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Current applications and future trends of molecular diagnostics in clinical bacteriology

Molecular diagnostics of infectious diseases, in particular, nucleic-acid-based methods, are the fastest growing field in clinical laboratory diagnostics. These applications are stepwise replacing or complementing culture-based, biochemical, and immunological assays in microbiology laboratories. The...

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Detalles Bibliográficos
Autores principales: Weile, Jan, Knabbe, Cornelius
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7079892/
https://www.ncbi.nlm.nih.gov/pubmed/19377839
http://dx.doi.org/10.1007/s00216-009-2779-8
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author Weile, Jan
Knabbe, Cornelius
author_facet Weile, Jan
Knabbe, Cornelius
author_sort Weile, Jan
collection PubMed
description Molecular diagnostics of infectious diseases, in particular, nucleic-acid-based methods, are the fastest growing field in clinical laboratory diagnostics. These applications are stepwise replacing or complementing culture-based, biochemical, and immunological assays in microbiology laboratories. The first-generation nucleic acid assays were monoparametric such as conventional tests, determining only a single parameter. Improvements and new approaches in technology now open the possibility for the development of multiparameter assays using microarrays, multiplex nucleic acid amplification techniques, or mass spectrometry, while the introduction of closed-tube systems has resulted in rapid microbial diagnostics with a subsequently reduced contamination risk. Whereas the first assays were focused on the detection and identification of microbial pathogens, these new technologies paved the way for the parallel determination of multiple antibiotic resistance determinants or to perform microbial epidemiology and surveillance on a genetic level.
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spelling pubmed-70798922020-03-23 Current applications and future trends of molecular diagnostics in clinical bacteriology Weile, Jan Knabbe, Cornelius Anal Bioanal Chem Review Molecular diagnostics of infectious diseases, in particular, nucleic-acid-based methods, are the fastest growing field in clinical laboratory diagnostics. These applications are stepwise replacing or complementing culture-based, biochemical, and immunological assays in microbiology laboratories. The first-generation nucleic acid assays were monoparametric such as conventional tests, determining only a single parameter. Improvements and new approaches in technology now open the possibility for the development of multiparameter assays using microarrays, multiplex nucleic acid amplification techniques, or mass spectrometry, while the introduction of closed-tube systems has resulted in rapid microbial diagnostics with a subsequently reduced contamination risk. Whereas the first assays were focused on the detection and identification of microbial pathogens, these new technologies paved the way for the parallel determination of multiple antibiotic resistance determinants or to perform microbial epidemiology and surveillance on a genetic level. Springer Berlin Heidelberg 2009-04-18 2009 /pmc/articles/PMC7079892/ /pubmed/19377839 http://dx.doi.org/10.1007/s00216-009-2779-8 Text en © Springer-Verlag 2009 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Review
Weile, Jan
Knabbe, Cornelius
Current applications and future trends of molecular diagnostics in clinical bacteriology
title Current applications and future trends of molecular diagnostics in clinical bacteriology
title_full Current applications and future trends of molecular diagnostics in clinical bacteriology
title_fullStr Current applications and future trends of molecular diagnostics in clinical bacteriology
title_full_unstemmed Current applications and future trends of molecular diagnostics in clinical bacteriology
title_short Current applications and future trends of molecular diagnostics in clinical bacteriology
title_sort current applications and future trends of molecular diagnostics in clinical bacteriology
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7079892/
https://www.ncbi.nlm.nih.gov/pubmed/19377839
http://dx.doi.org/10.1007/s00216-009-2779-8
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