation of pharmaceuticals was developed via immobilization and stabilization of the biological strong oxidizing agents, peroxidase enzymes, on a solid support. Sol–gel encapsulated enzyme composites were characterized using electron microscopy (TEM, SEM), atomic force microscopy, FTIR spectroscopy, and thermogravimetric analysis. Horseradish peroxidase (HRP) and lignin peroxidase (LiP) were adsorbed onto magnetite nanoparticles and sol–gel encapsulated in a surface silica layer. Encapsulation enhanced the stability of the biocatalysts over time and thermal stability. The biocatalysts showed appreciable selectivity in oxidation of the organic drinking water pollutants diclofenac, carbamazepine, and paracetamol with improved activity being pharmaceutical specific for each enzyme. In particular, sol–gel encapsulated LiP- and HRP-based nanocomposites were active over 20 consecutive cycles for 20 days at 55 °C (24 h/cycle). The stability of the sol–gel encapsulated catalysts in acidic medium was also improved compared to native enzymes. Carbamazepine and diclofenac were degraded to 68% and 64% by sol–gel LiP composites respectively at pH 5 under elevated temperature. Total destruction of carbamazepine and diclofenac was achieved at pH 3 (55 °C) within 3 days, in the case of both immobilized HRP and LiP. Using NMR spectroscopy, characterization of the drug decomposition products, and decomposition pathways by the peroxidase enzymes suggested. MDPI 2020-02-07 /pmc/articles/PMC7075194/ /pubmed/32046049 http://dx.doi.org/10.3390/nano10020282 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pylypchuk, Ievgen V.
Daniel, Geoffrey
Kessler, Vadim G.
Seisenbaeva, Gulaim A.
Removal of Diclofenac, Paracetamol, and Carbamazepine from Model Aqueous Solutions by Magnetic Sol–Gel Encapsulated Horseradish Peroxidase and Lignin Peroxidase Composites
title Removal of Diclofenac, Paracetamol, and Carbamazepine from Model Aqueous Solutions by Magnetic Sol–Gel Encapsulated Horseradish Peroxidase and Lignin Peroxidase Composites
title_full Removal of Diclofenac, Paracetamol, and Carbamazepine from Model Aqueous Solutions by Magnetic Sol–Gel Encapsulated Horseradish Peroxidase and Lignin Peroxidase Composites
title_fullStr Removal of Diclofenac, Paracetamol, and Carbamazepine from Model Aqueous Solutions by Magnetic Sol–Gel Encapsulated Horseradish Peroxidase and Lignin Peroxidase Composites
title_full_unstemmed Removal of Diclofenac, Paracetamol, and Carbamazepine from Model Aqueous Solutions by Magnetic Sol–Gel Encapsulated Horseradish Peroxidase and Lignin Peroxidase Composites
title_short Removal of Diclofenac, Paracetamol, and Carbamazepine from Model Aqueous Solutions by Magnetic Sol–Gel Encapsulated Horseradish Peroxidase and Lignin Peroxidase Composites
title_sort removal of diclofenac, paracetamol, and carbamazepine from model aqueous solutions by magnetic sol–gel encapsulated horseradish peroxidase and lignin peroxidase composites
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7075194/
https://www.ncbi.nlm.nih.gov/pubmed/32046049
http://dx.doi.org/10.3390/nano10020282
work_keys_str_mv AT pylypchukievgenv removalofdiclofenacparacetamolandcarbamazepinefrommodelaqueoussolutionsbymagneticsolgelencapsulatedhorseradishperoxidaseandligninperoxidasecomposites
AT danielgeoffrey removalofdiclofenacparacetamolandcarbamazepinefrommodelaqueoussolutionsbymagneticsolgelencapsulatedhorseradishperoxidaseandligninperoxidasecomposites
AT kesslervadimg removalofdiclofenacparacetamolandcarbamazepinefrommodelaqueoussolutionsbymagneticsolgelencapsulatedhorseradishperoxidaseandligninperoxidasecomposites
AT seisenbaevagulaima removalofdiclofenacparacetamolandcarbamazepinefrommodelaqueoussolutionsbymagneticsolgelencapsulatedhorseradishperoxidaseandligninperoxidasecomposites