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ABCE1 Acts as a Positive Regulator of Exogenous RNA Decay

The 2′-5′-oligoadenylate synthetase (OAS)/RNase L system protects hosts against pathogenic viruses through cleavage of the exogenous single-stranded RNA. In this system, an evolutionally conserved RNA quality control factor Dom34 (known as Pelota (Pelo) in higher eukaryotes) forms a surveillance com...

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Autores principales: Nogimori, Takuto, Ogami, Koichi, Oishi, Yuka, Goda, Ryoya, Hosoda, Nao, Kitamura, Yoshiaki, Kitade, Yukio, Hoshino, Shin-ichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7077301/
https://www.ncbi.nlm.nih.gov/pubmed/32033097
http://dx.doi.org/10.3390/v12020174
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author Nogimori, Takuto
Ogami, Koichi
Oishi, Yuka
Goda, Ryoya
Hosoda, Nao
Kitamura, Yoshiaki
Kitade, Yukio
Hoshino, Shin-ichi
author_facet Nogimori, Takuto
Ogami, Koichi
Oishi, Yuka
Goda, Ryoya
Hosoda, Nao
Kitamura, Yoshiaki
Kitade, Yukio
Hoshino, Shin-ichi
author_sort Nogimori, Takuto
collection PubMed
description The 2′-5′-oligoadenylate synthetase (OAS)/RNase L system protects hosts against pathogenic viruses through cleavage of the exogenous single-stranded RNA. In this system, an evolutionally conserved RNA quality control factor Dom34 (known as Pelota (Pelo) in higher eukaryotes) forms a surveillance complex with RNase L to recognize and eliminate the exogenous RNA in a manner dependent on translation. Here, we newly identified that ATP-binding cassette sub-family E member 1 (ABCE1), which is also known as RNase L inhibitor (RLI), is involved in the regulation of exogenous RNA decay. ABCE1 directly binds to form a complex with RNase L and accelerates RNase L dimer formation in the absence of 2′-5′ oligoadenylates (2-5A). Depletion of ABCE1 represses 2-5A-induced RNase L activation and stabilizes exogenous RNA to a level comparable to that seen in RNase L depletion. The increased half-life of the RNA by the single depletion of either protein is not significantly affected by the double depletion of both proteins, suggesting that RNase L and ABCE1 act together to eliminate exogenous RNA. Our results indicate that ABCE1 functions as a positive regulator of exogenous RNA decay rather than an inhibitor of RNase L.
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spelling pubmed-70773012020-03-20 ABCE1 Acts as a Positive Regulator of Exogenous RNA Decay Nogimori, Takuto Ogami, Koichi Oishi, Yuka Goda, Ryoya Hosoda, Nao Kitamura, Yoshiaki Kitade, Yukio Hoshino, Shin-ichi Viruses Communication The 2′-5′-oligoadenylate synthetase (OAS)/RNase L system protects hosts against pathogenic viruses through cleavage of the exogenous single-stranded RNA. In this system, an evolutionally conserved RNA quality control factor Dom34 (known as Pelota (Pelo) in higher eukaryotes) forms a surveillance complex with RNase L to recognize and eliminate the exogenous RNA in a manner dependent on translation. Here, we newly identified that ATP-binding cassette sub-family E member 1 (ABCE1), which is also known as RNase L inhibitor (RLI), is involved in the regulation of exogenous RNA decay. ABCE1 directly binds to form a complex with RNase L and accelerates RNase L dimer formation in the absence of 2′-5′ oligoadenylates (2-5A). Depletion of ABCE1 represses 2-5A-induced RNase L activation and stabilizes exogenous RNA to a level comparable to that seen in RNase L depletion. The increased half-life of the RNA by the single depletion of either protein is not significantly affected by the double depletion of both proteins, suggesting that RNase L and ABCE1 act together to eliminate exogenous RNA. Our results indicate that ABCE1 functions as a positive regulator of exogenous RNA decay rather than an inhibitor of RNase L. MDPI 2020-02-04 /pmc/articles/PMC7077301/ /pubmed/32033097 http://dx.doi.org/10.3390/v12020174 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Nogimori, Takuto
Ogami, Koichi
Oishi, Yuka
Goda, Ryoya
Hosoda, Nao
Kitamura, Yoshiaki
Kitade, Yukio
Hoshino, Shin-ichi
ABCE1 Acts as a Positive Regulator of Exogenous RNA Decay
title ABCE1 Acts as a Positive Regulator of Exogenous RNA Decay
title_full ABCE1 Acts as a Positive Regulator of Exogenous RNA Decay
title_fullStr ABCE1 Acts as a Positive Regulator of Exogenous RNA Decay
title_full_unstemmed ABCE1 Acts as a Positive Regulator of Exogenous RNA Decay
title_short ABCE1 Acts as a Positive Regulator of Exogenous RNA Decay
title_sort abce1 acts as a positive regulator of exogenous rna decay
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7077301/
https://www.ncbi.nlm.nih.gov/pubmed/32033097
http://dx.doi.org/10.3390/v12020174
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