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4D Self‐Morphing Culture Substrate for Modulating Cell Differentiation

As the most versatile and promising cell source, stem cells have been studied in regenerative medicine for two decades. Currently available culturing techniques utilize a 2D or 3D microenvironment for supporting the growth and proliferation of stem cells. However, these culture systems fail to fully...

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Autores principales: Miao, Shida, Cui, Haitao, Esworthy, Timothy, Mahadik, Bhushan, Lee, Se‐jun, Zhou, Xuan, Hann, Sung Yun, Fisher, John P., Zhang, Lijie Grace
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7080541/
https://www.ncbi.nlm.nih.gov/pubmed/32195081
http://dx.doi.org/10.1002/advs.201902403
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author Miao, Shida
Cui, Haitao
Esworthy, Timothy
Mahadik, Bhushan
Lee, Se‐jun
Zhou, Xuan
Hann, Sung Yun
Fisher, John P.
Zhang, Lijie Grace
author_facet Miao, Shida
Cui, Haitao
Esworthy, Timothy
Mahadik, Bhushan
Lee, Se‐jun
Zhou, Xuan
Hann, Sung Yun
Fisher, John P.
Zhang, Lijie Grace
author_sort Miao, Shida
collection PubMed
description As the most versatile and promising cell source, stem cells have been studied in regenerative medicine for two decades. Currently available culturing techniques utilize a 2D or 3D microenvironment for supporting the growth and proliferation of stem cells. However, these culture systems fail to fully reflect the supportive biological environment in which stem cells reside in vivo, which contain dynamic biophysical growth cues. Herein, a 4D programmable culture substrate with a self‐morphing capability is presented as a means to enhance dynamic cell growth and induce differentiation of stem cells. To function as a model system, a 4D neural culture substrate is fabricated using a combination of printing and imprinting techniques keyed to the different biological features of neural stem cells (NSCs) at different differentiation stages. Results show the 4D culture substrate demonstrates a time‐dependent self‐morphing process that plays an essential role in regulating NSC behaviors in a spatiotemporal manner and enhances neural differentiation of NSCs along with significant axonal alignment. This study of a customized, dynamic substrate revolutionizes current stem cell therapies, and can further have a far‐reaching impact on improving tissue regeneration and mimicking specific disease progression, as well as other impacts on materials and life science research.
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spelling pubmed-70805412020-03-19 4D Self‐Morphing Culture Substrate for Modulating Cell Differentiation Miao, Shida Cui, Haitao Esworthy, Timothy Mahadik, Bhushan Lee, Se‐jun Zhou, Xuan Hann, Sung Yun Fisher, John P. Zhang, Lijie Grace Adv Sci (Weinh) Full Papers As the most versatile and promising cell source, stem cells have been studied in regenerative medicine for two decades. Currently available culturing techniques utilize a 2D or 3D microenvironment for supporting the growth and proliferation of stem cells. However, these culture systems fail to fully reflect the supportive biological environment in which stem cells reside in vivo, which contain dynamic biophysical growth cues. Herein, a 4D programmable culture substrate with a self‐morphing capability is presented as a means to enhance dynamic cell growth and induce differentiation of stem cells. To function as a model system, a 4D neural culture substrate is fabricated using a combination of printing and imprinting techniques keyed to the different biological features of neural stem cells (NSCs) at different differentiation stages. Results show the 4D culture substrate demonstrates a time‐dependent self‐morphing process that plays an essential role in regulating NSC behaviors in a spatiotemporal manner and enhances neural differentiation of NSCs along with significant axonal alignment. This study of a customized, dynamic substrate revolutionizes current stem cell therapies, and can further have a far‐reaching impact on improving tissue regeneration and mimicking specific disease progression, as well as other impacts on materials and life science research. John Wiley and Sons Inc. 2020-02-18 /pmc/articles/PMC7080541/ /pubmed/32195081 http://dx.doi.org/10.1002/advs.201902403 Text en © 2020 The Authors. Published by WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Full Papers
Miao, Shida
Cui, Haitao
Esworthy, Timothy
Mahadik, Bhushan
Lee, Se‐jun
Zhou, Xuan
Hann, Sung Yun
Fisher, John P.
Zhang, Lijie Grace
4D Self‐Morphing Culture Substrate for Modulating Cell Differentiation
title 4D Self‐Morphing Culture Substrate for Modulating Cell Differentiation
title_full 4D Self‐Morphing Culture Substrate for Modulating Cell Differentiation
title_fullStr 4D Self‐Morphing Culture Substrate for Modulating Cell Differentiation
title_full_unstemmed 4D Self‐Morphing Culture Substrate for Modulating Cell Differentiation
title_short 4D Self‐Morphing Culture Substrate for Modulating Cell Differentiation
title_sort 4d self‐morphing culture substrate for modulating cell differentiation
topic Full Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7080541/
https://www.ncbi.nlm.nih.gov/pubmed/32195081
http://dx.doi.org/10.1002/advs.201902403
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