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ABCE1 Acts as a Positive Regulator of Exogenous RNA Decay
The 2′-5′-oligoadenylate synthetase (OAS)/RNase L system protects hosts against pathogenic viruses through cleavage of the exogenous single-stranded RNA. In this system, an evolutionally conserved RNA quality control factor Dom34 (known as Pelota (Pelo) in higher eukaryotes) forms a surveillance com...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7077301/ https://www.ncbi.nlm.nih.gov/pubmed/32033097 http://dx.doi.org/10.3390/v12020174 |
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author | Nogimori, Takuto Ogami, Koichi Oishi, Yuka Goda, Ryoya Hosoda, Nao Kitamura, Yoshiaki Kitade, Yukio Hoshino, Shin-ichi |
author_facet | Nogimori, Takuto Ogami, Koichi Oishi, Yuka Goda, Ryoya Hosoda, Nao Kitamura, Yoshiaki Kitade, Yukio Hoshino, Shin-ichi |
author_sort | Nogimori, Takuto |
collection | PubMed |
description | The 2′-5′-oligoadenylate synthetase (OAS)/RNase L system protects hosts against pathogenic viruses through cleavage of the exogenous single-stranded RNA. In this system, an evolutionally conserved RNA quality control factor Dom34 (known as Pelota (Pelo) in higher eukaryotes) forms a surveillance complex with RNase L to recognize and eliminate the exogenous RNA in a manner dependent on translation. Here, we newly identified that ATP-binding cassette sub-family E member 1 (ABCE1), which is also known as RNase L inhibitor (RLI), is involved in the regulation of exogenous RNA decay. ABCE1 directly binds to form a complex with RNase L and accelerates RNase L dimer formation in the absence of 2′-5′ oligoadenylates (2-5A). Depletion of ABCE1 represses 2-5A-induced RNase L activation and stabilizes exogenous RNA to a level comparable to that seen in RNase L depletion. The increased half-life of the RNA by the single depletion of either protein is not significantly affected by the double depletion of both proteins, suggesting that RNase L and ABCE1 act together to eliminate exogenous RNA. Our results indicate that ABCE1 functions as a positive regulator of exogenous RNA decay rather than an inhibitor of RNase L. |
format | Online Article Text |
id | pubmed-7077301 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-70773012020-03-20 ABCE1 Acts as a Positive Regulator of Exogenous RNA Decay Nogimori, Takuto Ogami, Koichi Oishi, Yuka Goda, Ryoya Hosoda, Nao Kitamura, Yoshiaki Kitade, Yukio Hoshino, Shin-ichi Viruses Communication The 2′-5′-oligoadenylate synthetase (OAS)/RNase L system protects hosts against pathogenic viruses through cleavage of the exogenous single-stranded RNA. In this system, an evolutionally conserved RNA quality control factor Dom34 (known as Pelota (Pelo) in higher eukaryotes) forms a surveillance complex with RNase L to recognize and eliminate the exogenous RNA in a manner dependent on translation. Here, we newly identified that ATP-binding cassette sub-family E member 1 (ABCE1), which is also known as RNase L inhibitor (RLI), is involved in the regulation of exogenous RNA decay. ABCE1 directly binds to form a complex with RNase L and accelerates RNase L dimer formation in the absence of 2′-5′ oligoadenylates (2-5A). Depletion of ABCE1 represses 2-5A-induced RNase L activation and stabilizes exogenous RNA to a level comparable to that seen in RNase L depletion. The increased half-life of the RNA by the single depletion of either protein is not significantly affected by the double depletion of both proteins, suggesting that RNase L and ABCE1 act together to eliminate exogenous RNA. Our results indicate that ABCE1 functions as a positive regulator of exogenous RNA decay rather than an inhibitor of RNase L. MDPI 2020-02-04 /pmc/articles/PMC7077301/ /pubmed/32033097 http://dx.doi.org/10.3390/v12020174 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Nogimori, Takuto Ogami, Koichi Oishi, Yuka Goda, Ryoya Hosoda, Nao Kitamura, Yoshiaki Kitade, Yukio Hoshino, Shin-ichi ABCE1 Acts as a Positive Regulator of Exogenous RNA Decay |
title | ABCE1 Acts as a Positive Regulator of Exogenous RNA Decay |
title_full | ABCE1 Acts as a Positive Regulator of Exogenous RNA Decay |
title_fullStr | ABCE1 Acts as a Positive Regulator of Exogenous RNA Decay |
title_full_unstemmed | ABCE1 Acts as a Positive Regulator of Exogenous RNA Decay |
title_short | ABCE1 Acts as a Positive Regulator of Exogenous RNA Decay |
title_sort | abce1 acts as a positive regulator of exogenous rna decay |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7077301/ https://www.ncbi.nlm.nih.gov/pubmed/32033097 http://dx.doi.org/10.3390/v12020174 |
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