Cargando…

Development of a monoclonal antibody-based enzyme-linked immunosorbent assay for the analysis of glycyrrhizic acid

Glycyrrhizic acid (GL) is a major active compound of licorice. The specific monoclonal antibody (MAb) (designated as 8F(8)A(8)H(4)2H(7)) against GL was produced with the immunogen GL–BSA conjugate. The dissociation constant (K (d)) value of the MAb was approximately 9.96×10(−10) M. The cross reactiv...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhao, Jing, Li, Gang, Wang, Bao-min, Liu, Wei, Nan, Tie-gui, Zhai, Zhi-xi, Li, Zhao-hu, Li, Qing X.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer-Verlag 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7079850/
https://www.ncbi.nlm.nih.gov/pubmed/17006677
http://dx.doi.org/10.1007/s00216-006-0780-z
_version_ 1783507904169508864
author Zhao, Jing
Li, Gang
Wang, Bao-min
Liu, Wei
Nan, Tie-gui
Zhai, Zhi-xi
Li, Zhao-hu
Li, Qing X.
author_facet Zhao, Jing
Li, Gang
Wang, Bao-min
Liu, Wei
Nan, Tie-gui
Zhai, Zhi-xi
Li, Zhao-hu
Li, Qing X.
author_sort Zhao, Jing
collection PubMed
description Glycyrrhizic acid (GL) is a major active compound of licorice. The specific monoclonal antibody (MAb) (designated as 8F(8)A(8)H(4)2H(7)) against GL was produced with the immunogen GL–BSA conjugate. The dissociation constant (K (d)) value of the MAb was approximately 9.96×10(−10) M. The cross reactivity of the MAb with glycyrrhetic acid was approximately 2.6%. The conventional indirect competitive enzyme-linked immunosorbent assay (icELISA) and simplified icELISA adapted with a modified procedure were established using the MAb. The IC(50) value and the detect range by the conventional icELISA were 1.1 ng mL(−1) and 0.2–5.1 ng mL(−1), respectively. The IC(50) value and the detect range by the simplified icELISA were 5.3 ng mL(−1) and 1.2–23.8 ng mL(−1), respectively. The two icELISA formats were used to analyze GL contents in the roots of wild licorice and different parts of cultivated licorice (Glycyrrhiza uralensis Fisch). The results obtained with the two icELISAs agreed well with those of the HPLC analysis. The correlation coefficient was more than 0.98 between HPLC and the two icELISAs. The two icELISAs were shown to be appropriate, simple, and effective for the quality control of raw licorice root materials.
format Online
Article
Text
id pubmed-7079850
institution National Center for Biotechnology Information
language English
publishDate 2006
publisher Springer-Verlag
record_format MEDLINE/PubMed
spelling pubmed-70798502020-03-23 Development of a monoclonal antibody-based enzyme-linked immunosorbent assay for the analysis of glycyrrhizic acid Zhao, Jing Li, Gang Wang, Bao-min Liu, Wei Nan, Tie-gui Zhai, Zhi-xi Li, Zhao-hu Li, Qing X. Anal Bioanal Chem Original Paper Glycyrrhizic acid (GL) is a major active compound of licorice. The specific monoclonal antibody (MAb) (designated as 8F(8)A(8)H(4)2H(7)) against GL was produced with the immunogen GL–BSA conjugate. The dissociation constant (K (d)) value of the MAb was approximately 9.96×10(−10) M. The cross reactivity of the MAb with glycyrrhetic acid was approximately 2.6%. The conventional indirect competitive enzyme-linked immunosorbent assay (icELISA) and simplified icELISA adapted with a modified procedure were established using the MAb. The IC(50) value and the detect range by the conventional icELISA were 1.1 ng mL(−1) and 0.2–5.1 ng mL(−1), respectively. The IC(50) value and the detect range by the simplified icELISA were 5.3 ng mL(−1) and 1.2–23.8 ng mL(−1), respectively. The two icELISA formats were used to analyze GL contents in the roots of wild licorice and different parts of cultivated licorice (Glycyrrhiza uralensis Fisch). The results obtained with the two icELISAs agreed well with those of the HPLC analysis. The correlation coefficient was more than 0.98 between HPLC and the two icELISAs. The two icELISAs were shown to be appropriate, simple, and effective for the quality control of raw licorice root materials. Springer-Verlag 2006-09-28 2006 /pmc/articles/PMC7079850/ /pubmed/17006677 http://dx.doi.org/10.1007/s00216-006-0780-z Text en © Springer-Verlag 2006 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Original Paper
Zhao, Jing
Li, Gang
Wang, Bao-min
Liu, Wei
Nan, Tie-gui
Zhai, Zhi-xi
Li, Zhao-hu
Li, Qing X.
Development of a monoclonal antibody-based enzyme-linked immunosorbent assay for the analysis of glycyrrhizic acid
title Development of a monoclonal antibody-based enzyme-linked immunosorbent assay for the analysis of glycyrrhizic acid
title_full Development of a monoclonal antibody-based enzyme-linked immunosorbent assay for the analysis of glycyrrhizic acid
title_fullStr Development of a monoclonal antibody-based enzyme-linked immunosorbent assay for the analysis of glycyrrhizic acid
title_full_unstemmed Development of a monoclonal antibody-based enzyme-linked immunosorbent assay for the analysis of glycyrrhizic acid
title_short Development of a monoclonal antibody-based enzyme-linked immunosorbent assay for the analysis of glycyrrhizic acid
title_sort development of a monoclonal antibody-based enzyme-linked immunosorbent assay for the analysis of glycyrrhizic acid
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7079850/
https://www.ncbi.nlm.nih.gov/pubmed/17006677
http://dx.doi.org/10.1007/s00216-006-0780-z
work_keys_str_mv AT zhaojing developmentofamonoclonalantibodybasedenzymelinkedimmunosorbentassayfortheanalysisofglycyrrhizicacid
AT ligang developmentofamonoclonalantibodybasedenzymelinkedimmunosorbentassayfortheanalysisofglycyrrhizicacid
AT wangbaomin developmentofamonoclonalantibodybasedenzymelinkedimmunosorbentassayfortheanalysisofglycyrrhizicacid
AT liuwei developmentofamonoclonalantibodybasedenzymelinkedimmunosorbentassayfortheanalysisofglycyrrhizicacid
AT nantiegui developmentofamonoclonalantibodybasedenzymelinkedimmunosorbentassayfortheanalysisofglycyrrhizicacid
AT zhaizhixi developmentofamonoclonalantibodybasedenzymelinkedimmunosorbentassayfortheanalysisofglycyrrhizicacid
AT lizhaohu developmentofamonoclonalantibodybasedenzymelinkedimmunosorbentassayfortheanalysisofglycyrrhizicacid
AT liqingx developmentofamonoclonalantibodybasedenzymelinkedimmunosorbentassayfortheanalysisofglycyrrhizicacid