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Evaluation of burn wound healing activity of novel fusidic acid loaded microemulsion based gel in male Wistar albino rats

The objective of the present research was to examine the possible usage of microemulsion based gel for fusidic acid (FA) dermal application as burn wound treatment. During the preparation of microemulsion, ethyl oleate as oil phase, tween 80 as a surfactant, ethanol as co-surfactant, water as aqueou...

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Autores principales: Okur, Mehmet Evren, Ayla, Şule, Yozgatlı, Vildan, Aksu, Neşe Buket, Yoltaş, Ayşegül, Orak, Duygu, Sipahi, Hande, Üstündağ Okur, Neslihan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7078556/
https://www.ncbi.nlm.nih.gov/pubmed/32194336
http://dx.doi.org/10.1016/j.jsps.2020.01.015
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author Okur, Mehmet Evren
Ayla, Şule
Yozgatlı, Vildan
Aksu, Neşe Buket
Yoltaş, Ayşegül
Orak, Duygu
Sipahi, Hande
Üstündağ Okur, Neslihan
author_facet Okur, Mehmet Evren
Ayla, Şule
Yozgatlı, Vildan
Aksu, Neşe Buket
Yoltaş, Ayşegül
Orak, Duygu
Sipahi, Hande
Üstündağ Okur, Neslihan
author_sort Okur, Mehmet Evren
collection PubMed
description The objective of the present research was to examine the possible usage of microemulsion based gel for fusidic acid (FA) dermal application as burn wound treatment. During the preparation of microemulsion, ethyl oleate as oil phase, tween 80 as a surfactant, ethanol as co-surfactant, water as aqueous phase were used. The prepared microemulsions were evaluated for clarity, pH, viscosity and FA content. Moreover, stability, sterility, antibacterial activity, in vitro release of the formulations were also evaluated. The results showed that the FA loaded microemulsion and microemulsion based gel formation and characteristics were related to many parameters of the components. The performed optimized microemulsion-based gel showed good stability over a period of 3 months. The antibacterial activity of microemulsion-based gel was found to be comparable with marketed cream. RAW 264.7 macrophages were used to determine cell viability (MTT assay) and nitric oxide production. MBG and FA-MBG significantly inhibit the production of the inflammatory mediator NO in LPS-stimulated RAW 264.7 cells in a concentration-dependent manner. The wound healing property was evaluated by histopathological examination and by measuring the wound contraction. The % of wound area in rats treated with FA (2%) loaded microemulsion based gel ranged from 69.30% to 41.39% in the period from 3 to 10 days. In conclusion, FA loaded microemulsion based gel could be offered as encouraging strategy as dermal systems for the burn wound treatment.
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spelling pubmed-70785562020-03-19 Evaluation of burn wound healing activity of novel fusidic acid loaded microemulsion based gel in male Wistar albino rats Okur, Mehmet Evren Ayla, Şule Yozgatlı, Vildan Aksu, Neşe Buket Yoltaş, Ayşegül Orak, Duygu Sipahi, Hande Üstündağ Okur, Neslihan Saudi Pharm J Article The objective of the present research was to examine the possible usage of microemulsion based gel for fusidic acid (FA) dermal application as burn wound treatment. During the preparation of microemulsion, ethyl oleate as oil phase, tween 80 as a surfactant, ethanol as co-surfactant, water as aqueous phase were used. The prepared microemulsions were evaluated for clarity, pH, viscosity and FA content. Moreover, stability, sterility, antibacterial activity, in vitro release of the formulations were also evaluated. The results showed that the FA loaded microemulsion and microemulsion based gel formation and characteristics were related to many parameters of the components. The performed optimized microemulsion-based gel showed good stability over a period of 3 months. The antibacterial activity of microemulsion-based gel was found to be comparable with marketed cream. RAW 264.7 macrophages were used to determine cell viability (MTT assay) and nitric oxide production. MBG and FA-MBG significantly inhibit the production of the inflammatory mediator NO in LPS-stimulated RAW 264.7 cells in a concentration-dependent manner. The wound healing property was evaluated by histopathological examination and by measuring the wound contraction. The % of wound area in rats treated with FA (2%) loaded microemulsion based gel ranged from 69.30% to 41.39% in the period from 3 to 10 days. In conclusion, FA loaded microemulsion based gel could be offered as encouraging strategy as dermal systems for the burn wound treatment. Elsevier 2020-03 2020-02-03 /pmc/articles/PMC7078556/ /pubmed/32194336 http://dx.doi.org/10.1016/j.jsps.2020.01.015 Text en © 2020 The Authors. Published by Elsevier B.V. on behalf of King Saud University. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Okur, Mehmet Evren
Ayla, Şule
Yozgatlı, Vildan
Aksu, Neşe Buket
Yoltaş, Ayşegül
Orak, Duygu
Sipahi, Hande
Üstündağ Okur, Neslihan
Evaluation of burn wound healing activity of novel fusidic acid loaded microemulsion based gel in male Wistar albino rats
title Evaluation of burn wound healing activity of novel fusidic acid loaded microemulsion based gel in male Wistar albino rats
title_full Evaluation of burn wound healing activity of novel fusidic acid loaded microemulsion based gel in male Wistar albino rats
title_fullStr Evaluation of burn wound healing activity of novel fusidic acid loaded microemulsion based gel in male Wistar albino rats
title_full_unstemmed Evaluation of burn wound healing activity of novel fusidic acid loaded microemulsion based gel in male Wistar albino rats
title_short Evaluation of burn wound healing activity of novel fusidic acid loaded microemulsion based gel in male Wistar albino rats
title_sort evaluation of burn wound healing activity of novel fusidic acid loaded microemulsion based gel in male wistar albino rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7078556/
https://www.ncbi.nlm.nih.gov/pubmed/32194336
http://dx.doi.org/10.1016/j.jsps.2020.01.015
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