An in silico and in vitro pipeline for the rapid screening of helicase modulators

To evaluate the potency of potential helicase modulators, we developed an assay of helicase enzyme activity. Using a DNA or RNA biotin labelled oligonucleotide and after the addition of a recombinant helicase, the nucleic acid unwinds, causing the emission of luminescence, which is quantified with a...

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Detalles Bibliográficos
Autores principales: Papakonstantinou, Eleni, Bacopoulou, Flora, Megalooikonomou, Vasileios, Efthimiadou, Aspasia, Vlachakis, Dimitrios
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7079759/
https://www.ncbi.nlm.nih.gov/pubmed/32190583
http://dx.doi.org/10.14806/ej.25.0.927

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7079759/
https://www.ncbi.nlm.nih.gov/pubmed/32190583
http://dx.doi.org/10.14806/ej.25.0.927

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