The application of metagenomic next-generation sequencing in diagnosing Chlamydia psittaci pneumonia: a report of five cases

BACKGROUND: Chlamydia psittaci pneumonia is a zoonotic infectious disease caused by Chlamydia psittaci. Diagnostic tools, including culture, serologic test and PCR-based methods, are available but prone to false negative results. CASE PRESENTATION: This report included five cases of Chlamydia psitta...

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Autores principales: Gu, Lei, Liu, Wei, Ru, Meng, Lin, Jing, Yu, Guoqing, Ye, Jia, Zhu, Zheng-an, Liu, Yuebin, Chen, Jian, Lai, Guoxiang, Wen, Wen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Case Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7077129/
https://www.ncbi.nlm.nih.gov/pubmed/32178660
http://dx.doi.org/10.1186/s12890-020-1098-x
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author Gu, Lei
Liu, Wei
Ru, Meng
Lin, Jing
Yu, Guoqing
Ye, Jia
Zhu, Zheng-an
Liu, Yuebin
Chen, Jian
Lai, Guoxiang
Wen, Wen
author_facet Gu, Lei
Liu, Wei
Ru, Meng
Lin, Jing
Yu, Guoqing
Ye, Jia
Zhu, Zheng-an
Liu, Yuebin
Chen, Jian
Lai, Guoxiang
Wen, Wen
author_sort Gu, Lei
collection PubMed
description BACKGROUND: Chlamydia psittaci pneumonia is a zoonotic infectious disease caused by Chlamydia psittaci. Diagnostic tools, including culture, serologic test and PCR-based methods, are available but prone to false negative results. CASE PRESENTATION: This report included five cases of Chlamydia psittaci pneumonia. Symptoms and signs common to all 5 cases included fever, coughing, generalized muscle ache, and most notably, inflammatory infiltration of the lungs upon chest CT and X-ray. Metagenomic next-generation sequencing (mNGS) revealed the presence of Chlamydia psittaci in biopsy lung tissue in 3 cases and bronchoalveolar lavage fluid in the remaining 2 cases. Three patients responded to doxycycline plus moxifloxacin; two patients responded to moxifloxacin alone. CONCLUSIONS: mNGS could be used to diagnose Chlamydia psittaci pneumonia.
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spelling pubmed-70771292020-03-19 The application of metagenomic next-generation sequencing in diagnosing Chlamydia psittaci pneumonia: a report of five cases Gu, Lei Liu, Wei Ru, Meng Lin, Jing Yu, Guoqing Ye, Jia Zhu, Zheng-an Liu, Yuebin Chen, Jian Lai, Guoxiang Wen, Wen BMC Pulm Med Case Report BACKGROUND: Chlamydia psittaci pneumonia is a zoonotic infectious disease caused by Chlamydia psittaci. Diagnostic tools, including culture, serologic test and PCR-based methods, are available but prone to false negative results. CASE PRESENTATION: This report included five cases of Chlamydia psittaci pneumonia. Symptoms and signs common to all 5 cases included fever, coughing, generalized muscle ache, and most notably, inflammatory infiltration of the lungs upon chest CT and X-ray. Metagenomic next-generation sequencing (mNGS) revealed the presence of Chlamydia psittaci in biopsy lung tissue in 3 cases and bronchoalveolar lavage fluid in the remaining 2 cases. Three patients responded to doxycycline plus moxifloxacin; two patients responded to moxifloxacin alone. CONCLUSIONS: mNGS could be used to diagnose Chlamydia psittaci pneumonia. BioMed Central 2020-03-17 /pmc/articles/PMC7077129/ /pubmed/32178660 http://dx.doi.org/10.1186/s12890-020-1098-x Text en © The Author(s). 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Case Report
Gu, Lei
Liu, Wei
Ru, Meng
Lin, Jing
Yu, Guoqing
Ye, Jia
Zhu, Zheng-an
Liu, Yuebin
Chen, Jian
Lai, Guoxiang
Wen, Wen
The application of metagenomic next-generation sequencing in diagnosing Chlamydia psittaci pneumonia: a report of five cases
title The application of metagenomic next-generation sequencing in diagnosing Chlamydia psittaci pneumonia: a report of five cases
title_full The application of metagenomic next-generation sequencing in diagnosing Chlamydia psittaci pneumonia: a report of five cases
title_fullStr The application of metagenomic next-generation sequencing in diagnosing Chlamydia psittaci pneumonia: a report of five cases
title_full_unstemmed The application of metagenomic next-generation sequencing in diagnosing Chlamydia psittaci pneumonia: a report of five cases
title_short The application of metagenomic next-generation sequencing in diagnosing Chlamydia psittaci pneumonia: a report of five cases
title_sort application of metagenomic next-generation sequencing in diagnosing chlamydia psittaci pneumonia: a report of five cases
topic Case Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7077129/
https://www.ncbi.nlm.nih.gov/pubmed/32178660
http://dx.doi.org/10.1186/s12890-020-1098-x
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