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In vivo dynamic analysis of BMP-2-induced ectopic bone formation

Bone morphogenetic protein (BMP)-2 plays a central role in bone-tissue engineering because of its potent bone-induction ability. However, the process of BMP-induced bone formation in vivo remains poorly elucidated. Here, we aimed to establish a method for intravital imaging of the entire process of...

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Autores principales: Hashimoto, Kunihiko, Kaito, Takashi, Furuya, Masayuki, Seno, Shigeto, Okuzaki, Daisuke, Kikuta, Junichi, Tsukazaki, Hiroyuki, Matsuda, Hideo, Yoshikawa, Hideki, Ishii, Masaru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7076033/
https://www.ncbi.nlm.nih.gov/pubmed/32179857
http://dx.doi.org/10.1038/s41598-020-61825-2
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author Hashimoto, Kunihiko
Kaito, Takashi
Furuya, Masayuki
Seno, Shigeto
Okuzaki, Daisuke
Kikuta, Junichi
Tsukazaki, Hiroyuki
Matsuda, Hideo
Yoshikawa, Hideki
Ishii, Masaru
author_facet Hashimoto, Kunihiko
Kaito, Takashi
Furuya, Masayuki
Seno, Shigeto
Okuzaki, Daisuke
Kikuta, Junichi
Tsukazaki, Hiroyuki
Matsuda, Hideo
Yoshikawa, Hideki
Ishii, Masaru
author_sort Hashimoto, Kunihiko
collection PubMed
description Bone morphogenetic protein (BMP)-2 plays a central role in bone-tissue engineering because of its potent bone-induction ability. However, the process of BMP-induced bone formation in vivo remains poorly elucidated. Here, we aimed to establish a method for intravital imaging of the entire process of BMP-2-induced ectopic bone formation. Using multicolor intravital imaging in transgenic mice, we visualized the spatiotemporal process of bone induction, including appearance and motility of osteoblasts and osteoclasts, angiogenesis, collagen-fiber formation, and bone-mineral deposition. Furthermore, we investigated how PTH1-34 affects BMP-2-induced bone formation, which revealed that PTH1-34 administration accelerated differentiation and increased the motility of osteoblasts, whereas it decreased morphological changes in osteoclasts. This is the first report on visualization of the entire process of BMP-2-induced bone formation using intravital imaging techniques, which, we believe, will contribute to our understanding of ectopic bone formation and provide new parameters for evaluating bone-forming activity.
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spelling pubmed-70760332020-03-23 In vivo dynamic analysis of BMP-2-induced ectopic bone formation Hashimoto, Kunihiko Kaito, Takashi Furuya, Masayuki Seno, Shigeto Okuzaki, Daisuke Kikuta, Junichi Tsukazaki, Hiroyuki Matsuda, Hideo Yoshikawa, Hideki Ishii, Masaru Sci Rep Article Bone morphogenetic protein (BMP)-2 plays a central role in bone-tissue engineering because of its potent bone-induction ability. However, the process of BMP-induced bone formation in vivo remains poorly elucidated. Here, we aimed to establish a method for intravital imaging of the entire process of BMP-2-induced ectopic bone formation. Using multicolor intravital imaging in transgenic mice, we visualized the spatiotemporal process of bone induction, including appearance and motility of osteoblasts and osteoclasts, angiogenesis, collagen-fiber formation, and bone-mineral deposition. Furthermore, we investigated how PTH1-34 affects BMP-2-induced bone formation, which revealed that PTH1-34 administration accelerated differentiation and increased the motility of osteoblasts, whereas it decreased morphological changes in osteoclasts. This is the first report on visualization of the entire process of BMP-2-induced bone formation using intravital imaging techniques, which, we believe, will contribute to our understanding of ectopic bone formation and provide new parameters for evaluating bone-forming activity. Nature Publishing Group UK 2020-03-16 /pmc/articles/PMC7076033/ /pubmed/32179857 http://dx.doi.org/10.1038/s41598-020-61825-2 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Hashimoto, Kunihiko
Kaito, Takashi
Furuya, Masayuki
Seno, Shigeto
Okuzaki, Daisuke
Kikuta, Junichi
Tsukazaki, Hiroyuki
Matsuda, Hideo
Yoshikawa, Hideki
Ishii, Masaru
In vivo dynamic analysis of BMP-2-induced ectopic bone formation
title In vivo dynamic analysis of BMP-2-induced ectopic bone formation
title_full In vivo dynamic analysis of BMP-2-induced ectopic bone formation
title_fullStr In vivo dynamic analysis of BMP-2-induced ectopic bone formation
title_full_unstemmed In vivo dynamic analysis of BMP-2-induced ectopic bone formation
title_short In vivo dynamic analysis of BMP-2-induced ectopic bone formation
title_sort in vivo dynamic analysis of bmp-2-induced ectopic bone formation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7076033/
https://www.ncbi.nlm.nih.gov/pubmed/32179857
http://dx.doi.org/10.1038/s41598-020-61825-2
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