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The First Non-LRV RNA Virus in Leishmania
In this work, we describe the first Leishmania-infecting leishbunyavirus—the first virus other than Leishmania RNA virus (LRV) found in trypanosomatid parasites. Its host is Leishmania martiniquensis, a human pathogen causing infections with a wide range of manifestations from asymptomatic to severe...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7077295/ https://www.ncbi.nlm.nih.gov/pubmed/32024293 http://dx.doi.org/10.3390/v12020168 |
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author | Grybchuk, Danyil Macedo, Diego H. Kleschenko, Yulia Kraeva, Natalya Lukashev, Alexander N. Bates, Paul A. Kulich, Pavel Leštinová, Tereza Volf, Petr Kostygov, Alexei Y. Yurchenko, Vyacheslav |
author_facet | Grybchuk, Danyil Macedo, Diego H. Kleschenko, Yulia Kraeva, Natalya Lukashev, Alexander N. Bates, Paul A. Kulich, Pavel Leštinová, Tereza Volf, Petr Kostygov, Alexei Y. Yurchenko, Vyacheslav |
author_sort | Grybchuk, Danyil |
collection | PubMed |
description | In this work, we describe the first Leishmania-infecting leishbunyavirus—the first virus other than Leishmania RNA virus (LRV) found in trypanosomatid parasites. Its host is Leishmania martiniquensis, a human pathogen causing infections with a wide range of manifestations from asymptomatic to severe visceral disease. This virus (LmarLBV1) possesses many characteristic features of leishbunyaviruses, such as tripartite organization of its RNA genome, with ORFs encoding RNA-dependent RNA polymerase, surface glycoprotein, and nucleoprotein on L, M, and S segments, respectively. Our phylogenetic analyses suggest that LmarLBV1 originated from leishbunyaviruses of monoxenous trypanosomatids and, probably, is a result of genomic re-assortment. The LmarLBV1 facilitates parasites’ infectivity in vitro in primary murine macrophages model. The discovery of a virus in L. martiniquensis poses the question of whether it influences pathogenicity of this parasite in vivo, similarly to the LRV in other Leishmania species. |
format | Online Article Text |
id | pubmed-7077295 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-70772952020-03-20 The First Non-LRV RNA Virus in Leishmania Grybchuk, Danyil Macedo, Diego H. Kleschenko, Yulia Kraeva, Natalya Lukashev, Alexander N. Bates, Paul A. Kulich, Pavel Leštinová, Tereza Volf, Petr Kostygov, Alexei Y. Yurchenko, Vyacheslav Viruses Article In this work, we describe the first Leishmania-infecting leishbunyavirus—the first virus other than Leishmania RNA virus (LRV) found in trypanosomatid parasites. Its host is Leishmania martiniquensis, a human pathogen causing infections with a wide range of manifestations from asymptomatic to severe visceral disease. This virus (LmarLBV1) possesses many characteristic features of leishbunyaviruses, such as tripartite organization of its RNA genome, with ORFs encoding RNA-dependent RNA polymerase, surface glycoprotein, and nucleoprotein on L, M, and S segments, respectively. Our phylogenetic analyses suggest that LmarLBV1 originated from leishbunyaviruses of monoxenous trypanosomatids and, probably, is a result of genomic re-assortment. The LmarLBV1 facilitates parasites’ infectivity in vitro in primary murine macrophages model. The discovery of a virus in L. martiniquensis poses the question of whether it influences pathogenicity of this parasite in vivo, similarly to the LRV in other Leishmania species. MDPI 2020-02-02 /pmc/articles/PMC7077295/ /pubmed/32024293 http://dx.doi.org/10.3390/v12020168 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Grybchuk, Danyil Macedo, Diego H. Kleschenko, Yulia Kraeva, Natalya Lukashev, Alexander N. Bates, Paul A. Kulich, Pavel Leštinová, Tereza Volf, Petr Kostygov, Alexei Y. Yurchenko, Vyacheslav The First Non-LRV RNA Virus in Leishmania |
title | The First Non-LRV RNA Virus in Leishmania |
title_full | The First Non-LRV RNA Virus in Leishmania |
title_fullStr | The First Non-LRV RNA Virus in Leishmania |
title_full_unstemmed | The First Non-LRV RNA Virus in Leishmania |
title_short | The First Non-LRV RNA Virus in Leishmania |
title_sort | first non-lrv rna virus in leishmania |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7077295/ https://www.ncbi.nlm.nih.gov/pubmed/32024293 http://dx.doi.org/10.3390/v12020168 |
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