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Long-Chain acyl-CoA Synthetase LACS2 Contributes to Submergence Tolerance by Modulating Cuticle Permeability in Arabidopsis

In Arabidopsis thaliana, LONG-CHAIN ACYL-COA SYNTHETASEs (LACSs) catalyze the synthesis of long-chain acyl-CoAs and function in diverse biological processes. We have recently revealed that LACS2 is primarily involved in the production of polyunsaturated linolenoyl-CoA, essential for the activation o...

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Autores principales: Xie, Li-Juan, Tan, Wei-Juan, Yang, Yi-Cong, Tan, Yi-Fang, Zhou, Ying, Zhou, De-Mian, Xiao, Shi, Chen, Qin-Fang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7076686/
https://www.ncbi.nlm.nih.gov/pubmed/32085442
http://dx.doi.org/10.3390/plants9020262
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author Xie, Li-Juan
Tan, Wei-Juan
Yang, Yi-Cong
Tan, Yi-Fang
Zhou, Ying
Zhou, De-Mian
Xiao, Shi
Chen, Qin-Fang
author_facet Xie, Li-Juan
Tan, Wei-Juan
Yang, Yi-Cong
Tan, Yi-Fang
Zhou, Ying
Zhou, De-Mian
Xiao, Shi
Chen, Qin-Fang
author_sort Xie, Li-Juan
collection PubMed
description In Arabidopsis thaliana, LONG-CHAIN ACYL-COA SYNTHETASEs (LACSs) catalyze the synthesis of long-chain acyl-CoAs and function in diverse biological processes. We have recently revealed that LACS2 is primarily involved in the production of polyunsaturated linolenoyl-CoA, essential for the activation of ethylene response transcription factors-mediated hypoxia signaling. Here, we further reported the dual role of LACS2 in the regulation of submergence tolerance by modulating cuticle permeability in Arabidopsis cells. LACS2-overexpressors (LACS2-OEs) showed improved tolerance to submergence, with higher accumulation of cuticular wax and cutin in their rosettes. In contrast, knockout of LACS2 in the lacs2-3 mutant resulted in hypersensitivity to submergence with reduced wax crystals and thinner cutin layer. By analyses of plant surface permeability, we observed that the hypoxic sensitivities in the LACS2-OEs and lacs2-3 mutant were physiologically correlated with chlorophyll leaching, water loss rates, ionic leakage, and gas exchange. Thus, our findings suggest the role of LACS2 in plant response to submergence by modulating cuticle permeability in plant cells.
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spelling pubmed-70766862020-03-20 Long-Chain acyl-CoA Synthetase LACS2 Contributes to Submergence Tolerance by Modulating Cuticle Permeability in Arabidopsis Xie, Li-Juan Tan, Wei-Juan Yang, Yi-Cong Tan, Yi-Fang Zhou, Ying Zhou, De-Mian Xiao, Shi Chen, Qin-Fang Plants (Basel) Article In Arabidopsis thaliana, LONG-CHAIN ACYL-COA SYNTHETASEs (LACSs) catalyze the synthesis of long-chain acyl-CoAs and function in diverse biological processes. We have recently revealed that LACS2 is primarily involved in the production of polyunsaturated linolenoyl-CoA, essential for the activation of ethylene response transcription factors-mediated hypoxia signaling. Here, we further reported the dual role of LACS2 in the regulation of submergence tolerance by modulating cuticle permeability in Arabidopsis cells. LACS2-overexpressors (LACS2-OEs) showed improved tolerance to submergence, with higher accumulation of cuticular wax and cutin in their rosettes. In contrast, knockout of LACS2 in the lacs2-3 mutant resulted in hypersensitivity to submergence with reduced wax crystals and thinner cutin layer. By analyses of plant surface permeability, we observed that the hypoxic sensitivities in the LACS2-OEs and lacs2-3 mutant were physiologically correlated with chlorophyll leaching, water loss rates, ionic leakage, and gas exchange. Thus, our findings suggest the role of LACS2 in plant response to submergence by modulating cuticle permeability in plant cells. MDPI 2020-02-18 /pmc/articles/PMC7076686/ /pubmed/32085442 http://dx.doi.org/10.3390/plants9020262 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Xie, Li-Juan
Tan, Wei-Juan
Yang, Yi-Cong
Tan, Yi-Fang
Zhou, Ying
Zhou, De-Mian
Xiao, Shi
Chen, Qin-Fang
Long-Chain acyl-CoA Synthetase LACS2 Contributes to Submergence Tolerance by Modulating Cuticle Permeability in Arabidopsis
title Long-Chain acyl-CoA Synthetase LACS2 Contributes to Submergence Tolerance by Modulating Cuticle Permeability in Arabidopsis
title_full Long-Chain acyl-CoA Synthetase LACS2 Contributes to Submergence Tolerance by Modulating Cuticle Permeability in Arabidopsis
title_fullStr Long-Chain acyl-CoA Synthetase LACS2 Contributes to Submergence Tolerance by Modulating Cuticle Permeability in Arabidopsis
title_full_unstemmed Long-Chain acyl-CoA Synthetase LACS2 Contributes to Submergence Tolerance by Modulating Cuticle Permeability in Arabidopsis
title_short Long-Chain acyl-CoA Synthetase LACS2 Contributes to Submergence Tolerance by Modulating Cuticle Permeability in Arabidopsis
title_sort long-chain acyl-coa synthetase lacs2 contributes to submergence tolerance by modulating cuticle permeability in arabidopsis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7076686/
https://www.ncbi.nlm.nih.gov/pubmed/32085442
http://dx.doi.org/10.3390/plants9020262
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