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ST534: the new sequence type of Corynebacterium diphtheriae causing diphtheria in Jakarta and surrounding areas, Indonesia

BACKGROUND/AIM: The aim of this study was to find out characteristics and patterns of the spread of Corynebacterium diphtheriae isolated from Jakarta and the surrounding areas, using the whole genome sequencing (WGS) technique and multilocus sequence typing (MLST) approach. MATERIALS AND METHODS: Th...

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Autores principales: SUNARNO, Sunarno, RUKMINIATI, Yuni, SARASWATI, Ratih Dian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Scientific and Technological Research Council of Turkey 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7080344/
https://www.ncbi.nlm.nih.gov/pubmed/31769641
http://dx.doi.org/10.3906/sag-1909-4
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author SUNARNO, Sunarno
RUKMINIATI, Yuni
SARASWATI, Ratih Dian
author_facet SUNARNO, Sunarno
RUKMINIATI, Yuni
SARASWATI, Ratih Dian
author_sort SUNARNO, Sunarno
collection PubMed
description BACKGROUND/AIM: The aim of this study was to find out characteristics and patterns of the spread of Corynebacterium diphtheriae isolated from Jakarta and the surrounding areas, using the whole genome sequencing (WGS) technique and multilocus sequence typing (MLST) approach. MATERIALS AND METHODS: The study samples consisted of 86 C. diphtheriae isolates, which were isolated from diphtheria patients and close contacts of patients. The DNA sequencing was carried out using the WGS technique. Data conversion applied the U-gene software. Molecular typing was conducted through the MLST approach, then followed by online data analysis. RESULTS: The results showed that as many as 43 (50%) of all samples examined were new types with the same allele profile, namely 9-1-13-4-3-3-4. New sequence type C. diphtheriae is registered in the MLST global database as ST534 based on the allele profile. The tox gene analysis in 43 isolates with ST534 indicated that there were three mutation positions, all of which were silent mutations. CONCLUSION: The main cause of diphtheria in Jakarta and the surrounding areas is a new sequence type of C. diphtheriae registered as ST534.
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spelling pubmed-70803442020-03-23 ST534: the new sequence type of Corynebacterium diphtheriae causing diphtheria in Jakarta and surrounding areas, Indonesia SUNARNO, Sunarno RUKMINIATI, Yuni SARASWATI, Ratih Dian Turk J Med Sci Article BACKGROUND/AIM: The aim of this study was to find out characteristics and patterns of the spread of Corynebacterium diphtheriae isolated from Jakarta and the surrounding areas, using the whole genome sequencing (WGS) technique and multilocus sequence typing (MLST) approach. MATERIALS AND METHODS: The study samples consisted of 86 C. diphtheriae isolates, which were isolated from diphtheria patients and close contacts of patients. The DNA sequencing was carried out using the WGS technique. Data conversion applied the U-gene software. Molecular typing was conducted through the MLST approach, then followed by online data analysis. RESULTS: The results showed that as many as 43 (50%) of all samples examined were new types with the same allele profile, namely 9-1-13-4-3-3-4. New sequence type C. diphtheriae is registered in the MLST global database as ST534 based on the allele profile. The tox gene analysis in 43 isolates with ST534 indicated that there were three mutation positions, all of which were silent mutations. CONCLUSION: The main cause of diphtheria in Jakarta and the surrounding areas is a new sequence type of C. diphtheriae registered as ST534. The Scientific and Technological Research Council of Turkey 2020-02-13 /pmc/articles/PMC7080344/ /pubmed/31769641 http://dx.doi.org/10.3906/sag-1909-4 Text en Copyright © 2019 The Author(s) This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Article
SUNARNO, Sunarno
RUKMINIATI, Yuni
SARASWATI, Ratih Dian
ST534: the new sequence type of Corynebacterium diphtheriae causing diphtheria in Jakarta and surrounding areas, Indonesia
title ST534: the new sequence type of Corynebacterium diphtheriae causing diphtheria in Jakarta and surrounding areas, Indonesia
title_full ST534: the new sequence type of Corynebacterium diphtheriae causing diphtheria in Jakarta and surrounding areas, Indonesia
title_fullStr ST534: the new sequence type of Corynebacterium diphtheriae causing diphtheria in Jakarta and surrounding areas, Indonesia
title_full_unstemmed ST534: the new sequence type of Corynebacterium diphtheriae causing diphtheria in Jakarta and surrounding areas, Indonesia
title_short ST534: the new sequence type of Corynebacterium diphtheriae causing diphtheria in Jakarta and surrounding areas, Indonesia
title_sort st534: the new sequence type of corynebacterium diphtheriae causing diphtheria in jakarta and surrounding areas, indonesia
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7080344/
https://www.ncbi.nlm.nih.gov/pubmed/31769641
http://dx.doi.org/10.3906/sag-1909-4
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