Cargando…
Evaluation of burn wound healing activity of novel fusidic acid loaded microemulsion based gel in male Wistar albino rats
The objective of the present research was to examine the possible usage of microemulsion based gel for fusidic acid (FA) dermal application as burn wound treatment. During the preparation of microemulsion, ethyl oleate as oil phase, tween 80 as a surfactant, ethanol as co-surfactant, water as aqueou...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7078556/ https://www.ncbi.nlm.nih.gov/pubmed/32194336 http://dx.doi.org/10.1016/j.jsps.2020.01.015 |
_version_ | 1783507644729786368 |
---|---|
author | Okur, Mehmet Evren Ayla, Şule Yozgatlı, Vildan Aksu, Neşe Buket Yoltaş, Ayşegül Orak, Duygu Sipahi, Hande Üstündağ Okur, Neslihan |
author_facet | Okur, Mehmet Evren Ayla, Şule Yozgatlı, Vildan Aksu, Neşe Buket Yoltaş, Ayşegül Orak, Duygu Sipahi, Hande Üstündağ Okur, Neslihan |
author_sort | Okur, Mehmet Evren |
collection | PubMed |
description | The objective of the present research was to examine the possible usage of microemulsion based gel for fusidic acid (FA) dermal application as burn wound treatment. During the preparation of microemulsion, ethyl oleate as oil phase, tween 80 as a surfactant, ethanol as co-surfactant, water as aqueous phase were used. The prepared microemulsions were evaluated for clarity, pH, viscosity and FA content. Moreover, stability, sterility, antibacterial activity, in vitro release of the formulations were also evaluated. The results showed that the FA loaded microemulsion and microemulsion based gel formation and characteristics were related to many parameters of the components. The performed optimized microemulsion-based gel showed good stability over a period of 3 months. The antibacterial activity of microemulsion-based gel was found to be comparable with marketed cream. RAW 264.7 macrophages were used to determine cell viability (MTT assay) and nitric oxide production. MBG and FA-MBG significantly inhibit the production of the inflammatory mediator NO in LPS-stimulated RAW 264.7 cells in a concentration-dependent manner. The wound healing property was evaluated by histopathological examination and by measuring the wound contraction. The % of wound area in rats treated with FA (2%) loaded microemulsion based gel ranged from 69.30% to 41.39% in the period from 3 to 10 days. In conclusion, FA loaded microemulsion based gel could be offered as encouraging strategy as dermal systems for the burn wound treatment. |
format | Online Article Text |
id | pubmed-7078556 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-70785562020-03-19 Evaluation of burn wound healing activity of novel fusidic acid loaded microemulsion based gel in male Wistar albino rats Okur, Mehmet Evren Ayla, Şule Yozgatlı, Vildan Aksu, Neşe Buket Yoltaş, Ayşegül Orak, Duygu Sipahi, Hande Üstündağ Okur, Neslihan Saudi Pharm J Article The objective of the present research was to examine the possible usage of microemulsion based gel for fusidic acid (FA) dermal application as burn wound treatment. During the preparation of microemulsion, ethyl oleate as oil phase, tween 80 as a surfactant, ethanol as co-surfactant, water as aqueous phase were used. The prepared microemulsions were evaluated for clarity, pH, viscosity and FA content. Moreover, stability, sterility, antibacterial activity, in vitro release of the formulations were also evaluated. The results showed that the FA loaded microemulsion and microemulsion based gel formation and characteristics were related to many parameters of the components. The performed optimized microemulsion-based gel showed good stability over a period of 3 months. The antibacterial activity of microemulsion-based gel was found to be comparable with marketed cream. RAW 264.7 macrophages were used to determine cell viability (MTT assay) and nitric oxide production. MBG and FA-MBG significantly inhibit the production of the inflammatory mediator NO in LPS-stimulated RAW 264.7 cells in a concentration-dependent manner. The wound healing property was evaluated by histopathological examination and by measuring the wound contraction. The % of wound area in rats treated with FA (2%) loaded microemulsion based gel ranged from 69.30% to 41.39% in the period from 3 to 10 days. In conclusion, FA loaded microemulsion based gel could be offered as encouraging strategy as dermal systems for the burn wound treatment. Elsevier 2020-03 2020-02-03 /pmc/articles/PMC7078556/ /pubmed/32194336 http://dx.doi.org/10.1016/j.jsps.2020.01.015 Text en © 2020 The Authors. Published by Elsevier B.V. on behalf of King Saud University. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Okur, Mehmet Evren Ayla, Şule Yozgatlı, Vildan Aksu, Neşe Buket Yoltaş, Ayşegül Orak, Duygu Sipahi, Hande Üstündağ Okur, Neslihan Evaluation of burn wound healing activity of novel fusidic acid loaded microemulsion based gel in male Wistar albino rats |
title | Evaluation of burn wound healing activity of novel fusidic acid loaded microemulsion based gel in male Wistar albino rats |
title_full | Evaluation of burn wound healing activity of novel fusidic acid loaded microemulsion based gel in male Wistar albino rats |
title_fullStr | Evaluation of burn wound healing activity of novel fusidic acid loaded microemulsion based gel in male Wistar albino rats |
title_full_unstemmed | Evaluation of burn wound healing activity of novel fusidic acid loaded microemulsion based gel in male Wistar albino rats |
title_short | Evaluation of burn wound healing activity of novel fusidic acid loaded microemulsion based gel in male Wistar albino rats |
title_sort | evaluation of burn wound healing activity of novel fusidic acid loaded microemulsion based gel in male wistar albino rats |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7078556/ https://www.ncbi.nlm.nih.gov/pubmed/32194336 http://dx.doi.org/10.1016/j.jsps.2020.01.015 |
work_keys_str_mv | AT okurmehmetevren evaluationofburnwoundhealingactivityofnovelfusidicacidloadedmicroemulsionbasedgelinmalewistaralbinorats AT aylasule evaluationofburnwoundhealingactivityofnovelfusidicacidloadedmicroemulsionbasedgelinmalewistaralbinorats AT yozgatlıvildan evaluationofburnwoundhealingactivityofnovelfusidicacidloadedmicroemulsionbasedgelinmalewistaralbinorats AT aksunesebuket evaluationofburnwoundhealingactivityofnovelfusidicacidloadedmicroemulsionbasedgelinmalewistaralbinorats AT yoltasaysegul evaluationofburnwoundhealingactivityofnovelfusidicacidloadedmicroemulsionbasedgelinmalewistaralbinorats AT orakduygu evaluationofburnwoundhealingactivityofnovelfusidicacidloadedmicroemulsionbasedgelinmalewistaralbinorats AT sipahihande evaluationofburnwoundhealingactivityofnovelfusidicacidloadedmicroemulsionbasedgelinmalewistaralbinorats AT ustundagokurneslihan evaluationofburnwoundhealingactivityofnovelfusidicacidloadedmicroemulsionbasedgelinmalewistaralbinorats |