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Ex Vivo Permeation of Carprofen Vehiculated by PLGA Nanoparticles through Porcine Mucous Membranes and Ophthalmic Tissues

(1) Background: Carprofen (CP), 2-(6-chlorocarbazole) propionic acid, is used as an anti-inflammatory, analgesic and anti-pyretic agent and it belongs to the family of non-steroidal anti-inflammatory drugs (NSAIDs). CP has some adverse reactions in systemic administration; for this reason, topical a...

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Autores principales: Gómez-Segura, Lídia, Parra, Alexander, Calpena-Campmany, Ana Cristina, Gimeno, Álvaro, Gómez de Aranda, Immaculada, Boix-Montañes, Antonio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7075292/
https://www.ncbi.nlm.nih.gov/pubmed/32085577
http://dx.doi.org/10.3390/nano10020355
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author Gómez-Segura, Lídia
Parra, Alexander
Calpena-Campmany, Ana Cristina
Gimeno, Álvaro
Gómez de Aranda, Immaculada
Boix-Montañes, Antonio
author_facet Gómez-Segura, Lídia
Parra, Alexander
Calpena-Campmany, Ana Cristina
Gimeno, Álvaro
Gómez de Aranda, Immaculada
Boix-Montañes, Antonio
author_sort Gómez-Segura, Lídia
collection PubMed
description (1) Background: Carprofen (CP), 2-(6-chlorocarbazole) propionic acid, is used as an anti-inflammatory, analgesic and anti-pyretic agent and it belongs to the family of non-steroidal anti-inflammatory drugs (NSAIDs). CP has some adverse reactions in systemic administration; for this reason, topical administration with CP nanoparticles (CP-NPs) can be an optimal alternative. The main objective of this work is the investigation of ex vivo permeation of CP through different types of porcine mucous membranes (buccal, sublingual and vaginal) and ophthalmic tissues (cornea, sclera and conjunctiva) to compare the influence of CP-NPs formulation over a CP solution (CP-Solution). (2) Methods: The ex vivo permeation profiles were evaluated using Franz diffusion cells. Furthermore, in vivo studies were performed to verify that the formulations did not affect the cell structure and to establish the amount retained (Qr) in the tissues. (3) Results: Permeation of CP-NPs is more effective in terms of drug retention in almost all tissues (with the exception of sclera and sublingual). In vivo studies show that neither of the two formulations affects tissue structure, so both formulations are safe. (4) Conclusions: It was concluded that CP-NPs may be a useful tool for the topical treatment of local inflammation in veterinary and human medicine.
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spelling pubmed-70752922020-03-20 Ex Vivo Permeation of Carprofen Vehiculated by PLGA Nanoparticles through Porcine Mucous Membranes and Ophthalmic Tissues Gómez-Segura, Lídia Parra, Alexander Calpena-Campmany, Ana Cristina Gimeno, Álvaro Gómez de Aranda, Immaculada Boix-Montañes, Antonio Nanomaterials (Basel) Article (1) Background: Carprofen (CP), 2-(6-chlorocarbazole) propionic acid, is used as an anti-inflammatory, analgesic and anti-pyretic agent and it belongs to the family of non-steroidal anti-inflammatory drugs (NSAIDs). CP has some adverse reactions in systemic administration; for this reason, topical administration with CP nanoparticles (CP-NPs) can be an optimal alternative. The main objective of this work is the investigation of ex vivo permeation of CP through different types of porcine mucous membranes (buccal, sublingual and vaginal) and ophthalmic tissues (cornea, sclera and conjunctiva) to compare the influence of CP-NPs formulation over a CP solution (CP-Solution). (2) Methods: The ex vivo permeation profiles were evaluated using Franz diffusion cells. Furthermore, in vivo studies were performed to verify that the formulations did not affect the cell structure and to establish the amount retained (Qr) in the tissues. (3) Results: Permeation of CP-NPs is more effective in terms of drug retention in almost all tissues (with the exception of sclera and sublingual). In vivo studies show that neither of the two formulations affects tissue structure, so both formulations are safe. (4) Conclusions: It was concluded that CP-NPs may be a useful tool for the topical treatment of local inflammation in veterinary and human medicine. MDPI 2020-02-18 /pmc/articles/PMC7075292/ /pubmed/32085577 http://dx.doi.org/10.3390/nano10020355 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gómez-Segura, Lídia
Parra, Alexander
Calpena-Campmany, Ana Cristina
Gimeno, Álvaro
Gómez de Aranda, Immaculada
Boix-Montañes, Antonio
Ex Vivo Permeation of Carprofen Vehiculated by PLGA Nanoparticles through Porcine Mucous Membranes and Ophthalmic Tissues
title Ex Vivo Permeation of Carprofen Vehiculated by PLGA Nanoparticles through Porcine Mucous Membranes and Ophthalmic Tissues
title_full Ex Vivo Permeation of Carprofen Vehiculated by PLGA Nanoparticles through Porcine Mucous Membranes and Ophthalmic Tissues
title_fullStr Ex Vivo Permeation of Carprofen Vehiculated by PLGA Nanoparticles through Porcine Mucous Membranes and Ophthalmic Tissues
title_full_unstemmed Ex Vivo Permeation of Carprofen Vehiculated by PLGA Nanoparticles through Porcine Mucous Membranes and Ophthalmic Tissues
title_short Ex Vivo Permeation of Carprofen Vehiculated by PLGA Nanoparticles through Porcine Mucous Membranes and Ophthalmic Tissues
title_sort ex vivo permeation of carprofen vehiculated by plga nanoparticles through porcine mucous membranes and ophthalmic tissues
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7075292/
https://www.ncbi.nlm.nih.gov/pubmed/32085577
http://dx.doi.org/10.3390/nano10020355
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