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Blood meal sources and bacterial microbiome diversity in wild-caught tsetse flies

Tsetse flies are the vectors of African trypanosomiasis affecting 36 sub-Saharan countries. Both wild and domestic animals play a crucial role in maintaining the disease-causing parasites (trypanosomes). Thus, the identification of animal reservoirs of trypanosomes is vital for the effective control...

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Autores principales: Gaithuma, Alex, Yamagishi, Junya, Hayashida, Kyoko, Kawai, Naoko, Namangala, Boniface, Sugimoto, Chihiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7081217/
https://www.ncbi.nlm.nih.gov/pubmed/32193415
http://dx.doi.org/10.1038/s41598-020-61817-2
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author Gaithuma, Alex
Yamagishi, Junya
Hayashida, Kyoko
Kawai, Naoko
Namangala, Boniface
Sugimoto, Chihiro
author_facet Gaithuma, Alex
Yamagishi, Junya
Hayashida, Kyoko
Kawai, Naoko
Namangala, Boniface
Sugimoto, Chihiro
author_sort Gaithuma, Alex
collection PubMed
description Tsetse flies are the vectors of African trypanosomiasis affecting 36 sub-Saharan countries. Both wild and domestic animals play a crucial role in maintaining the disease-causing parasites (trypanosomes). Thus, the identification of animal reservoirs of trypanosomes is vital for the effective control of African trypanosomiasis. Additionally, the biotic and abiotic factors that drive gut microbiome diversity in tsetse flies are primarily unresolved, especially under natural, field conditions. In this study, we present a comprehensive DNA metabarcoding approach for individual tsetse fly analysis in the identification of mammalian blood meal sources and fly bacterial microbiome composition. We analyzed samples from two endemic foci, Kafue, Zambia collected in June 2017, and Hurungwe, Zimbabwe sampled in April 2014 (pilot study) and detected DNA of various mammals including humans, wild animals, domestic animals and small mammals (rat and bat). The bacterial diversity was relatively similar in flies with different mammalian species DNA, trypanosome infected and uninfected flies, and female and male flies. This study is the first report on bat DNA detection in wild tsetse flies. This study reveals that small mammals such as bats and rats are among the opportunistic blood meal sources for tsetse flies in the wild, and the implication on tsetse biology and ecology needs to be studied.
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spelling pubmed-70812172020-03-23 Blood meal sources and bacterial microbiome diversity in wild-caught tsetse flies Gaithuma, Alex Yamagishi, Junya Hayashida, Kyoko Kawai, Naoko Namangala, Boniface Sugimoto, Chihiro Sci Rep Article Tsetse flies are the vectors of African trypanosomiasis affecting 36 sub-Saharan countries. Both wild and domestic animals play a crucial role in maintaining the disease-causing parasites (trypanosomes). Thus, the identification of animal reservoirs of trypanosomes is vital for the effective control of African trypanosomiasis. Additionally, the biotic and abiotic factors that drive gut microbiome diversity in tsetse flies are primarily unresolved, especially under natural, field conditions. In this study, we present a comprehensive DNA metabarcoding approach for individual tsetse fly analysis in the identification of mammalian blood meal sources and fly bacterial microbiome composition. We analyzed samples from two endemic foci, Kafue, Zambia collected in June 2017, and Hurungwe, Zimbabwe sampled in April 2014 (pilot study) and detected DNA of various mammals including humans, wild animals, domestic animals and small mammals (rat and bat). The bacterial diversity was relatively similar in flies with different mammalian species DNA, trypanosome infected and uninfected flies, and female and male flies. This study is the first report on bat DNA detection in wild tsetse flies. This study reveals that small mammals such as bats and rats are among the opportunistic blood meal sources for tsetse flies in the wild, and the implication on tsetse biology and ecology needs to be studied. Nature Publishing Group UK 2020-03-19 /pmc/articles/PMC7081217/ /pubmed/32193415 http://dx.doi.org/10.1038/s41598-020-61817-2 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Gaithuma, Alex
Yamagishi, Junya
Hayashida, Kyoko
Kawai, Naoko
Namangala, Boniface
Sugimoto, Chihiro
Blood meal sources and bacterial microbiome diversity in wild-caught tsetse flies
title Blood meal sources and bacterial microbiome diversity in wild-caught tsetse flies
title_full Blood meal sources and bacterial microbiome diversity in wild-caught tsetse flies
title_fullStr Blood meal sources and bacterial microbiome diversity in wild-caught tsetse flies
title_full_unstemmed Blood meal sources and bacterial microbiome diversity in wild-caught tsetse flies
title_short Blood meal sources and bacterial microbiome diversity in wild-caught tsetse flies
title_sort blood meal sources and bacterial microbiome diversity in wild-caught tsetse flies
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7081217/
https://www.ncbi.nlm.nih.gov/pubmed/32193415
http://dx.doi.org/10.1038/s41598-020-61817-2
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