Cargando…
Expression of Cellular Receptors in the Ischemic Hemisphere of Mice with Increased Glucose Uptake
Many previous studies have shown reduced glucose uptake in the ischemic brain. In contrast, in a permanent unilateral common carotid artery occlusion (UCCAO) mouse model, our pilot experiments using 18F-fluorodeoxyglucose positron emission tomography (FDG PET) revealed that a subset of mice exhibite...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Korean Society for Brain and Neural Sciences
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7075656/ https://www.ncbi.nlm.nih.gov/pubmed/32122109 http://dx.doi.org/10.5607/en.2020.29.1.70 |
_version_ | 1783507067831582720 |
---|---|
author | Lee, Jin Soo Hong, Ji Man Yoon, Bok Seon Son, Keoung Sun Lee, Kyung Eon Im, Doo Soon Park, Bok-Nam An, Young-Sil Hwang, Dong Hoon Park, Chan Bae Kim, Byung Gon Joe, Eun-hye |
author_facet | Lee, Jin Soo Hong, Ji Man Yoon, Bok Seon Son, Keoung Sun Lee, Kyung Eon Im, Doo Soon Park, Bok-Nam An, Young-Sil Hwang, Dong Hoon Park, Chan Bae Kim, Byung Gon Joe, Eun-hye |
author_sort | Lee, Jin Soo |
collection | PubMed |
description | Many previous studies have shown reduced glucose uptake in the ischemic brain. In contrast, in a permanent unilateral common carotid artery occlusion (UCCAO) mouse model, our pilot experiments using 18F-fluorodeoxyglucose positron emission tomography (FDG PET) revealed that a subset of mice exhibited conspicuously high uptake of glucose in the ipsilateral hemisphere at 1 week post-occlusion (asymmetric group), whereas other mice showed symmetric uptake in both hemispheres (symmetric group). Thus, we aimed to understand the discrepancy between the two groups. Cerebral blood flow and histological/metabolic changes were analyzed using laser Doppler flowmetry and immunohistochemistry/Western blotting, respectively. Contrary to the increased glucose uptake observed in the ischemic cerebral hemisphere on FDG PET (p<0.001), cerebral blood flow tended to be lower in the asymmetric group than in the symmetric group (right to left ratio [%], 36.4±21.8 vs. 58.0±24.8, p=0.059). Neuronal death was observed only in the ischemic hemisphere of the asymmetric group. In contrast, astrocytes were more activated in the asymmetric group than in the symmetric group (p<0.05). Glucose transporter-1, and monocarboxylate transporter-1 were also upregulated in the asymmetric group, compared with the symmetric group (p<0.05, respectively). These results suggest that the increased FDG uptake was associated with relatively severe ischemia, and glucose transporter-1 upregulation and astrocyte activation. Glucose metabolism may thus be a compensatory mechanism in the moderately severe ischemic brain. |
format | Online Article Text |
id | pubmed-7075656 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | The Korean Society for Brain and Neural Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-70756562020-03-23 Expression of Cellular Receptors in the Ischemic Hemisphere of Mice with Increased Glucose Uptake Lee, Jin Soo Hong, Ji Man Yoon, Bok Seon Son, Keoung Sun Lee, Kyung Eon Im, Doo Soon Park, Bok-Nam An, Young-Sil Hwang, Dong Hoon Park, Chan Bae Kim, Byung Gon Joe, Eun-hye Exp Neurobiol Original Article Many previous studies have shown reduced glucose uptake in the ischemic brain. In contrast, in a permanent unilateral common carotid artery occlusion (UCCAO) mouse model, our pilot experiments using 18F-fluorodeoxyglucose positron emission tomography (FDG PET) revealed that a subset of mice exhibited conspicuously high uptake of glucose in the ipsilateral hemisphere at 1 week post-occlusion (asymmetric group), whereas other mice showed symmetric uptake in both hemispheres (symmetric group). Thus, we aimed to understand the discrepancy between the two groups. Cerebral blood flow and histological/metabolic changes were analyzed using laser Doppler flowmetry and immunohistochemistry/Western blotting, respectively. Contrary to the increased glucose uptake observed in the ischemic cerebral hemisphere on FDG PET (p<0.001), cerebral blood flow tended to be lower in the asymmetric group than in the symmetric group (right to left ratio [%], 36.4±21.8 vs. 58.0±24.8, p=0.059). Neuronal death was observed only in the ischemic hemisphere of the asymmetric group. In contrast, astrocytes were more activated in the asymmetric group than in the symmetric group (p<0.05). Glucose transporter-1, and monocarboxylate transporter-1 were also upregulated in the asymmetric group, compared with the symmetric group (p<0.05, respectively). These results suggest that the increased FDG uptake was associated with relatively severe ischemia, and glucose transporter-1 upregulation and astrocyte activation. Glucose metabolism may thus be a compensatory mechanism in the moderately severe ischemic brain. The Korean Society for Brain and Neural Sciences 2020-02 2020-02-29 /pmc/articles/PMC7075656/ /pubmed/32122109 http://dx.doi.org/10.5607/en.2020.29.1.70 Text en Copyright © Experimental Neurobiology 2020 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Lee, Jin Soo Hong, Ji Man Yoon, Bok Seon Son, Keoung Sun Lee, Kyung Eon Im, Doo Soon Park, Bok-Nam An, Young-Sil Hwang, Dong Hoon Park, Chan Bae Kim, Byung Gon Joe, Eun-hye Expression of Cellular Receptors in the Ischemic Hemisphere of Mice with Increased Glucose Uptake |
title | Expression of Cellular Receptors in the Ischemic Hemisphere of Mice with Increased Glucose Uptake |
title_full | Expression of Cellular Receptors in the Ischemic Hemisphere of Mice with Increased Glucose Uptake |
title_fullStr | Expression of Cellular Receptors in the Ischemic Hemisphere of Mice with Increased Glucose Uptake |
title_full_unstemmed | Expression of Cellular Receptors in the Ischemic Hemisphere of Mice with Increased Glucose Uptake |
title_short | Expression of Cellular Receptors in the Ischemic Hemisphere of Mice with Increased Glucose Uptake |
title_sort | expression of cellular receptors in the ischemic hemisphere of mice with increased glucose uptake |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7075656/ https://www.ncbi.nlm.nih.gov/pubmed/32122109 http://dx.doi.org/10.5607/en.2020.29.1.70 |
work_keys_str_mv | AT leejinsoo expressionofcellularreceptorsintheischemichemisphereofmicewithincreasedglucoseuptake AT hongjiman expressionofcellularreceptorsintheischemichemisphereofmicewithincreasedglucoseuptake AT yoonbokseon expressionofcellularreceptorsintheischemichemisphereofmicewithincreasedglucoseuptake AT sonkeoungsun expressionofcellularreceptorsintheischemichemisphereofmicewithincreasedglucoseuptake AT leekyungeon expressionofcellularreceptorsintheischemichemisphereofmicewithincreasedglucoseuptake AT imdoosoon expressionofcellularreceptorsintheischemichemisphereofmicewithincreasedglucoseuptake AT parkboknam expressionofcellularreceptorsintheischemichemisphereofmicewithincreasedglucoseuptake AT anyoungsil expressionofcellularreceptorsintheischemichemisphereofmicewithincreasedglucoseuptake AT hwangdonghoon expressionofcellularreceptorsintheischemichemisphereofmicewithincreasedglucoseuptake AT parkchanbae expressionofcellularreceptorsintheischemichemisphereofmicewithincreasedglucoseuptake AT kimbyunggon expressionofcellularreceptorsintheischemichemisphereofmicewithincreasedglucoseuptake AT joeeunhye expressionofcellularreceptorsintheischemichemisphereofmicewithincreasedglucoseuptake |