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Use of tendon to produce decellularized sheets of mineralized collagen fibrils for bone tissue repair and regeneration

With demand for alternatives to autograft and allograft materials continuing to rise, development of new scaffolds for bone tissue repair and regeneration remains of significant interest. Engineered collagen‐calcium phosphate (CaP) constructs can offer desirable attributes, including absence of fore...

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Autores principales: Grue, Brendan H., Veres, Samuel P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7079042/
https://www.ncbi.nlm.nih.gov/pubmed/31241254
http://dx.doi.org/10.1002/jbm.b.34438
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author Grue, Brendan H.
Veres, Samuel P.
author_facet Grue, Brendan H.
Veres, Samuel P.
author_sort Grue, Brendan H.
collection PubMed
description With demand for alternatives to autograft and allograft materials continuing to rise, development of new scaffolds for bone tissue repair and regeneration remains of significant interest. Engineered collagen‐calcium phosphate (CaP) constructs can offer desirable attributes, including absence of foreign body response and possession of inherent osteogenic potential. Despite their promise, current collagen‐CaP constructs are limited to nonload‐bearing applications. In this article, we describe a process for creating decellularized sheets of highly aligned, natively cross‐linked, and mineralized collagen fibrils, which may be useful for developing multilaminate collagen‐CaP constructs with improved mechanical properties. Decellularized bovine tendons were cryosectioned to produce thin sheets of aligned collagen fibrils. Mineralization of the sheets was then performed using an alternate soaking method incorporating a polymer‐induced liquid precursor (PILP) process to promote intrafibrillar mineralization, along with incorporation of physiologically relevant amounts of citrate, Mg, and carbonate. Characteristics of the produced scaffolds were assessed using energy‐dispersive X‐ray spectroscopy (EDX), scanning electron microscopy (SEM), and transmission electron microscopy (TEM). Scaffolds were also compared with both native bovine cortical bone and pure hydroxyapatite using X‐ray powder diffraction (XRD), and Fourier transform infrared spectroscopy attenuated total reflection (FTIR‐ATR). Structural and chemical analyses show that the scaffold preparation process that we described is successful in creating mineralized collagen sheets, possessing a mineral phase similar to that found in bone as well as a close association between collagen fibrils and mineral plates.
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spelling pubmed-70790422020-03-19 Use of tendon to produce decellularized sheets of mineralized collagen fibrils for bone tissue repair and regeneration Grue, Brendan H. Veres, Samuel P. J Biomed Mater Res B Appl Biomater Original Research Reports With demand for alternatives to autograft and allograft materials continuing to rise, development of new scaffolds for bone tissue repair and regeneration remains of significant interest. Engineered collagen‐calcium phosphate (CaP) constructs can offer desirable attributes, including absence of foreign body response and possession of inherent osteogenic potential. Despite their promise, current collagen‐CaP constructs are limited to nonload‐bearing applications. In this article, we describe a process for creating decellularized sheets of highly aligned, natively cross‐linked, and mineralized collagen fibrils, which may be useful for developing multilaminate collagen‐CaP constructs with improved mechanical properties. Decellularized bovine tendons were cryosectioned to produce thin sheets of aligned collagen fibrils. Mineralization of the sheets was then performed using an alternate soaking method incorporating a polymer‐induced liquid precursor (PILP) process to promote intrafibrillar mineralization, along with incorporation of physiologically relevant amounts of citrate, Mg, and carbonate. Characteristics of the produced scaffolds were assessed using energy‐dispersive X‐ray spectroscopy (EDX), scanning electron microscopy (SEM), and transmission electron microscopy (TEM). Scaffolds were also compared with both native bovine cortical bone and pure hydroxyapatite using X‐ray powder diffraction (XRD), and Fourier transform infrared spectroscopy attenuated total reflection (FTIR‐ATR). Structural and chemical analyses show that the scaffold preparation process that we described is successful in creating mineralized collagen sheets, possessing a mineral phase similar to that found in bone as well as a close association between collagen fibrils and mineral plates. John Wiley & Sons, Inc. 2019-06-26 2020-04 /pmc/articles/PMC7079042/ /pubmed/31241254 http://dx.doi.org/10.1002/jbm.b.34438 Text en © 2019 The Authors. Journal of Biomedical Materials Research Part B: Applied Biomaterials published by Wiley Periodicals, Inc. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Original Research Reports
Grue, Brendan H.
Veres, Samuel P.
Use of tendon to produce decellularized sheets of mineralized collagen fibrils for bone tissue repair and regeneration
title Use of tendon to produce decellularized sheets of mineralized collagen fibrils for bone tissue repair and regeneration
title_full Use of tendon to produce decellularized sheets of mineralized collagen fibrils for bone tissue repair and regeneration
title_fullStr Use of tendon to produce decellularized sheets of mineralized collagen fibrils for bone tissue repair and regeneration
title_full_unstemmed Use of tendon to produce decellularized sheets of mineralized collagen fibrils for bone tissue repair and regeneration
title_short Use of tendon to produce decellularized sheets of mineralized collagen fibrils for bone tissue repair and regeneration
title_sort use of tendon to produce decellularized sheets of mineralized collagen fibrils for bone tissue repair and regeneration
topic Original Research Reports
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7079042/
https://www.ncbi.nlm.nih.gov/pubmed/31241254
http://dx.doi.org/10.1002/jbm.b.34438
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