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Mitochondria and Lysosomes Participate in Vip3Aa-Induced Spodoptera frugiperda Sf9 Cell Apoptosis

Vip3Aa, a soluble protein produced by certain Bacillus thuringiensis strains, is capable of inducing apoptosis in Sf9 cells. However, the apoptosis mechanism triggered by Vip3Aa is unclear. In this study, we found that Vip3Aa induces mitochondrial dysfunction, as evidenced by signs of collapse of mi...

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Autores principales: Hou, Xiaoyue, Han, Lu, An, Baoju, Zhang, Yanli, Cao, Zhanglei, Zhan, Yunda, Cai, Xia, Yan, Bing, Cai, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7076775/
https://www.ncbi.nlm.nih.gov/pubmed/32069858
http://dx.doi.org/10.3390/toxins12020116
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author Hou, Xiaoyue
Han, Lu
An, Baoju
Zhang, Yanli
Cao, Zhanglei
Zhan, Yunda
Cai, Xia
Yan, Bing
Cai, Jun
author_facet Hou, Xiaoyue
Han, Lu
An, Baoju
Zhang, Yanli
Cao, Zhanglei
Zhan, Yunda
Cai, Xia
Yan, Bing
Cai, Jun
author_sort Hou, Xiaoyue
collection PubMed
description Vip3Aa, a soluble protein produced by certain Bacillus thuringiensis strains, is capable of inducing apoptosis in Sf9 cells. However, the apoptosis mechanism triggered by Vip3Aa is unclear. In this study, we found that Vip3Aa induces mitochondrial dysfunction, as evidenced by signs of collapse of mitochondrial membrane potential, accumulation of reactive oxygen species, release of cytochrome c, and caspase-9 and -3 activation. Meanwhile, our results indicated that Vip3Aa reduces the ability of lysosomes in Sf9 cells to retain acridine orange. Moreover, pretreatment with Z-Phe-Tyr-CHO (a cathepsin L inhibitor) or pepstatin (a cathepsin D inhibitor) increased Sf9 cell viability, reduced cytochrome c release, and decreased caspase-9 and -3 activity. In conclusion, our findings suggested that Vip3Aa promotes Sf9 cell apoptosis by mitochondrial dysfunction, and lysosomes also play a vital role in the action of Vip3Aa.
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spelling pubmed-70767752020-03-20 Mitochondria and Lysosomes Participate in Vip3Aa-Induced Spodoptera frugiperda Sf9 Cell Apoptosis Hou, Xiaoyue Han, Lu An, Baoju Zhang, Yanli Cao, Zhanglei Zhan, Yunda Cai, Xia Yan, Bing Cai, Jun Toxins (Basel) Article Vip3Aa, a soluble protein produced by certain Bacillus thuringiensis strains, is capable of inducing apoptosis in Sf9 cells. However, the apoptosis mechanism triggered by Vip3Aa is unclear. In this study, we found that Vip3Aa induces mitochondrial dysfunction, as evidenced by signs of collapse of mitochondrial membrane potential, accumulation of reactive oxygen species, release of cytochrome c, and caspase-9 and -3 activation. Meanwhile, our results indicated that Vip3Aa reduces the ability of lysosomes in Sf9 cells to retain acridine orange. Moreover, pretreatment with Z-Phe-Tyr-CHO (a cathepsin L inhibitor) or pepstatin (a cathepsin D inhibitor) increased Sf9 cell viability, reduced cytochrome c release, and decreased caspase-9 and -3 activity. In conclusion, our findings suggested that Vip3Aa promotes Sf9 cell apoptosis by mitochondrial dysfunction, and lysosomes also play a vital role in the action of Vip3Aa. MDPI 2020-02-13 /pmc/articles/PMC7076775/ /pubmed/32069858 http://dx.doi.org/10.3390/toxins12020116 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hou, Xiaoyue
Han, Lu
An, Baoju
Zhang, Yanli
Cao, Zhanglei
Zhan, Yunda
Cai, Xia
Yan, Bing
Cai, Jun
Mitochondria and Lysosomes Participate in Vip3Aa-Induced Spodoptera frugiperda Sf9 Cell Apoptosis
title Mitochondria and Lysosomes Participate in Vip3Aa-Induced Spodoptera frugiperda Sf9 Cell Apoptosis
title_full Mitochondria and Lysosomes Participate in Vip3Aa-Induced Spodoptera frugiperda Sf9 Cell Apoptosis
title_fullStr Mitochondria and Lysosomes Participate in Vip3Aa-Induced Spodoptera frugiperda Sf9 Cell Apoptosis
title_full_unstemmed Mitochondria and Lysosomes Participate in Vip3Aa-Induced Spodoptera frugiperda Sf9 Cell Apoptosis
title_short Mitochondria and Lysosomes Participate in Vip3Aa-Induced Spodoptera frugiperda Sf9 Cell Apoptosis
title_sort mitochondria and lysosomes participate in vip3aa-induced spodoptera frugiperda sf9 cell apoptosis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7076775/
https://www.ncbi.nlm.nih.gov/pubmed/32069858
http://dx.doi.org/10.3390/toxins12020116
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