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Expression of Cellular Receptors in the Ischemic Hemisphere of Mice with Increased Glucose Uptake

Many previous studies have shown reduced glucose uptake in the ischemic brain. In contrast, in a permanent unilateral common carotid artery occlusion (UCCAO) mouse model, our pilot experiments using 18F-fluorodeoxyglucose positron emission tomography (FDG PET) revealed that a subset of mice exhibite...

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Autores principales: Lee, Jin Soo, Hong, Ji Man, Yoon, Bok Seon, Son, Keoung Sun, Lee, Kyung Eon, Im, Doo Soon, Park, Bok-Nam, An, Young-Sil, Hwang, Dong Hoon, Park, Chan Bae, Kim, Byung Gon, Joe, Eun-hye
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society for Brain and Neural Sciences 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7075656/
https://www.ncbi.nlm.nih.gov/pubmed/32122109
http://dx.doi.org/10.5607/en.2020.29.1.70
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author Lee, Jin Soo
Hong, Ji Man
Yoon, Bok Seon
Son, Keoung Sun
Lee, Kyung Eon
Im, Doo Soon
Park, Bok-Nam
An, Young-Sil
Hwang, Dong Hoon
Park, Chan Bae
Kim, Byung Gon
Joe, Eun-hye
author_facet Lee, Jin Soo
Hong, Ji Man
Yoon, Bok Seon
Son, Keoung Sun
Lee, Kyung Eon
Im, Doo Soon
Park, Bok-Nam
An, Young-Sil
Hwang, Dong Hoon
Park, Chan Bae
Kim, Byung Gon
Joe, Eun-hye
author_sort Lee, Jin Soo
collection PubMed
description Many previous studies have shown reduced glucose uptake in the ischemic brain. In contrast, in a permanent unilateral common carotid artery occlusion (UCCAO) mouse model, our pilot experiments using 18F-fluorodeoxyglucose positron emission tomography (FDG PET) revealed that a subset of mice exhibited conspicuously high uptake of glucose in the ipsilateral hemisphere at 1 week post-occlusion (asymmetric group), whereas other mice showed symmetric uptake in both hemispheres (symmetric group). Thus, we aimed to understand the discrepancy between the two groups. Cerebral blood flow and histological/metabolic changes were analyzed using laser Doppler flowmetry and immunohistochemistry/Western blotting, respectively. Contrary to the increased glucose uptake observed in the ischemic cerebral hemisphere on FDG PET (p<0.001), cerebral blood flow tended to be lower in the asymmetric group than in the symmetric group (right to left ratio [%], 36.4±21.8 vs. 58.0±24.8, p=0.059). Neuronal death was observed only in the ischemic hemisphere of the asymmetric group. In contrast, astrocytes were more activated in the asymmetric group than in the symmetric group (p<0.05). Glucose transporter-1, and monocarboxylate transporter-1 were also upregulated in the asymmetric group, compared with the symmetric group (p<0.05, respectively). These results suggest that the increased FDG uptake was associated with relatively severe ischemia, and glucose transporter-1 upregulation and astrocyte activation. Glucose metabolism may thus be a compensatory mechanism in the moderately severe ischemic brain.
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spelling pubmed-70756562020-03-23 Expression of Cellular Receptors in the Ischemic Hemisphere of Mice with Increased Glucose Uptake Lee, Jin Soo Hong, Ji Man Yoon, Bok Seon Son, Keoung Sun Lee, Kyung Eon Im, Doo Soon Park, Bok-Nam An, Young-Sil Hwang, Dong Hoon Park, Chan Bae Kim, Byung Gon Joe, Eun-hye Exp Neurobiol Original Article Many previous studies have shown reduced glucose uptake in the ischemic brain. In contrast, in a permanent unilateral common carotid artery occlusion (UCCAO) mouse model, our pilot experiments using 18F-fluorodeoxyglucose positron emission tomography (FDG PET) revealed that a subset of mice exhibited conspicuously high uptake of glucose in the ipsilateral hemisphere at 1 week post-occlusion (asymmetric group), whereas other mice showed symmetric uptake in both hemispheres (symmetric group). Thus, we aimed to understand the discrepancy between the two groups. Cerebral blood flow and histological/metabolic changes were analyzed using laser Doppler flowmetry and immunohistochemistry/Western blotting, respectively. Contrary to the increased glucose uptake observed in the ischemic cerebral hemisphere on FDG PET (p<0.001), cerebral blood flow tended to be lower in the asymmetric group than in the symmetric group (right to left ratio [%], 36.4±21.8 vs. 58.0±24.8, p=0.059). Neuronal death was observed only in the ischemic hemisphere of the asymmetric group. In contrast, astrocytes were more activated in the asymmetric group than in the symmetric group (p<0.05). Glucose transporter-1, and monocarboxylate transporter-1 were also upregulated in the asymmetric group, compared with the symmetric group (p<0.05, respectively). These results suggest that the increased FDG uptake was associated with relatively severe ischemia, and glucose transporter-1 upregulation and astrocyte activation. Glucose metabolism may thus be a compensatory mechanism in the moderately severe ischemic brain. The Korean Society for Brain and Neural Sciences 2020-02 2020-02-29 /pmc/articles/PMC7075656/ /pubmed/32122109 http://dx.doi.org/10.5607/en.2020.29.1.70 Text en Copyright © Experimental Neurobiology 2020 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Lee, Jin Soo
Hong, Ji Man
Yoon, Bok Seon
Son, Keoung Sun
Lee, Kyung Eon
Im, Doo Soon
Park, Bok-Nam
An, Young-Sil
Hwang, Dong Hoon
Park, Chan Bae
Kim, Byung Gon
Joe, Eun-hye
Expression of Cellular Receptors in the Ischemic Hemisphere of Mice with Increased Glucose Uptake
title Expression of Cellular Receptors in the Ischemic Hemisphere of Mice with Increased Glucose Uptake
title_full Expression of Cellular Receptors in the Ischemic Hemisphere of Mice with Increased Glucose Uptake
title_fullStr Expression of Cellular Receptors in the Ischemic Hemisphere of Mice with Increased Glucose Uptake
title_full_unstemmed Expression of Cellular Receptors in the Ischemic Hemisphere of Mice with Increased Glucose Uptake
title_short Expression of Cellular Receptors in the Ischemic Hemisphere of Mice with Increased Glucose Uptake
title_sort expression of cellular receptors in the ischemic hemisphere of mice with increased glucose uptake
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7075656/
https://www.ncbi.nlm.nih.gov/pubmed/32122109
http://dx.doi.org/10.5607/en.2020.29.1.70
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