Cargando…

Stimulation of Insect Herbivory by Elevated Temperature Outweighs Protection by the Jasmonate Pathway

Rising global temperatures are associated with increases in the geographic range, population size, and feeding voracity of insect herbivores. Although it is well established that the plant hormone jasmonate (JA) promotes durable resistance to many ectothermic herbivores, little is known about how JA...

Descripción completa

Detalles Bibliográficos
Autores principales: Havko, Nathan E., Kapali, George, Das, Michael R., Howe, Gregg A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7076421/
https://www.ncbi.nlm.nih.gov/pubmed/32024094
http://dx.doi.org/10.3390/plants9020172
_version_ 1783507212827623424
author Havko, Nathan E.
Kapali, George
Das, Michael R.
Howe, Gregg A.
author_facet Havko, Nathan E.
Kapali, George
Das, Michael R.
Howe, Gregg A.
author_sort Havko, Nathan E.
collection PubMed
description Rising global temperatures are associated with increases in the geographic range, population size, and feeding voracity of insect herbivores. Although it is well established that the plant hormone jasmonate (JA) promotes durable resistance to many ectothermic herbivores, little is known about how JA-mediated defense is influenced by rising temperatures. Here, we used the Arabidopsis-Trichoplusia ni (cabbage looper) interaction to investigate the relative contribution of JA and elevated temperature to host resistance. Video monitoring of T. ni larval behavior showed that elevated temperature greatly enhanced defoliation by increasing the bite rate and total time spent feeding, whereas loss of resistance in a JA-deficient mutant did not strongly affect these behaviors. The acceleration of insect feeding at elevated temperature was not attributed to decreases in wound-induced JA biosynthesis, expression of JA-responsive genes, or the accumulation of defensive glucosinolates prior to insect challenge. Quantitative proteomic analysis of insect frass, however, provided evidence for a temperature-dependent increase in the production of T. ni digestive enzymes. Our results demonstrate that temperature-driven stimulation of T. ni feeding outweighs the protective effects of JA-mediated resistance in Arabidopsis, thus highlighting a potential threat to plant resilience in a warming world.
format Online
Article
Text
id pubmed-7076421
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-70764212020-03-24 Stimulation of Insect Herbivory by Elevated Temperature Outweighs Protection by the Jasmonate Pathway Havko, Nathan E. Kapali, George Das, Michael R. Howe, Gregg A. Plants (Basel) Article Rising global temperatures are associated with increases in the geographic range, population size, and feeding voracity of insect herbivores. Although it is well established that the plant hormone jasmonate (JA) promotes durable resistance to many ectothermic herbivores, little is known about how JA-mediated defense is influenced by rising temperatures. Here, we used the Arabidopsis-Trichoplusia ni (cabbage looper) interaction to investigate the relative contribution of JA and elevated temperature to host resistance. Video monitoring of T. ni larval behavior showed that elevated temperature greatly enhanced defoliation by increasing the bite rate and total time spent feeding, whereas loss of resistance in a JA-deficient mutant did not strongly affect these behaviors. The acceleration of insect feeding at elevated temperature was not attributed to decreases in wound-induced JA biosynthesis, expression of JA-responsive genes, or the accumulation of defensive glucosinolates prior to insect challenge. Quantitative proteomic analysis of insect frass, however, provided evidence for a temperature-dependent increase in the production of T. ni digestive enzymes. Our results demonstrate that temperature-driven stimulation of T. ni feeding outweighs the protective effects of JA-mediated resistance in Arabidopsis, thus highlighting a potential threat to plant resilience in a warming world. MDPI 2020-02-01 /pmc/articles/PMC7076421/ /pubmed/32024094 http://dx.doi.org/10.3390/plants9020172 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Havko, Nathan E.
Kapali, George
Das, Michael R.
Howe, Gregg A.
Stimulation of Insect Herbivory by Elevated Temperature Outweighs Protection by the Jasmonate Pathway
title Stimulation of Insect Herbivory by Elevated Temperature Outweighs Protection by the Jasmonate Pathway
title_full Stimulation of Insect Herbivory by Elevated Temperature Outweighs Protection by the Jasmonate Pathway
title_fullStr Stimulation of Insect Herbivory by Elevated Temperature Outweighs Protection by the Jasmonate Pathway
title_full_unstemmed Stimulation of Insect Herbivory by Elevated Temperature Outweighs Protection by the Jasmonate Pathway
title_short Stimulation of Insect Herbivory by Elevated Temperature Outweighs Protection by the Jasmonate Pathway
title_sort stimulation of insect herbivory by elevated temperature outweighs protection by the jasmonate pathway
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7076421/
https://www.ncbi.nlm.nih.gov/pubmed/32024094
http://dx.doi.org/10.3390/plants9020172
work_keys_str_mv AT havkonathane stimulationofinsectherbivorybyelevatedtemperatureoutweighsprotectionbythejasmonatepathway
AT kapaligeorge stimulationofinsectherbivorybyelevatedtemperatureoutweighsprotectionbythejasmonatepathway
AT dasmichaelr stimulationofinsectherbivorybyelevatedtemperatureoutweighsprotectionbythejasmonatepathway
AT howegregga stimulationofinsectherbivorybyelevatedtemperatureoutweighsprotectionbythejasmonatepathway