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Lamprey Parapinopsin (“UVLamP”): a Bistable UV‐Sensitive Optogenetic Switch for Ultrafast Control of GPCR Pathways

Optogenetics uses light‐sensitive proteins, so‐called optogenetic tools, for highly precise spatiotemporal control of cellular states and signals. The major limitations of such tools include the overlap of excitation spectra, phototoxicity, and lack of sensitivity. The protein characterized in this...

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Autores principales: Eickelbeck, Dennis, Rudack, Till, Tennigkeit, Stefan Alexander, Surdin, Tatjana, Karapinar, Raziye, Schwitalla, Jan‐Claudius, Mücher, Brix, Shulman, Maiia, Scherlo, Marvin, Althoff, Philipp, Mark, Melanie D., Gerwert, Klaus, Herlitze, Stefan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7079062/
https://www.ncbi.nlm.nih.gov/pubmed/31468691
http://dx.doi.org/10.1002/cbic.201900485
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author Eickelbeck, Dennis
Rudack, Till
Tennigkeit, Stefan Alexander
Surdin, Tatjana
Karapinar, Raziye
Schwitalla, Jan‐Claudius
Mücher, Brix
Shulman, Maiia
Scherlo, Marvin
Althoff, Philipp
Mark, Melanie D.
Gerwert, Klaus
Herlitze, Stefan
author_facet Eickelbeck, Dennis
Rudack, Till
Tennigkeit, Stefan Alexander
Surdin, Tatjana
Karapinar, Raziye
Schwitalla, Jan‐Claudius
Mücher, Brix
Shulman, Maiia
Scherlo, Marvin
Althoff, Philipp
Mark, Melanie D.
Gerwert, Klaus
Herlitze, Stefan
author_sort Eickelbeck, Dennis
collection PubMed
description Optogenetics uses light‐sensitive proteins, so‐called optogenetic tools, for highly precise spatiotemporal control of cellular states and signals. The major limitations of such tools include the overlap of excitation spectra, phototoxicity, and lack of sensitivity. The protein characterized in this study, the Japanese lamprey parapinopsin, which we named UVLamP, is a promising optogenetic tool to overcome these limitations. Using a hybrid strategy combining molecular, cellular, electrophysiological, and computational methods we elucidated a structural model of the dark state and probed the optogenetic potential of UVLamP. Interestingly, it is the first described bistable vertebrate opsin that has a charged amino acid interacting with the Schiff base in the dark state, that has no relevance for its photoreaction. UVLamP is a bistable UV‐sensitive opsin that allows for precise and sustained optogenetic control of G protein‐coupled receptor (GPCR) pathways and can be switched on, but more importantly also off within milliseconds via lowintensity short light pulses. UVLamP exhibits an extremely narrow excitation spectrum in the UV range allowing for sustained activation of the G(i/o) pathway with a millisecond UV light pulse. Its sustained pathway activation can be switched off, surprisingly also with a millisecond blue light pulse, minimizing phototoxicity. Thus, UVLamP serves as a minimally invasive, narrow‐bandwidth probe for controlling the G(i/o) pathway, allowing for combinatorial use with multiple optogenetic tools or sensors. Because UVLamP activated G(i/o) signals are generally inhibitory and decrease cellular activity, it has tremendous potential for health‐related applications such as relieving pain, blocking seizures, and delaying neurodegeneration.
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spelling pubmed-70790622020-03-19 Lamprey Parapinopsin (“UVLamP”): a Bistable UV‐Sensitive Optogenetic Switch for Ultrafast Control of GPCR Pathways Eickelbeck, Dennis Rudack, Till Tennigkeit, Stefan Alexander Surdin, Tatjana Karapinar, Raziye Schwitalla, Jan‐Claudius Mücher, Brix Shulman, Maiia Scherlo, Marvin Althoff, Philipp Mark, Melanie D. Gerwert, Klaus Herlitze, Stefan Chembiochem Communications Optogenetics uses light‐sensitive proteins, so‐called optogenetic tools, for highly precise spatiotemporal control of cellular states and signals. The major limitations of such tools include the overlap of excitation spectra, phototoxicity, and lack of sensitivity. The protein characterized in this study, the Japanese lamprey parapinopsin, which we named UVLamP, is a promising optogenetic tool to overcome these limitations. Using a hybrid strategy combining molecular, cellular, electrophysiological, and computational methods we elucidated a structural model of the dark state and probed the optogenetic potential of UVLamP. Interestingly, it is the first described bistable vertebrate opsin that has a charged amino acid interacting with the Schiff base in the dark state, that has no relevance for its photoreaction. UVLamP is a bistable UV‐sensitive opsin that allows for precise and sustained optogenetic control of G protein‐coupled receptor (GPCR) pathways and can be switched on, but more importantly also off within milliseconds via lowintensity short light pulses. UVLamP exhibits an extremely narrow excitation spectrum in the UV range allowing for sustained activation of the G(i/o) pathway with a millisecond UV light pulse. Its sustained pathway activation can be switched off, surprisingly also with a millisecond blue light pulse, minimizing phototoxicity. Thus, UVLamP serves as a minimally invasive, narrow‐bandwidth probe for controlling the G(i/o) pathway, allowing for combinatorial use with multiple optogenetic tools or sensors. Because UVLamP activated G(i/o) signals are generally inhibitory and decrease cellular activity, it has tremendous potential for health‐related applications such as relieving pain, blocking seizures, and delaying neurodegeneration. John Wiley and Sons Inc. 2019-10-30 2020-03-02 /pmc/articles/PMC7079062/ /pubmed/31468691 http://dx.doi.org/10.1002/cbic.201900485 Text en © 2019 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Communications
Eickelbeck, Dennis
Rudack, Till
Tennigkeit, Stefan Alexander
Surdin, Tatjana
Karapinar, Raziye
Schwitalla, Jan‐Claudius
Mücher, Brix
Shulman, Maiia
Scherlo, Marvin
Althoff, Philipp
Mark, Melanie D.
Gerwert, Klaus
Herlitze, Stefan
Lamprey Parapinopsin (“UVLamP”): a Bistable UV‐Sensitive Optogenetic Switch for Ultrafast Control of GPCR Pathways
title Lamprey Parapinopsin (“UVLamP”): a Bistable UV‐Sensitive Optogenetic Switch for Ultrafast Control of GPCR Pathways
title_full Lamprey Parapinopsin (“UVLamP”): a Bistable UV‐Sensitive Optogenetic Switch for Ultrafast Control of GPCR Pathways
title_fullStr Lamprey Parapinopsin (“UVLamP”): a Bistable UV‐Sensitive Optogenetic Switch for Ultrafast Control of GPCR Pathways
title_full_unstemmed Lamprey Parapinopsin (“UVLamP”): a Bistable UV‐Sensitive Optogenetic Switch for Ultrafast Control of GPCR Pathways
title_short Lamprey Parapinopsin (“UVLamP”): a Bistable UV‐Sensitive Optogenetic Switch for Ultrafast Control of GPCR Pathways
title_sort lamprey parapinopsin (“uvlamp”): a bistable uv‐sensitive optogenetic switch for ultrafast control of gpcr pathways
topic Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7079062/
https://www.ncbi.nlm.nih.gov/pubmed/31468691
http://dx.doi.org/10.1002/cbic.201900485
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