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Validation and Evaluation of High-Resolution Orbitrap Mass Spectrometry on Molecular Characterization of Dissolved Organic Matter
[Image: see text] Molecular composition of dissolved organic matter (DOM) is a hot topic in subjects such as environmental science and geochemistry. Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) has been applied to molecular composition characterization of DOM successfully....
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7081437/ https://www.ncbi.nlm.nih.gov/pubmed/32201827 http://dx.doi.org/10.1021/acsomega.9b04411 |
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author | Pan, Qiong Zhuo, Xiaocun He, Chen Zhang, Yahe Shi, Quan |
author_facet | Pan, Qiong Zhuo, Xiaocun He, Chen Zhang, Yahe Shi, Quan |
author_sort | Pan, Qiong |
collection | PubMed |
description | [Image: see text] Molecular composition of dissolved organic matter (DOM) is a hot topic in subjects such as environmental science and geochemistry. Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) has been applied to molecular composition characterization of DOM successfully. However, high instrument and maintenance costs have constrained its wider application. A high-resolution Orbitrap mass spectrometer (Orbitrap MS) can provide approximately 500,000 resolving power (at m/z 200), which is potentially capable of characterizing the molecular composition of DOM. In this paper, the application of high-resolution Orbitrap MS was evaluated by comparing with FT-ICR MS in the aspect of resolution, mass distribution, detection dynamic range, and isotopic peak intensity ratio. The impact of instrument parameters of Orbitrap MS was further investigated, which includes ionization, ion transfer, and mass detection. The result shows that the high-resolution Orbitrap MS is capable or even preferable for molecular characterization of DOM. However, the peak intensity distributions are dependent on the instrument parameters, which could affect the environmental impact assessment caused by the sample itself. The result indicates that development of a universal and comparable method is of great demand. |
format | Online Article Text |
id | pubmed-7081437 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-70814372020-03-20 Validation and Evaluation of High-Resolution Orbitrap Mass Spectrometry on Molecular Characterization of Dissolved Organic Matter Pan, Qiong Zhuo, Xiaocun He, Chen Zhang, Yahe Shi, Quan ACS Omega [Image: see text] Molecular composition of dissolved organic matter (DOM) is a hot topic in subjects such as environmental science and geochemistry. Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) has been applied to molecular composition characterization of DOM successfully. However, high instrument and maintenance costs have constrained its wider application. A high-resolution Orbitrap mass spectrometer (Orbitrap MS) can provide approximately 500,000 resolving power (at m/z 200), which is potentially capable of characterizing the molecular composition of DOM. In this paper, the application of high-resolution Orbitrap MS was evaluated by comparing with FT-ICR MS in the aspect of resolution, mass distribution, detection dynamic range, and isotopic peak intensity ratio. The impact of instrument parameters of Orbitrap MS was further investigated, which includes ionization, ion transfer, and mass detection. The result shows that the high-resolution Orbitrap MS is capable or even preferable for molecular characterization of DOM. However, the peak intensity distributions are dependent on the instrument parameters, which could affect the environmental impact assessment caused by the sample itself. The result indicates that development of a universal and comparable method is of great demand. American Chemical Society 2020-03-09 /pmc/articles/PMC7081437/ /pubmed/32201827 http://dx.doi.org/10.1021/acsomega.9b04411 Text en Copyright © 2020 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes. |
spellingShingle | Pan, Qiong Zhuo, Xiaocun He, Chen Zhang, Yahe Shi, Quan Validation and Evaluation of High-Resolution Orbitrap Mass Spectrometry on Molecular Characterization of Dissolved Organic Matter |
title | Validation and Evaluation of High-Resolution Orbitrap
Mass Spectrometry on Molecular Characterization of Dissolved Organic
Matter |
title_full | Validation and Evaluation of High-Resolution Orbitrap
Mass Spectrometry on Molecular Characterization of Dissolved Organic
Matter |
title_fullStr | Validation and Evaluation of High-Resolution Orbitrap
Mass Spectrometry on Molecular Characterization of Dissolved Organic
Matter |
title_full_unstemmed | Validation and Evaluation of High-Resolution Orbitrap
Mass Spectrometry on Molecular Characterization of Dissolved Organic
Matter |
title_short | Validation and Evaluation of High-Resolution Orbitrap
Mass Spectrometry on Molecular Characterization of Dissolved Organic
Matter |
title_sort | validation and evaluation of high-resolution orbitrap
mass spectrometry on molecular characterization of dissolved organic
matter |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7081437/ https://www.ncbi.nlm.nih.gov/pubmed/32201827 http://dx.doi.org/10.1021/acsomega.9b04411 |
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