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Deletion of accessory genes 3a, 3b, 5a or 5b from avian coronavirus infectious bronchitis virus induces an attenuated phenotype both in vitro and in vivo

Avian coronavirus infectious bronchitis virus (IBV) infects domestic fowl, resulting in respiratory disease and causing serious losses in unprotected birds. Its control is mainly achieved by using live attenuated vaccines. Here we explored the possibilities for rationally attenuating IBV to improve...

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Autores principales: Laconi, Andrea, van Beurden, Steven J., Berends, Alinda J., Krämer-Kühl, Annika, Jansen, Christine A., Spekreijse, Dieuwertje, Chénard, Gilles, Philipp, Hans-Christian, Mundt, Egbert, Rottier, Peter J. M., Hélène Verheije, M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Microbiology Society 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7079694/
https://www.ncbi.nlm.nih.gov/pubmed/30067172
http://dx.doi.org/10.1099/jgv.0.001130
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author Laconi, Andrea
van Beurden, Steven J.
Berends, Alinda J.
Krämer-Kühl, Annika
Jansen, Christine A.
Spekreijse, Dieuwertje
Chénard, Gilles
Philipp, Hans-Christian
Mundt, Egbert
Rottier, Peter J. M.
Hélène Verheije, M.
author_facet Laconi, Andrea
van Beurden, Steven J.
Berends, Alinda J.
Krämer-Kühl, Annika
Jansen, Christine A.
Spekreijse, Dieuwertje
Chénard, Gilles
Philipp, Hans-Christian
Mundt, Egbert
Rottier, Peter J. M.
Hélène Verheije, M.
author_sort Laconi, Andrea
collection PubMed
description Avian coronavirus infectious bronchitis virus (IBV) infects domestic fowl, resulting in respiratory disease and causing serious losses in unprotected birds. Its control is mainly achieved by using live attenuated vaccines. Here we explored the possibilities for rationally attenuating IBV to improve our knowledge regarding the function of IBV accessory proteins and for the development of next-generation vaccines with the recently established reverse genetic system for IBV H52 based on targeted RNA recombination and selection of recombinant viruses in embryonated eggs. To this aim, we selectively removed accessory genes 3a, 3b, 5a and 5b individually, and rescued the resulting recombinant (r) rIBV-Δ3a, rIBV-Δ3b, rIBV-Δ5a and rIBV-Δ5b. In vitro inoculation of chicken embryo kidney cells with recombinant and wild-type viruses demonstrated that the accessory protein 5b is involved in the delayed activation of the interferon response of the host after IBV infection. Embryo mortality after the inoculation of 8-day-old embryonated chicken eggs with recombinant and wild-type viruses showed that rIBV-Δ3b, rIBV-Δ5a and rIBV-Δ5b had an attenuated phenotype in ovo, with reduced titres at 6 h p.i. and 12 h p.i. for all viruses, while growing to the same titre as wild-type rIBV at 48 h p.i. When administered to 1-day-old chickens, rIBV-Δ3a, rIBV-Δ3b, rIBV-Δ5a and rIBV-Δ5b showed reduced ciliostasis in comparison to the wild-type viruses. In conclusion, individual deletion of accessory genes in IBV H52 resulted in mutant viruses with an attenuated phenotype.
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spelling pubmed-70796942020-03-19 Deletion of accessory genes 3a, 3b, 5a or 5b from avian coronavirus infectious bronchitis virus induces an attenuated phenotype both in vitro and in vivo Laconi, Andrea van Beurden, Steven J. Berends, Alinda J. Krämer-Kühl, Annika Jansen, Christine A. Spekreijse, Dieuwertje Chénard, Gilles Philipp, Hans-Christian Mundt, Egbert Rottier, Peter J. M. Hélène Verheije, M. J Gen Virol Research Article Avian coronavirus infectious bronchitis virus (IBV) infects domestic fowl, resulting in respiratory disease and causing serious losses in unprotected birds. Its control is mainly achieved by using live attenuated vaccines. Here we explored the possibilities for rationally attenuating IBV to improve our knowledge regarding the function of IBV accessory proteins and for the development of next-generation vaccines with the recently established reverse genetic system for IBV H52 based on targeted RNA recombination and selection of recombinant viruses in embryonated eggs. To this aim, we selectively removed accessory genes 3a, 3b, 5a and 5b individually, and rescued the resulting recombinant (r) rIBV-Δ3a, rIBV-Δ3b, rIBV-Δ5a and rIBV-Δ5b. In vitro inoculation of chicken embryo kidney cells with recombinant and wild-type viruses demonstrated that the accessory protein 5b is involved in the delayed activation of the interferon response of the host after IBV infection. Embryo mortality after the inoculation of 8-day-old embryonated chicken eggs with recombinant and wild-type viruses showed that rIBV-Δ3b, rIBV-Δ5a and rIBV-Δ5b had an attenuated phenotype in ovo, with reduced titres at 6 h p.i. and 12 h p.i. for all viruses, while growing to the same titre as wild-type rIBV at 48 h p.i. When administered to 1-day-old chickens, rIBV-Δ3a, rIBV-Δ3b, rIBV-Δ5a and rIBV-Δ5b showed reduced ciliostasis in comparison to the wild-type viruses. In conclusion, individual deletion of accessory genes in IBV H52 resulted in mutant viruses with an attenuated phenotype. Microbiology Society 2018-10 2018-08-01 /pmc/articles/PMC7079694/ /pubmed/30067172 http://dx.doi.org/10.1099/jgv.0.001130 Text en © 2018 The Authors https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License.
spellingShingle Research Article
Laconi, Andrea
van Beurden, Steven J.
Berends, Alinda J.
Krämer-Kühl, Annika
Jansen, Christine A.
Spekreijse, Dieuwertje
Chénard, Gilles
Philipp, Hans-Christian
Mundt, Egbert
Rottier, Peter J. M.
Hélène Verheije, M.
Deletion of accessory genes 3a, 3b, 5a or 5b from avian coronavirus infectious bronchitis virus induces an attenuated phenotype both in vitro and in vivo
title Deletion of accessory genes 3a, 3b, 5a or 5b from avian coronavirus infectious bronchitis virus induces an attenuated phenotype both in vitro and in vivo
title_full Deletion of accessory genes 3a, 3b, 5a or 5b from avian coronavirus infectious bronchitis virus induces an attenuated phenotype both in vitro and in vivo
title_fullStr Deletion of accessory genes 3a, 3b, 5a or 5b from avian coronavirus infectious bronchitis virus induces an attenuated phenotype both in vitro and in vivo
title_full_unstemmed Deletion of accessory genes 3a, 3b, 5a or 5b from avian coronavirus infectious bronchitis virus induces an attenuated phenotype both in vitro and in vivo
title_short Deletion of accessory genes 3a, 3b, 5a or 5b from avian coronavirus infectious bronchitis virus induces an attenuated phenotype both in vitro and in vivo
title_sort deletion of accessory genes 3a, 3b, 5a or 5b from avian coronavirus infectious bronchitis virus induces an attenuated phenotype both in vitro and in vivo
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7079694/
https://www.ncbi.nlm.nih.gov/pubmed/30067172
http://dx.doi.org/10.1099/jgv.0.001130
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