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Characterization of the Wheat-Psathyrostachys huashania Keng 2Ns/2D Substitution Line H139: A Novel Germplasm With Enhanced Resistance to Wheat Take-All

Take-all is a devastating soil-borne disease that affects wheat production. The continuous generation of disease-resistance germplasm is an important aspect of the management of this pathogen. In this study, we characterized the wheat-Psathyrostachys huashania Keng (P. huashania)-derived progeny H13...

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Autores principales: Bai, Shengsheng, Yuan, Fengping, Zhang, Hanbing, Zhang, Zhenyue, Zhao, Jixin, Yang, Qunhui, Wu, Jun, Chen, Xinhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7077511/
https://www.ncbi.nlm.nih.gov/pubmed/32210998
http://dx.doi.org/10.3389/fpls.2020.00233
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author Bai, Shengsheng
Yuan, Fengping
Zhang, Hanbing
Zhang, Zhenyue
Zhao, Jixin
Yang, Qunhui
Wu, Jun
Chen, Xinhong
author_facet Bai, Shengsheng
Yuan, Fengping
Zhang, Hanbing
Zhang, Zhenyue
Zhao, Jixin
Yang, Qunhui
Wu, Jun
Chen, Xinhong
author_sort Bai, Shengsheng
collection PubMed
description Take-all is a devastating soil-borne disease that affects wheat production. The continuous generation of disease-resistance germplasm is an important aspect of the management of this pathogen. In this study, we characterized the wheat-Psathyrostachys huashania Keng (P. huashania)-derived progeny H139 that exhibits significantly improved resistance to wheat take-all disease compared with its susceptible parent 7182. Sequential genomic in situ hybridization (GISH) and multicolor fluorescence in situ hybridization (mc-FISH) analyses revealed that H139 is a stable wheat-P. huashania disomic substitution line lacking wheat chromosome 2D. Expressed sequence tag-sequence tagged site (EST-STS) marker and Wheat Axiom 660K Genotyping Array analysis further revealed that H139 was a novel wheat-P. huashania 2Ns/2D substitution line. In addition, the H139 line was shown to be cytologically stable with a dwarf phenotype and increased spikelet number. These results indicate that H139, with its enhanced wheat take-all disease resistance and desirable agronomic traits, provides valuable genetic resources for wheat chromosome engineering breeding.
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spelling pubmed-70775112020-03-24 Characterization of the Wheat-Psathyrostachys huashania Keng 2Ns/2D Substitution Line H139: A Novel Germplasm With Enhanced Resistance to Wheat Take-All Bai, Shengsheng Yuan, Fengping Zhang, Hanbing Zhang, Zhenyue Zhao, Jixin Yang, Qunhui Wu, Jun Chen, Xinhong Front Plant Sci Plant Science Take-all is a devastating soil-borne disease that affects wheat production. The continuous generation of disease-resistance germplasm is an important aspect of the management of this pathogen. In this study, we characterized the wheat-Psathyrostachys huashania Keng (P. huashania)-derived progeny H139 that exhibits significantly improved resistance to wheat take-all disease compared with its susceptible parent 7182. Sequential genomic in situ hybridization (GISH) and multicolor fluorescence in situ hybridization (mc-FISH) analyses revealed that H139 is a stable wheat-P. huashania disomic substitution line lacking wheat chromosome 2D. Expressed sequence tag-sequence tagged site (EST-STS) marker and Wheat Axiom 660K Genotyping Array analysis further revealed that H139 was a novel wheat-P. huashania 2Ns/2D substitution line. In addition, the H139 line was shown to be cytologically stable with a dwarf phenotype and increased spikelet number. These results indicate that H139, with its enhanced wheat take-all disease resistance and desirable agronomic traits, provides valuable genetic resources for wheat chromosome engineering breeding. Frontiers Media S.A. 2020-03-10 /pmc/articles/PMC7077511/ /pubmed/32210998 http://dx.doi.org/10.3389/fpls.2020.00233 Text en Copyright © 2020 Bai, Yuan, Zhang, Zhang, Zhao, Yang, Wu and Chen. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Bai, Shengsheng
Yuan, Fengping
Zhang, Hanbing
Zhang, Zhenyue
Zhao, Jixin
Yang, Qunhui
Wu, Jun
Chen, Xinhong
Characterization of the Wheat-Psathyrostachys huashania Keng 2Ns/2D Substitution Line H139: A Novel Germplasm With Enhanced Resistance to Wheat Take-All
title Characterization of the Wheat-Psathyrostachys huashania Keng 2Ns/2D Substitution Line H139: A Novel Germplasm With Enhanced Resistance to Wheat Take-All
title_full Characterization of the Wheat-Psathyrostachys huashania Keng 2Ns/2D Substitution Line H139: A Novel Germplasm With Enhanced Resistance to Wheat Take-All
title_fullStr Characterization of the Wheat-Psathyrostachys huashania Keng 2Ns/2D Substitution Line H139: A Novel Germplasm With Enhanced Resistance to Wheat Take-All
title_full_unstemmed Characterization of the Wheat-Psathyrostachys huashania Keng 2Ns/2D Substitution Line H139: A Novel Germplasm With Enhanced Resistance to Wheat Take-All
title_short Characterization of the Wheat-Psathyrostachys huashania Keng 2Ns/2D Substitution Line H139: A Novel Germplasm With Enhanced Resistance to Wheat Take-All
title_sort characterization of the wheat-psathyrostachys huashania keng 2ns/2d substitution line h139: a novel germplasm with enhanced resistance to wheat take-all
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7077511/
https://www.ncbi.nlm.nih.gov/pubmed/32210998
http://dx.doi.org/10.3389/fpls.2020.00233
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