Cargando…

Potential Role of Soluble Metal Impurities in the Acute Lung Inflammogenicity of Multi-Walled Carbon Nanotubes

Multi-walled carbon nanotubes (MWCNTs) have variable metal impurities, but little is known about the impact of soluble metal impurities on the toxicity of MWCNTs. Here, we evaluated the role of soluble metal impurities to the acute inflammogenic potential of MWCNTs, using five types of high purity M...

Descripción completa

Detalles Bibliográficos
Autores principales: Lee, Dong-Keun, Jeon, Soyeon, Jeong, Jiyoung, Yu, Il Je, Song, Kyung Seuk, Kang, Aeyeon, Yun, Wan Soo, Kim, Jong Sung, Cho, Wan-Seob
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7075329/
https://www.ncbi.nlm.nih.gov/pubmed/32098206
http://dx.doi.org/10.3390/nano10020379
_version_ 1783507021382811648
author Lee, Dong-Keun
Jeon, Soyeon
Jeong, Jiyoung
Yu, Il Je
Song, Kyung Seuk
Kang, Aeyeon
Yun, Wan Soo
Kim, Jong Sung
Cho, Wan-Seob
author_facet Lee, Dong-Keun
Jeon, Soyeon
Jeong, Jiyoung
Yu, Il Je
Song, Kyung Seuk
Kang, Aeyeon
Yun, Wan Soo
Kim, Jong Sung
Cho, Wan-Seob
author_sort Lee, Dong-Keun
collection PubMed
description Multi-walled carbon nanotubes (MWCNTs) have variable metal impurities, but little is known about the impact of soluble metal impurities on the toxicity of MWCNTs. Here, we evaluated the role of soluble metal impurities to the acute inflammogenic potential of MWCNTs, using five types of high purity MWCNTs (>95%). MWCNTs and their soluble fractions collected at 24 h after incubation in phosphate-buffered saline showed diverse metal impurities with variable concentrations. The fiber-free soluble fractions produced variable levels of reactive oxygen species (ROS), and the iron level was the key determinant for ROS production. The acute inflammation at 24 h after intratracheal instillation of MWCNTs to rats at 0.19, 0.63, and 1.91 mg MWCNT/kg body weight (bw) or fiber-free supernatants from MWCNT suspensions at 1.91 and 7.64 mg MWCNT/kg bw showed that the number of granulocytes, a marker for acute inflammation, was significantly increased with a good dose-dependency. The correlation study showed that neither the levels of iron nor the ROS generation potential of the soluble fractions showed any correlations with the inflammogenic potential. However, the total concentration of transition metals in the soluble fractions showed a good correlation with the acute lung inflammogenic potential. These results implied that metal impurities, especially transitional metals, can contribute to the acute inflammogenic potential of MWCNTs, although the major parameter for the toxicity of MWCNTs is size and shape.
format Online
Article
Text
id pubmed-7075329
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-70753292020-03-20 Potential Role of Soluble Metal Impurities in the Acute Lung Inflammogenicity of Multi-Walled Carbon Nanotubes Lee, Dong-Keun Jeon, Soyeon Jeong, Jiyoung Yu, Il Je Song, Kyung Seuk Kang, Aeyeon Yun, Wan Soo Kim, Jong Sung Cho, Wan-Seob Nanomaterials (Basel) Article Multi-walled carbon nanotubes (MWCNTs) have variable metal impurities, but little is known about the impact of soluble metal impurities on the toxicity of MWCNTs. Here, we evaluated the role of soluble metal impurities to the acute inflammogenic potential of MWCNTs, using five types of high purity MWCNTs (>95%). MWCNTs and their soluble fractions collected at 24 h after incubation in phosphate-buffered saline showed diverse metal impurities with variable concentrations. The fiber-free soluble fractions produced variable levels of reactive oxygen species (ROS), and the iron level was the key determinant for ROS production. The acute inflammation at 24 h after intratracheal instillation of MWCNTs to rats at 0.19, 0.63, and 1.91 mg MWCNT/kg body weight (bw) or fiber-free supernatants from MWCNT suspensions at 1.91 and 7.64 mg MWCNT/kg bw showed that the number of granulocytes, a marker for acute inflammation, was significantly increased with a good dose-dependency. The correlation study showed that neither the levels of iron nor the ROS generation potential of the soluble fractions showed any correlations with the inflammogenic potential. However, the total concentration of transition metals in the soluble fractions showed a good correlation with the acute lung inflammogenic potential. These results implied that metal impurities, especially transitional metals, can contribute to the acute inflammogenic potential of MWCNTs, although the major parameter for the toxicity of MWCNTs is size and shape. MDPI 2020-02-21 /pmc/articles/PMC7075329/ /pubmed/32098206 http://dx.doi.org/10.3390/nano10020379 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lee, Dong-Keun
Jeon, Soyeon
Jeong, Jiyoung
Yu, Il Je
Song, Kyung Seuk
Kang, Aeyeon
Yun, Wan Soo
Kim, Jong Sung
Cho, Wan-Seob
Potential Role of Soluble Metal Impurities in the Acute Lung Inflammogenicity of Multi-Walled Carbon Nanotubes
title Potential Role of Soluble Metal Impurities in the Acute Lung Inflammogenicity of Multi-Walled Carbon Nanotubes
title_full Potential Role of Soluble Metal Impurities in the Acute Lung Inflammogenicity of Multi-Walled Carbon Nanotubes
title_fullStr Potential Role of Soluble Metal Impurities in the Acute Lung Inflammogenicity of Multi-Walled Carbon Nanotubes
title_full_unstemmed Potential Role of Soluble Metal Impurities in the Acute Lung Inflammogenicity of Multi-Walled Carbon Nanotubes
title_short Potential Role of Soluble Metal Impurities in the Acute Lung Inflammogenicity of Multi-Walled Carbon Nanotubes
title_sort potential role of soluble metal impurities in the acute lung inflammogenicity of multi-walled carbon nanotubes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7075329/
https://www.ncbi.nlm.nih.gov/pubmed/32098206
http://dx.doi.org/10.3390/nano10020379
work_keys_str_mv AT leedongkeun potentialroleofsolublemetalimpuritiesintheacutelunginflammogenicityofmultiwalledcarbonnanotubes
AT jeonsoyeon potentialroleofsolublemetalimpuritiesintheacutelunginflammogenicityofmultiwalledcarbonnanotubes
AT jeongjiyoung potentialroleofsolublemetalimpuritiesintheacutelunginflammogenicityofmultiwalledcarbonnanotubes
AT yuilje potentialroleofsolublemetalimpuritiesintheacutelunginflammogenicityofmultiwalledcarbonnanotubes
AT songkyungseuk potentialroleofsolublemetalimpuritiesintheacutelunginflammogenicityofmultiwalledcarbonnanotubes
AT kangaeyeon potentialroleofsolublemetalimpuritiesintheacutelunginflammogenicityofmultiwalledcarbonnanotubes
AT yunwansoo potentialroleofsolublemetalimpuritiesintheacutelunginflammogenicityofmultiwalledcarbonnanotubes
AT kimjongsung potentialroleofsolublemetalimpuritiesintheacutelunginflammogenicityofmultiwalledcarbonnanotubes
AT chowanseob potentialroleofsolublemetalimpuritiesintheacutelunginflammogenicityofmultiwalledcarbonnanotubes