Cargando…
17β-Estradiol Delivered in Eye Drops: Evidence of Impact on Protein Networks and Associated Biological Processes in the Rat Retina through Quantitative Proteomics
To facilitate the development of broad-spectrum retina neuroprotectants that can be delivered through topical dosage forms, this proteomics study focused on analyzing target engagements through the identification of functional protein networks impacted after delivery of 17β-estradiol in eye drops. S...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7076522/ https://www.ncbi.nlm.nih.gov/pubmed/32012756 http://dx.doi.org/10.3390/pharmaceutics12020101 |
_version_ | 1783507236302094336 |
---|---|
author | Prokai, Laszlo Zaman, Khadiza Nguyen, Vien Prokai-Tatrai, Katalin |
author_facet | Prokai, Laszlo Zaman, Khadiza Nguyen, Vien Prokai-Tatrai, Katalin |
author_sort | Prokai, Laszlo |
collection | PubMed |
description | To facilitate the development of broad-spectrum retina neuroprotectants that can be delivered through topical dosage forms, this proteomics study focused on analyzing target engagements through the identification of functional protein networks impacted after delivery of 17β-estradiol in eye drops. Specifically, the retinae of ovariectomized Brown Norway rats treated with daily eye drops of 17β-estradiol for three weeks were compared to those of vehicle-treated ovariectomized control animals. We searched the acquired raw data against a composite protein sequence database by using Mascot, as well as employed label-free quantification to detect changes in protein abundances. Our investigation using rigorous validation criteria revealed 331 estrogen-regulated proteins in the rat retina (158 were up-regulated, while 173 were down-regulated by 17β-estradiol delivered in eye drops). Comprehensive pathway analyses indicate that these proteins are relevant overall to nervous system development and function, tissue development, organ development, as well as visual system development and function. We also present 18 protein networks with associated canonical pathways showing the effects of treatments for the detailed analyses of target engagements regarding potential application of estrogens as topically delivered broad-spectrum retina neuroprotectants. Profound impact on crystallins is discussed as one of the plausible neuroprotective mechanisms. |
format | Online Article Text |
id | pubmed-7076522 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-70765222020-03-20 17β-Estradiol Delivered in Eye Drops: Evidence of Impact on Protein Networks and Associated Biological Processes in the Rat Retina through Quantitative Proteomics Prokai, Laszlo Zaman, Khadiza Nguyen, Vien Prokai-Tatrai, Katalin Pharmaceutics Article To facilitate the development of broad-spectrum retina neuroprotectants that can be delivered through topical dosage forms, this proteomics study focused on analyzing target engagements through the identification of functional protein networks impacted after delivery of 17β-estradiol in eye drops. Specifically, the retinae of ovariectomized Brown Norway rats treated with daily eye drops of 17β-estradiol for three weeks were compared to those of vehicle-treated ovariectomized control animals. We searched the acquired raw data against a composite protein sequence database by using Mascot, as well as employed label-free quantification to detect changes in protein abundances. Our investigation using rigorous validation criteria revealed 331 estrogen-regulated proteins in the rat retina (158 were up-regulated, while 173 were down-regulated by 17β-estradiol delivered in eye drops). Comprehensive pathway analyses indicate that these proteins are relevant overall to nervous system development and function, tissue development, organ development, as well as visual system development and function. We also present 18 protein networks with associated canonical pathways showing the effects of treatments for the detailed analyses of target engagements regarding potential application of estrogens as topically delivered broad-spectrum retina neuroprotectants. Profound impact on crystallins is discussed as one of the plausible neuroprotective mechanisms. MDPI 2020-01-27 /pmc/articles/PMC7076522/ /pubmed/32012756 http://dx.doi.org/10.3390/pharmaceutics12020101 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Prokai, Laszlo Zaman, Khadiza Nguyen, Vien Prokai-Tatrai, Katalin 17β-Estradiol Delivered in Eye Drops: Evidence of Impact on Protein Networks and Associated Biological Processes in the Rat Retina through Quantitative Proteomics |
title | 17β-Estradiol Delivered in Eye Drops: Evidence of Impact on Protein Networks and Associated Biological Processes in the Rat Retina through Quantitative Proteomics |
title_full | 17β-Estradiol Delivered in Eye Drops: Evidence of Impact on Protein Networks and Associated Biological Processes in the Rat Retina through Quantitative Proteomics |
title_fullStr | 17β-Estradiol Delivered in Eye Drops: Evidence of Impact on Protein Networks and Associated Biological Processes in the Rat Retina through Quantitative Proteomics |
title_full_unstemmed | 17β-Estradiol Delivered in Eye Drops: Evidence of Impact on Protein Networks and Associated Biological Processes in the Rat Retina through Quantitative Proteomics |
title_short | 17β-Estradiol Delivered in Eye Drops: Evidence of Impact on Protein Networks and Associated Biological Processes in the Rat Retina through Quantitative Proteomics |
title_sort | 17β-estradiol delivered in eye drops: evidence of impact on protein networks and associated biological processes in the rat retina through quantitative proteomics |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7076522/ https://www.ncbi.nlm.nih.gov/pubmed/32012756 http://dx.doi.org/10.3390/pharmaceutics12020101 |
work_keys_str_mv | AT prokailaszlo 17bestradioldeliveredineyedropsevidenceofimpactonproteinnetworksandassociatedbiologicalprocessesintheratretinathroughquantitativeproteomics AT zamankhadiza 17bestradioldeliveredineyedropsevidenceofimpactonproteinnetworksandassociatedbiologicalprocessesintheratretinathroughquantitativeproteomics AT nguyenvien 17bestradioldeliveredineyedropsevidenceofimpactonproteinnetworksandassociatedbiologicalprocessesintheratretinathroughquantitativeproteomics AT prokaitatraikatalin 17bestradioldeliveredineyedropsevidenceofimpactonproteinnetworksandassociatedbiologicalprocessesintheratretinathroughquantitativeproteomics |