Cargando…

Build-up functionalization of anti-EGFR × anti-CD3 bispecific diabodies by integrating high-affinity mutants and functional molecular formats

Designing non-natural antibody formats is a practical method for developing highly functional next-generation antibody drugs, particularly for improving the therapeutic efficacy of cancer treatments. One approach is constructing bispecific antibodies (bsAbs). We previously reported a functional huma...

Descripción completa

Detalles Bibliográficos
Autores principales:	Asano, Ryutaro, Hosokawa, Katsuhiro, Taki, Shintaro, Konno, Shota, Shimomura, Ippei, Ogata, Hiromi, Okada, Mai, Arai, Kyoko, Onitsuka, Masayoshi, Omasa, Takeshi, Nakanishi, Takeshi, Umetsu, Mitsuo, Kumagai, Izumi
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2020
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7080790/ https://www.ncbi.nlm.nih.gov/pubmed/32188928 http://dx.doi.org/10.1038/s41598-020-61840-3

Ejemplares similares

Rearranging the domain order of a diabody-based IgG-like bispecific antibody enhances its antitumor activity and improves its degradation resistance and pharmacokinetics
por: Asano, Ryutaro, et al.
Publicado: (2014)

Structural considerations for functional anti-EGFR × anti-CD3 bispecific diabodies in light of domain order and binding affinity
por: Asano, Ryutaro, et al.
Publicado: (2018)

Functional Domain Order of an Anti-EGFR × Anti-CD16 Bispecific Diabody Involving NK Cell Activation
por: Kuwahara, Atsushi, et al.
Publicado: (2020)

A semi high-throughput method for screening small bispecific antibodies with high cytotoxicity
por: Sugiyama, Aruto, et al.
Publicado: (2017)

Chemically Crosslinked Bispecific Antibodies for Cancer Therapy: Breaking from the Structural Restrictions of the Genetic Fusion Approach
por: Ueda, Asami, et al.
Publicado: (2020)

Affinity maturation of humanized anti-epidermal growth factor receptor antibody using a modified phage-based open sandwich selection method
por: Sanada, Hideaki, et al.
Publicado: (2018)

Development of bispecific anti-c-Met/PD-1 diabodies for the treatment of solid tumors and the effect of c-Met binding affinity on efficacy
por: Yuan, Qingyun, et al.
Publicado: (2021)

Anti‐EGFR scFv tetramer (tetrabody) with a stable monodisperse structure, strong anticancer effect, and a long in vivo half‐life
por: Asano, Ryutaro, et al.
Publicado: (2016)

Balanced secretion of anti-CEA × anti-CD3 diabody chains using the 2A self-cleaving peptide maximizes diabody assembly and tumor-specific cytotoxicity
por: Mølgaard, K, et al.
Publicado: (2017)

Engineering the hinge region of human IgG1 Fc-fused bispecific antibodies to improve fragmentation resistance
por: Suzuki, Saori, et al.
Publicado: (2018)

Chemical chaperone suppresses the antibody aggregation in CHO cell culture
por: Onitsuka, Masayoshi, et al.
Publicado: (2013)

Increased antibody productivity in Chinese Hamster Ovary cells through induction of chromosomal instability by cell fusion
por: Lai, YuanShan, et al.
Publicado: (2015)

Construction of IgG–Fab(2) bispecific antibody via intein-mediated protein trans-splicing reaction
por: Yamada, Risa, et al.
Publicado: (2023)

Dynamical analysis of antibody aggregation in the CHO cell culture with Thermo Responsive Protein A (TRPA) column
por: Noda, Masahiro, et al.
Publicado: (2013)

Construction of a gene knockout CHO cell line using a simple gene targeting method
por: Aga, Marina, et al.
Publicado: (2015)

Stability difference of each chromosome in Chinese Hamster Ovary cell line
por: Yamano, Noriko, et al.
Publicado: (2015)

DeBouganin Diabody Fusion Protein Overcomes Drug Resistance to ADCs Comprised of Anti-Microtubule Agents
por: Chooniedass, Shilpa, et al.
Publicado: (2016)

A bispecific diabody directed against prostate-specific membrane antigen and CD3 induces T-cell mediated lysis of prostate cancer cells
por: Bühler, P., et al.
Publicado: (2007)

T Cell Bispecific Antibodies: An Antibody-Based Delivery System for Inducing Antitumor Immunity
por: Kamakura, Daisuke, et al.
Publicado: (2021)

Structural and functional characterization of MBS301, an afucosylated bispecific anti-HER2 antibody
por: Huang, Sijia, et al.
Publicado: (2018)

Generation and Characterization of a Diabody Targeting the α(v)β(6) Integrin
por: Kogelberg, Heide, et al.
Publicado: (2013)

Anti-EGFR antibody 528 binds to domain III of EGFR at a site shifted from the cetuximab epitope
por: Makabe, Koki, et al.
Publicado: (2021)

Monoclonal and Bispecific Anti-BCMA Antibodies in Multiple Myeloma
por: Dalla Palma, Benedetta, et al.
Publicado: (2020)

Optimized Expression and Characterization of a Novel Fully Human Bispecific Single-Chain Diabody Targeting Vascular Endothelial Growth Factor165 and Programmed Death-1 in Pichia pastoris and Evaluation of Antitumor Activity In Vivo
por: Xiong, Chenghao, et al.
Publicado: (2018)

Improvement of the Targeting of Radiolabeled and Functionalized Liposomes with a Two-Step System Using a Bispecific Monoclonal Antibody (Anti-CEA × Anti-DTPA–In)
por: Rauscher, Aurore, et al.
Publicado: (2015)

Mechanism of action of a T cell-dependent bispecific antibody as a breakthrough immunotherapy against refractory colorectal cancer with an oncogenic mutation
por: Kamakura, Daisuke, et al.
Publicado: (2020)

Inhibition activity of a disulfide-stabilized diabody against basic fibroblast growth factor in lung cancer
por: Cai, Yaxiong, et al.
Publicado: (2017)

Fermentation, Purification, and Tumor Inhibition of a Disulfide-Stabilized Diabody Against Fibroblast Growth Factor-2
por: Zhang, Simin, et al.
Publicado: (2021)

Cancer therapeutic trispecific antibodies recruiting both T and natural killer cells to cancer cells
por: Kimura, Kouki, et al.
Publicado: (2023)

Atorvastatin Inhibits the HIF1α-PPAR Axis, Which Is Essential for Maintaining the Function of Human Induced Pluripotent Stem Cells
por: Nakashima, Yoshiki, et al.
Publicado: (2018)

Antitumor Activity of an Anti-EGFR/HER2 Bispecific Antibody in a Mouse Xenograft Model of Canine Osteosarcoma
por: Tateyama, Nami, et al.
Publicado: (2022)

Functional comparison of single-chain and two-chain anti-CD3-based bispecific antibodies in gene immunotherapy applications
por: Compte, Marta, et al.
Publicado: (2014)

Analyses of functions of an anti-PD-L1/TGFβR2 bispecific fusion protein (M7824)
por: Jochems, Caroline, et al.
Publicado: (2017)

Prolonged in vivo tumour retention of a human diabody targeting the extracellular domain of human HER2/neu.
por: Adams, G. P., et al.
Publicado: (1998)

Crystal structures of mono- and bi-specific diabodies and reduction of their structural flexibility by introduction of disulfide bridges at the Fv interface
por: Kim, Jin Hong, et al.
Publicado: (2016)

Distinguishing Between Monomeric scFv and Diabody in Solution Using Light and Small Angle X-ray Scattering
por: Lüdel, Frank, et al.
Publicado: (2019)

Anti-influenza A virus effects of fructan from Welsh onion (Allium fistulosum L.)
por: Lee, Jung-Bum, et al.
Publicado: (2012)

Positron emission tomography imaging with (89)Zr-labeled anti-CD8 cys-diabody reveals CD8(+) cell infiltration during oncolytic virus therapy in a glioma murine model
por: Kasten, Benjamin B., et al.
Publicado: (2021)

What Kind of Signaling Maintains Pluripotency and Viability in Human-Induced Pluripotent Stem Cells Cultured on Laminin-511 with Serum-Free Medium?
por: Nakashima, Yoshiki, et al.
Publicado: (2016)

In vitro and in vivo stability and anti-tumour efficacy of an anti-EGFR/anti-CD3 F(ab')2 bispecific monoclonal antibody.
por: Negri, D. R., et al.
Publicado: (1995)

Cannot write session to /tmp/vufind_sessions/sess_epsftc6ves5h3m8fcbe8i9k74n