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MicroRNA-7 Inhibits Rotavirus Replication by Targeting Viral NSP5 In Vivo and In Vitro

Rotavirus (RV) is the major causes of severe diarrhea in infants and young children under five years of age. There are no effective drugs for the treatment of rotavirus in addition to preventive live attenuated vaccine. Recent evidence demonstrates that microRNAs (miRNAs) can affect RNA virus replic...

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Autores principales: Zhou, Yan, Chen, Linlin, Du, Jing, Hu, Xiaoqing, Xie, Yuping, Wu, Jinyuan, Lin, Xiaochen, Yin, Na, Sun, Maosheng, Li, Hongjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7077326/
https://www.ncbi.nlm.nih.gov/pubmed/32069901
http://dx.doi.org/10.3390/v12020209
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author Zhou, Yan
Chen, Linlin
Du, Jing
Hu, Xiaoqing
Xie, Yuping
Wu, Jinyuan
Lin, Xiaochen
Yin, Na
Sun, Maosheng
Li, Hongjun
author_facet Zhou, Yan
Chen, Linlin
Du, Jing
Hu, Xiaoqing
Xie, Yuping
Wu, Jinyuan
Lin, Xiaochen
Yin, Na
Sun, Maosheng
Li, Hongjun
author_sort Zhou, Yan
collection PubMed
description Rotavirus (RV) is the major causes of severe diarrhea in infants and young children under five years of age. There are no effective drugs for the treatment of rotavirus in addition to preventive live attenuated vaccine. Recent evidence demonstrates that microRNAs (miRNAs) can affect RNA virus replication. However, the antiviral effect of miRNAs during rotavirus replication are largely unknown. Here, we determined that miR-7 is upregulated during RV replication and that it targets the RV NSP5 (Nonstructural protein 5). Results suggested that miR-7 affected viroplasm formation and inhibited RV replication by down-regulating RV NSP5 expression. Up-regulation of miR-7 expression is a common regulation method of different G-type RV-infected host cells. Then, we further revealed the antiviral effect of miR-7 in diarrhea suckling mice model. MiR-7 is able to inhibit rotavirus replication in vitro and in vivo. These data provide that understanding the role of cellular miR-7 during rotaviral replication may help in the identification of novel therapeutic small RNA molecule drug for anti-rotavirus.
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spelling pubmed-70773262020-03-20 MicroRNA-7 Inhibits Rotavirus Replication by Targeting Viral NSP5 In Vivo and In Vitro Zhou, Yan Chen, Linlin Du, Jing Hu, Xiaoqing Xie, Yuping Wu, Jinyuan Lin, Xiaochen Yin, Na Sun, Maosheng Li, Hongjun Viruses Article Rotavirus (RV) is the major causes of severe diarrhea in infants and young children under five years of age. There are no effective drugs for the treatment of rotavirus in addition to preventive live attenuated vaccine. Recent evidence demonstrates that microRNAs (miRNAs) can affect RNA virus replication. However, the antiviral effect of miRNAs during rotavirus replication are largely unknown. Here, we determined that miR-7 is upregulated during RV replication and that it targets the RV NSP5 (Nonstructural protein 5). Results suggested that miR-7 affected viroplasm formation and inhibited RV replication by down-regulating RV NSP5 expression. Up-regulation of miR-7 expression is a common regulation method of different G-type RV-infected host cells. Then, we further revealed the antiviral effect of miR-7 in diarrhea suckling mice model. MiR-7 is able to inhibit rotavirus replication in vitro and in vivo. These data provide that understanding the role of cellular miR-7 during rotaviral replication may help in the identification of novel therapeutic small RNA molecule drug for anti-rotavirus. MDPI 2020-02-13 /pmc/articles/PMC7077326/ /pubmed/32069901 http://dx.doi.org/10.3390/v12020209 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhou, Yan
Chen, Linlin
Du, Jing
Hu, Xiaoqing
Xie, Yuping
Wu, Jinyuan
Lin, Xiaochen
Yin, Na
Sun, Maosheng
Li, Hongjun
MicroRNA-7 Inhibits Rotavirus Replication by Targeting Viral NSP5 In Vivo and In Vitro
title MicroRNA-7 Inhibits Rotavirus Replication by Targeting Viral NSP5 In Vivo and In Vitro
title_full MicroRNA-7 Inhibits Rotavirus Replication by Targeting Viral NSP5 In Vivo and In Vitro
title_fullStr MicroRNA-7 Inhibits Rotavirus Replication by Targeting Viral NSP5 In Vivo and In Vitro
title_full_unstemmed MicroRNA-7 Inhibits Rotavirus Replication by Targeting Viral NSP5 In Vivo and In Vitro
title_short MicroRNA-7 Inhibits Rotavirus Replication by Targeting Viral NSP5 In Vivo and In Vitro
title_sort microrna-7 inhibits rotavirus replication by targeting viral nsp5 in vivo and in vitro
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7077326/
https://www.ncbi.nlm.nih.gov/pubmed/32069901
http://dx.doi.org/10.3390/v12020209
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