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Optimization of small RNA library preparation protocol from human urinary exosomes

BACKGROUND: Sequencing of miRNAs isolated from exosomes has great potential to identify novel disease biomarkers, but exosomes have low amount of RNA, hindering adequate analysis and quantification. Here, we have assessed several steps in developing an optimized small RNA (sRNA) library preparation...

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Detalles Bibliográficos
Autores principales:	Olivares, Dolores, Perez-Hernandez, Javier, Perez-Gil, Daniel, Chaves, Felipe J., Redon, Josep, Cortes, Raquel
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2020
Materias:	Methodology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7081560/ https://www.ncbi.nlm.nih.gov/pubmed/32188466 http://dx.doi.org/10.1186/s12967-020-02298-9

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