Molecular Characterization of a Novel Ourmia-Like Virus Infecting Phoma matteucciicola

Here, we report a novel (+) ssRNA mycovirus, Phoma matteucciicola ourmia-like virus 1 (PmOLV1), isolated from Phoma matteucciicola strain LG915-1. The genome of PmOLV1 was 2603 nucleotides long and contained a single open reading frame (ORF), which could be translated into a product of RNA-dependent...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhou, Jia, Wang, Yuhua, Liang, Xiaofei, Xie, Changping, Liu, Wenbo, Miao, Weiguo, Kang, Zhensheng, Zheng, Li
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7077192/
https://www.ncbi.nlm.nih.gov/pubmed/32093074
http://dx.doi.org/10.3390/v12020231
_version_ 1783507376349904896
author Zhou, Jia
Wang, Yuhua
Liang, Xiaofei
Xie, Changping
Liu, Wenbo
Miao, Weiguo
Kang, Zhensheng
Zheng, Li
author_facet Zhou, Jia
Wang, Yuhua
Liang, Xiaofei
Xie, Changping
Liu, Wenbo
Miao, Weiguo
Kang, Zhensheng
Zheng, Li
author_sort Zhou, Jia
collection PubMed
description Here, we report a novel (+) ssRNA mycovirus, Phoma matteucciicola ourmia-like virus 1 (PmOLV1), isolated from Phoma matteucciicola strain LG915-1. The genome of PmOLV1 was 2603 nucleotides long and contained a single open reading frame (ORF), which could be translated into a product of RNA-dependent RNA polymerase (RdRp) by both standard and mitochondrial genetic codons. Cellular fractionation assay indicated that PmOLV1 RNAs are likely more enriched in mitochondria than in cytoplasm. Phylogenetic analysis indicated that PmOLV1 is a new member of the genus Penoulivirus (recently proposed) within the family Botourmiaviridae.
format Online
Article
Text
id pubmed-7077192
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-70771922020-03-20 Molecular Characterization of a Novel Ourmia-Like Virus Infecting Phoma matteucciicola Zhou, Jia Wang, Yuhua Liang, Xiaofei Xie, Changping Liu, Wenbo Miao, Weiguo Kang, Zhensheng Zheng, Li Viruses Communication Here, we report a novel (+) ssRNA mycovirus, Phoma matteucciicola ourmia-like virus 1 (PmOLV1), isolated from Phoma matteucciicola strain LG915-1. The genome of PmOLV1 was 2603 nucleotides long and contained a single open reading frame (ORF), which could be translated into a product of RNA-dependent RNA polymerase (RdRp) by both standard and mitochondrial genetic codons. Cellular fractionation assay indicated that PmOLV1 RNAs are likely more enriched in mitochondria than in cytoplasm. Phylogenetic analysis indicated that PmOLV1 is a new member of the genus Penoulivirus (recently proposed) within the family Botourmiaviridae. MDPI 2020-02-19 /pmc/articles/PMC7077192/ /pubmed/32093074 http://dx.doi.org/10.3390/v12020231 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Zhou, Jia
Wang, Yuhua
Liang, Xiaofei
Xie, Changping
Liu, Wenbo
Miao, Weiguo
Kang, Zhensheng
Zheng, Li
Molecular Characterization of a Novel Ourmia-Like Virus Infecting Phoma matteucciicola
title Molecular Characterization of a Novel Ourmia-Like Virus Infecting Phoma matteucciicola
title_full Molecular Characterization of a Novel Ourmia-Like Virus Infecting Phoma matteucciicola
title_fullStr Molecular Characterization of a Novel Ourmia-Like Virus Infecting Phoma matteucciicola
title_full_unstemmed Molecular Characterization of a Novel Ourmia-Like Virus Infecting Phoma matteucciicola
title_short Molecular Characterization of a Novel Ourmia-Like Virus Infecting Phoma matteucciicola
title_sort molecular characterization of a novel ourmia-like virus infecting phoma matteucciicola
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7077192/
https://www.ncbi.nlm.nih.gov/pubmed/32093074
http://dx.doi.org/10.3390/v12020231
work_keys_str_mv AT zhoujia molecularcharacterizationofanovelourmialikevirusinfectingphomamatteucciicola
AT wangyuhua molecularcharacterizationofanovelourmialikevirusinfectingphomamatteucciicola
AT liangxiaofei molecularcharacterizationofanovelourmialikevirusinfectingphomamatteucciicola
AT xiechangping molecularcharacterizationofanovelourmialikevirusinfectingphomamatteucciicola
AT liuwenbo molecularcharacterizationofanovelourmialikevirusinfectingphomamatteucciicola
AT miaoweiguo molecularcharacterizationofanovelourmialikevirusinfectingphomamatteucciicola
AT kangzhensheng molecularcharacterizationofanovelourmialikevirusinfectingphomamatteucciicola
AT zhengli molecularcharacterizationofanovelourmialikevirusinfectingphomamatteucciicola

Ejemplares similares