Cargando…
Glutathione S-transferase M1 and T1 genes deletion polymorphisms and risk of developing essential hypertension: a case-control study in Burkina Faso population (West Africa)
BACKGROUND: Glutathione S-transferases play a key role in the detoxification of persistent oxidative stress products which are one of several risks factors that may be associated with many types of disease processes such as cancer, diabetes, and hypertension. In the present study, we characterize th...
Autores principales: | , , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7081581/ https://www.ncbi.nlm.nih.gov/pubmed/32188413 http://dx.doi.org/10.1186/s12881-020-0990-9 |
_version_ | 1783508198179733504 |
---|---|
author | Sombié, Herman Karim Sorgho, Abel Pegdwendé Kologo, Jonas Koudougou Ouattara, Abdoul Karim Yaméogo, Sakinata Yonli, Albert Théophane Djigma, Florencia Wendkuuni Tchelougou, Daméhan Somda, Dogfounianalo Kiendrébéogo, Isabelle Touwendpoulimdé Bado, Prosper Nagalo, Bolni Marius Nagabila, Youssoufou Adoko, Enagnon Tiémoko Herman Donald Zabsonré, Patrice Millogo, Hassanata Simporé, Jacques |
author_facet | Sombié, Herman Karim Sorgho, Abel Pegdwendé Kologo, Jonas Koudougou Ouattara, Abdoul Karim Yaméogo, Sakinata Yonli, Albert Théophane Djigma, Florencia Wendkuuni Tchelougou, Daméhan Somda, Dogfounianalo Kiendrébéogo, Isabelle Touwendpoulimdé Bado, Prosper Nagalo, Bolni Marius Nagabila, Youssoufou Adoko, Enagnon Tiémoko Herman Donald Zabsonré, Patrice Millogo, Hassanata Simporé, Jacques |
author_sort | Sombié, Herman Karim |
collection | PubMed |
description | BACKGROUND: Glutathione S-transferases play a key role in the detoxification of persistent oxidative stress products which are one of several risks factors that may be associated with many types of disease processes such as cancer, diabetes, and hypertension. In the present study, we characterize the null genotypes of GSTM1 and GSTT1 in order to investigate the association between them and the risk of developing essential hypertension. METHODS: We conducted a case-control study in Burkina Faso, including 245 subjects with essential hypertension as case and 269 control subjects with normal blood pressure. Presence of the GSTT1 and GSTM1 was determined using conventional multiplex polymerase chain reaction followed by gel electrophoresis analysis. Biochemical parameters were measured using chemistry analyzer CYANExpert 130. RESULTS: Chi-squared test shows that GSTT1-null (OR = 1.82; p = 0.001) and GSTM1-active/GSTT1-null genotypes (OR = 2.33; p < 0.001) were significantly higher in cases than controls; the differences were not significant for GSTM1-null, GSTM1-null/GSTT1-active and GSTM1-null/GSTT1-null (p > 0.05). Multinomial logistic regression revealed that age ≥ 50 years, central obesity, family history of hypertension, obesity, alcohol intake and GSTT1 deletion were in decreasing order independent risk factors for essential hypertension. Analysis by gender, BMI and alcohol showed that association of GSTT1-null with risk of essential hypertension seems to be significant when BMI < 30 Kg/m(2), in non-smokers and in alcohol users (all OR ≥ 1.77; p ≤ 0.008). Concerning GSTT1, GSTM1 and cardiovascular risk markers levels in hypertensive group, we found that subjects with GSTT1-null genotype had higher waist circumference and higher HDL cholesterol level than those with GSTT1-active (all p < 0.005), subjects with GSTM1-null genotype had lower triglyceride than those with GSTM1-active (p = 0.02) and subjects with the double deletion GSTM1-null/GSTT1-null had higher body mass index, higher waist circumference and higher HDL cholesterol than those with GSTM1-active/GSTT1-active genotype (all p = 0.01). CONCLUSION: Our results confirm that GSTT1-null genotype is significantly associated with risk of developing essential hypertension in Burkinabe, especially when BMI < 30 Kg/m(2), in non-smokers and in alcohol users, and it showed that the double deletion GSTM1-null/GSTT1-null genotypes may influence body lipids repartition. |
format | Online Article Text |
id | pubmed-7081581 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-70815812020-03-23 Glutathione S-transferase M1 and T1 genes deletion polymorphisms and risk of developing essential hypertension: a case-control study in Burkina Faso population (West Africa) Sombié, Herman Karim Sorgho, Abel Pegdwendé Kologo, Jonas Koudougou Ouattara, Abdoul Karim Yaméogo, Sakinata Yonli, Albert Théophane Djigma, Florencia Wendkuuni Tchelougou, Daméhan Somda, Dogfounianalo Kiendrébéogo, Isabelle Touwendpoulimdé Bado, Prosper Nagalo, Bolni Marius Nagabila, Youssoufou Adoko, Enagnon Tiémoko Herman Donald Zabsonré, Patrice Millogo, Hassanata Simporé, Jacques BMC Med Genet Research Article BACKGROUND: Glutathione S-transferases play a key role in the detoxification of persistent oxidative stress products which are one of several risks factors that may be associated with many types of disease processes such as cancer, diabetes, and hypertension. In the present study, we characterize the null genotypes of GSTM1 and GSTT1 in order to investigate the association between them and the risk of developing essential hypertension. METHODS: We conducted a case-control study in Burkina Faso, including 245 subjects with essential hypertension as case and 269 control subjects with normal blood pressure. Presence of the GSTT1 and GSTM1 was determined using conventional multiplex polymerase chain reaction followed by gel electrophoresis analysis. Biochemical parameters were measured using chemistry analyzer CYANExpert 130. RESULTS: Chi-squared test shows that GSTT1-null (OR = 1.82; p = 0.001) and GSTM1-active/GSTT1-null genotypes (OR = 2.33; p < 0.001) were significantly higher in cases than controls; the differences were not significant for GSTM1-null, GSTM1-null/GSTT1-active and GSTM1-null/GSTT1-null (p > 0.05). Multinomial logistic regression revealed that age ≥ 50 years, central obesity, family history of hypertension, obesity, alcohol intake and GSTT1 deletion were in decreasing order independent risk factors for essential hypertension. Analysis by gender, BMI and alcohol showed that association of GSTT1-null with risk of essential hypertension seems to be significant when BMI < 30 Kg/m(2), in non-smokers and in alcohol users (all OR ≥ 1.77; p ≤ 0.008). Concerning GSTT1, GSTM1 and cardiovascular risk markers levels in hypertensive group, we found that subjects with GSTT1-null genotype had higher waist circumference and higher HDL cholesterol level than those with GSTT1-active (all p < 0.005), subjects with GSTM1-null genotype had lower triglyceride than those with GSTM1-active (p = 0.02) and subjects with the double deletion GSTM1-null/GSTT1-null had higher body mass index, higher waist circumference and higher HDL cholesterol than those with GSTM1-active/GSTT1-active genotype (all p = 0.01). CONCLUSION: Our results confirm that GSTT1-null genotype is significantly associated with risk of developing essential hypertension in Burkinabe, especially when BMI < 30 Kg/m(2), in non-smokers and in alcohol users, and it showed that the double deletion GSTM1-null/GSTT1-null genotypes may influence body lipids repartition. BioMed Central 2020-03-19 /pmc/articles/PMC7081581/ /pubmed/32188413 http://dx.doi.org/10.1186/s12881-020-0990-9 Text en © The Author(s). 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Article Sombié, Herman Karim Sorgho, Abel Pegdwendé Kologo, Jonas Koudougou Ouattara, Abdoul Karim Yaméogo, Sakinata Yonli, Albert Théophane Djigma, Florencia Wendkuuni Tchelougou, Daméhan Somda, Dogfounianalo Kiendrébéogo, Isabelle Touwendpoulimdé Bado, Prosper Nagalo, Bolni Marius Nagabila, Youssoufou Adoko, Enagnon Tiémoko Herman Donald Zabsonré, Patrice Millogo, Hassanata Simporé, Jacques Glutathione S-transferase M1 and T1 genes deletion polymorphisms and risk of developing essential hypertension: a case-control study in Burkina Faso population (West Africa) |
title | Glutathione S-transferase M1 and T1 genes deletion polymorphisms and risk of developing essential hypertension: a case-control study in Burkina Faso population (West Africa) |
title_full | Glutathione S-transferase M1 and T1 genes deletion polymorphisms and risk of developing essential hypertension: a case-control study in Burkina Faso population (West Africa) |
title_fullStr | Glutathione S-transferase M1 and T1 genes deletion polymorphisms and risk of developing essential hypertension: a case-control study in Burkina Faso population (West Africa) |
title_full_unstemmed | Glutathione S-transferase M1 and T1 genes deletion polymorphisms and risk of developing essential hypertension: a case-control study in Burkina Faso population (West Africa) |
title_short | Glutathione S-transferase M1 and T1 genes deletion polymorphisms and risk of developing essential hypertension: a case-control study in Burkina Faso population (West Africa) |
title_sort | glutathione s-transferase m1 and t1 genes deletion polymorphisms and risk of developing essential hypertension: a case-control study in burkina faso population (west africa) |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7081581/ https://www.ncbi.nlm.nih.gov/pubmed/32188413 http://dx.doi.org/10.1186/s12881-020-0990-9 |
work_keys_str_mv | AT sombiehermankarim glutathionestransferasem1andt1genesdeletionpolymorphismsandriskofdevelopingessentialhypertensionacasecontrolstudyinburkinafasopopulationwestafrica AT sorghoabelpegdwende glutathionestransferasem1andt1genesdeletionpolymorphismsandriskofdevelopingessentialhypertensionacasecontrolstudyinburkinafasopopulationwestafrica AT kologojonaskoudougou glutathionestransferasem1andt1genesdeletionpolymorphismsandriskofdevelopingessentialhypertensionacasecontrolstudyinburkinafasopopulationwestafrica AT ouattaraabdoulkarim glutathionestransferasem1andt1genesdeletionpolymorphismsandriskofdevelopingessentialhypertensionacasecontrolstudyinburkinafasopopulationwestafrica AT yameogosakinata glutathionestransferasem1andt1genesdeletionpolymorphismsandriskofdevelopingessentialhypertensionacasecontrolstudyinburkinafasopopulationwestafrica AT yonlialberttheophane glutathionestransferasem1andt1genesdeletionpolymorphismsandriskofdevelopingessentialhypertensionacasecontrolstudyinburkinafasopopulationwestafrica AT djigmaflorenciawendkuuni glutathionestransferasem1andt1genesdeletionpolymorphismsandriskofdevelopingessentialhypertensionacasecontrolstudyinburkinafasopopulationwestafrica AT tchelougoudamehan glutathionestransferasem1andt1genesdeletionpolymorphismsandriskofdevelopingessentialhypertensionacasecontrolstudyinburkinafasopopulationwestafrica AT somdadogfounianalo glutathionestransferasem1andt1genesdeletionpolymorphismsandriskofdevelopingessentialhypertensionacasecontrolstudyinburkinafasopopulationwestafrica AT kiendrebeogoisabelletouwendpoulimde glutathionestransferasem1andt1genesdeletionpolymorphismsandriskofdevelopingessentialhypertensionacasecontrolstudyinburkinafasopopulationwestafrica AT badoprosper glutathionestransferasem1andt1genesdeletionpolymorphismsandriskofdevelopingessentialhypertensionacasecontrolstudyinburkinafasopopulationwestafrica AT nagalobolnimarius glutathionestransferasem1andt1genesdeletionpolymorphismsandriskofdevelopingessentialhypertensionacasecontrolstudyinburkinafasopopulationwestafrica AT nagabilayoussoufou glutathionestransferasem1andt1genesdeletionpolymorphismsandriskofdevelopingessentialhypertensionacasecontrolstudyinburkinafasopopulationwestafrica AT adokoenagnontiemokohermandonald glutathionestransferasem1andt1genesdeletionpolymorphismsandriskofdevelopingessentialhypertensionacasecontrolstudyinburkinafasopopulationwestafrica AT zabsonrepatrice glutathionestransferasem1andt1genesdeletionpolymorphismsandriskofdevelopingessentialhypertensionacasecontrolstudyinburkinafasopopulationwestafrica AT millogohassanata glutathionestransferasem1andt1genesdeletionpolymorphismsandriskofdevelopingessentialhypertensionacasecontrolstudyinburkinafasopopulationwestafrica AT simporejacques glutathionestransferasem1andt1genesdeletionpolymorphismsandriskofdevelopingessentialhypertensionacasecontrolstudyinburkinafasopopulationwestafrica |