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Soluble cytoplasmic expression, rapid purification, and characterization of cyanovirin-N as a His-SUMO fusion
Cyanovirin-N (CVN) is a promising antiviral candidate that has an extremely low sequence homology with any other known proteins. The efficient and soluble expression of biologically functional recombinant CVN (rCVN) is still an obstacle due to insufficient yield, aggregation, and abnormal modificati...
Autores principales: | Gao, Xianglei, Chen, Wei, Guo, Chaowan, Qian, Chuiwen, Liu, Ge, Ge, Feng, Huang, Yadong, Kitazato, Kaio, Wang, Yifei, Xiong, Sheng |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer-Verlag
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7080120/ https://www.ncbi.nlm.nih.gov/pubmed/19547966 http://dx.doi.org/10.1007/s00253-009-2078-5 |
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