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Non-invasive evaluation of the equine gastrointestinal mucosal transcriptome

Evaluating the health and function of the gastrointestinal tract can be challenging in all species, but is especially difficult in horses due to their size and length of the gastrointestinal (GI) tract. Isolation of mRNA of cells exfoliated from the GI mucosa into feces (i.e., the exfoliome) offers...

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Autores principales: Coleman, Michelle C., Whitfield-Cargile, Canaan, Cohen, Noah D., Goldsby, Jennifer L., Davidson, Laurie, Chamoun-Emanuelli, Ana M., Ivanov, Ivan, Eades, Susan, Ing, Nancy, Chapkin, Robert S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7075554/
https://www.ncbi.nlm.nih.gov/pubmed/32176710
http://dx.doi.org/10.1371/journal.pone.0229797
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author Coleman, Michelle C.
Whitfield-Cargile, Canaan
Cohen, Noah D.
Goldsby, Jennifer L.
Davidson, Laurie
Chamoun-Emanuelli, Ana M.
Ivanov, Ivan
Eades, Susan
Ing, Nancy
Chapkin, Robert S.
author_facet Coleman, Michelle C.
Whitfield-Cargile, Canaan
Cohen, Noah D.
Goldsby, Jennifer L.
Davidson, Laurie
Chamoun-Emanuelli, Ana M.
Ivanov, Ivan
Eades, Susan
Ing, Nancy
Chapkin, Robert S.
author_sort Coleman, Michelle C.
collection PubMed
description Evaluating the health and function of the gastrointestinal tract can be challenging in all species, but is especially difficult in horses due to their size and length of the gastrointestinal (GI) tract. Isolation of mRNA of cells exfoliated from the GI mucosa into feces (i.e., the exfoliome) offers a novel means of non-invasively examining the gene expression profile of the GI mucosa. This approach has been utilized in people with colorectal cancer. Moreover, we have utilized this approach in a murine model of GI inflammation and demonstrated that the exfoliome reflects the tissue transcriptome. The ability of the equine exfoliome to provide non-invasive information regarding the health and function of the GI tract is not known. The objective of this study was to characterize the gene expression profile found in exfoliated intestinal epithelial cells from normal horses and compare the exfoliome data with the tissue mucosal transcriptome. Mucosal samples were collected from standardized locations along the GI tract (i.e. ileum, cecum, right dorsal colon, and rectum) from four healthy horses immediately following euthanasia. Voided feces were also collected. RNA isolation, library preparation, and RNA sequencing was performed on fecal and intestinal mucosal samples. Comparison of gene expression profiles from the tissue and exfoliome revealed correlation of gene expression. Moreover, the exfoliome contained reads representing the diverse array of cell types found in the GI mucosa suggesting the equine exfoliome serves as a non-invasive means of examining the global gene expression pattern of the equine GI tract.
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spelling pubmed-70755542020-03-23 Non-invasive evaluation of the equine gastrointestinal mucosal transcriptome Coleman, Michelle C. Whitfield-Cargile, Canaan Cohen, Noah D. Goldsby, Jennifer L. Davidson, Laurie Chamoun-Emanuelli, Ana M. Ivanov, Ivan Eades, Susan Ing, Nancy Chapkin, Robert S. PLoS One Research Article Evaluating the health and function of the gastrointestinal tract can be challenging in all species, but is especially difficult in horses due to their size and length of the gastrointestinal (GI) tract. Isolation of mRNA of cells exfoliated from the GI mucosa into feces (i.e., the exfoliome) offers a novel means of non-invasively examining the gene expression profile of the GI mucosa. This approach has been utilized in people with colorectal cancer. Moreover, we have utilized this approach in a murine model of GI inflammation and demonstrated that the exfoliome reflects the tissue transcriptome. The ability of the equine exfoliome to provide non-invasive information regarding the health and function of the GI tract is not known. The objective of this study was to characterize the gene expression profile found in exfoliated intestinal epithelial cells from normal horses and compare the exfoliome data with the tissue mucosal transcriptome. Mucosal samples were collected from standardized locations along the GI tract (i.e. ileum, cecum, right dorsal colon, and rectum) from four healthy horses immediately following euthanasia. Voided feces were also collected. RNA isolation, library preparation, and RNA sequencing was performed on fecal and intestinal mucosal samples. Comparison of gene expression profiles from the tissue and exfoliome revealed correlation of gene expression. Moreover, the exfoliome contained reads representing the diverse array of cell types found in the GI mucosa suggesting the equine exfoliome serves as a non-invasive means of examining the global gene expression pattern of the equine GI tract. Public Library of Science 2020-03-16 /pmc/articles/PMC7075554/ /pubmed/32176710 http://dx.doi.org/10.1371/journal.pone.0229797 Text en © 2020 Coleman et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Coleman, Michelle C.
Whitfield-Cargile, Canaan
Cohen, Noah D.
Goldsby, Jennifer L.
Davidson, Laurie
Chamoun-Emanuelli, Ana M.
Ivanov, Ivan
Eades, Susan
Ing, Nancy
Chapkin, Robert S.
Non-invasive evaluation of the equine gastrointestinal mucosal transcriptome
title Non-invasive evaluation of the equine gastrointestinal mucosal transcriptome
title_full Non-invasive evaluation of the equine gastrointestinal mucosal transcriptome
title_fullStr Non-invasive evaluation of the equine gastrointestinal mucosal transcriptome
title_full_unstemmed Non-invasive evaluation of the equine gastrointestinal mucosal transcriptome
title_short Non-invasive evaluation of the equine gastrointestinal mucosal transcriptome
title_sort non-invasive evaluation of the equine gastrointestinal mucosal transcriptome
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7075554/
https://www.ncbi.nlm.nih.gov/pubmed/32176710
http://dx.doi.org/10.1371/journal.pone.0229797
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