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A simple method for short-term maintenance of neonatal mice without foster mothers

Mice are typically weaned from their mother between 21 and 28 days of age, or at 10 grams of body weight. However, some biochemical experiments need to be done before the weaning days, and the mother might cannibalize or ignore those manipulated pups. Here, we provide a detailed protocol for mainten...

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Detalles Bibliográficos
Autores principales: Kreikemeier-Bower, Craig, Polepole, Pascal, Pinkerton, Katherine, Zhang, Luwen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Journal of Biological Methods 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7081055/
https://www.ncbi.nlm.nih.gov/pubmed/32201708
http://dx.doi.org/10.14440/jbm.2020.315
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author Kreikemeier-Bower, Craig
Polepole, Pascal
Pinkerton, Katherine
Zhang, Luwen
author_facet Kreikemeier-Bower, Craig
Polepole, Pascal
Pinkerton, Katherine
Zhang, Luwen
author_sort Kreikemeier-Bower, Craig
collection PubMed
description Mice are typically weaned from their mother between 21 and 28 days of age, or at 10 grams of body weight. However, some biochemical experiments need to be done before the weaning days, and the mother might cannibalize or ignore those manipulated pups. Here, we provide a detailed protocol for maintenance of neonatal mice without the presence of their mothers for biomedical research. The basic instinct of neonate mice to hide under covers is harnessed for their survival in a mother-free environment. When covers are soaked with milk and the only targets for hiding, the neonates would acquire their nutrients at least in an involuntary fashion. The protocol is simple and can be used for neonatal rodent studies for short periods of times, and assures the accuracy of the biomedical experiments if survival rate of neonates is critical.
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spelling pubmed-70810552020-03-20 A simple method for short-term maintenance of neonatal mice without foster mothers Kreikemeier-Bower, Craig Polepole, Pascal Pinkerton, Katherine Zhang, Luwen J Biol Methods Protocol Mice are typically weaned from their mother between 21 and 28 days of age, or at 10 grams of body weight. However, some biochemical experiments need to be done before the weaning days, and the mother might cannibalize or ignore those manipulated pups. Here, we provide a detailed protocol for maintenance of neonatal mice without the presence of their mothers for biomedical research. The basic instinct of neonate mice to hide under covers is harnessed for their survival in a mother-free environment. When covers are soaked with milk and the only targets for hiding, the neonates would acquire their nutrients at least in an involuntary fashion. The protocol is simple and can be used for neonatal rodent studies for short periods of times, and assures the accuracy of the biomedical experiments if survival rate of neonates is critical. Journal of Biological Methods 2020-02-17 /pmc/articles/PMC7081055/ /pubmed/32201708 http://dx.doi.org/10.14440/jbm.2020.315 Text en © 2013-2020 The Journal of Biological Methods, All rights reserved. http://creativecommons.org/licenses/by-nc-sa/4.0 This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License: http://creativecommons.org/licenses/by-nc-sa/4.0
spellingShingle Protocol
Kreikemeier-Bower, Craig
Polepole, Pascal
Pinkerton, Katherine
Zhang, Luwen
A simple method for short-term maintenance of neonatal mice without foster mothers
title A simple method for short-term maintenance of neonatal mice without foster mothers
title_full A simple method for short-term maintenance of neonatal mice without foster mothers
title_fullStr A simple method for short-term maintenance of neonatal mice without foster mothers
title_full_unstemmed A simple method for short-term maintenance of neonatal mice without foster mothers
title_short A simple method for short-term maintenance of neonatal mice without foster mothers
title_sort simple method for short-term maintenance of neonatal mice without foster mothers
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7081055/
https://www.ncbi.nlm.nih.gov/pubmed/32201708
http://dx.doi.org/10.14440/jbm.2020.315
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