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Comparison of leaf transcriptome in response to Rhizoctonia solani infection between resistant and susceptible rice cultivars

BACKGROUND: Sheath blight (SB), caused by Rhizoctonia solani, is a common rice disease worldwide. Currently, rice cultivars with robust resistance to R. solani are still lacking. To provide theoretic basis for molecular breeding of R. solani-resistant rice cultivars, the changes of transcriptome pro...

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Autores principales: Shi, Wei, Zhao, Shao-Lu, Liu, Kai, Sun, Yi-Biao, Ni, Zheng-Bin, Zhang, Gui-Yun, Tang, Hong-Sheng, Zhu, Jing-Wen, Wan, Bai-Jie, Sun, Hong-Qin, Dai, Jin-Ying, Sun, Ming-Fa, Yan, Guo-Hong, Wang, Ai-Min, Zhu, Guo-Yong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7081601/
https://www.ncbi.nlm.nih.gov/pubmed/32188400
http://dx.doi.org/10.1186/s12864-020-6645-6
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author Shi, Wei
Zhao, Shao-Lu
Liu, Kai
Sun, Yi-Biao
Ni, Zheng-Bin
Zhang, Gui-Yun
Tang, Hong-Sheng
Zhu, Jing-Wen
Wan, Bai-Jie
Sun, Hong-Qin
Dai, Jin-Ying
Sun, Ming-Fa
Yan, Guo-Hong
Wang, Ai-Min
Zhu, Guo-Yong
author_facet Shi, Wei
Zhao, Shao-Lu
Liu, Kai
Sun, Yi-Biao
Ni, Zheng-Bin
Zhang, Gui-Yun
Tang, Hong-Sheng
Zhu, Jing-Wen
Wan, Bai-Jie
Sun, Hong-Qin
Dai, Jin-Ying
Sun, Ming-Fa
Yan, Guo-Hong
Wang, Ai-Min
Zhu, Guo-Yong
author_sort Shi, Wei
collection PubMed
description BACKGROUND: Sheath blight (SB), caused by Rhizoctonia solani, is a common rice disease worldwide. Currently, rice cultivars with robust resistance to R. solani are still lacking. To provide theoretic basis for molecular breeding of R. solani-resistant rice cultivars, the changes of transcriptome profiles in response to R. solani infection were compared between a moderate resistant cultivar (Yanhui-888, YH) and a susceptible cultivar (Jingang-30, JG). RESULTS: In the present study, 3085 differentially express genes (DEGs) were detected between the infected leaves and the control in JG, with 2853 DEGs in YH. A total of 4091 unigenes were significantly upregulated in YH than in JG before infection, while 3192 were significantly upregulated after infection. Further analysis revealed that YH and JG showed similar molecular responses to R. solani infection, but the responses were earlier in JG than in YH. Expression levels of trans-cinnamate 4-monooxygenase (C4H), ethylene-insensitive protein 2 (EIN2), transcriptome factor WRKY33 and the KEGG pathway plant-pathogen interaction were significantly affected by R. solani infection. More importantly, these components were all over-represented in YH cultivar than in JG cultivar before and/or after infection. CONCLUSIONS: These genes possibly contribute to the higher resistance of YH to R. solani than JG and were potential target genes to molecularly breed R. solani-resistant rice cultivar.
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spelling pubmed-70816012020-03-23 Comparison of leaf transcriptome in response to Rhizoctonia solani infection between resistant and susceptible rice cultivars Shi, Wei Zhao, Shao-Lu Liu, Kai Sun, Yi-Biao Ni, Zheng-Bin Zhang, Gui-Yun Tang, Hong-Sheng Zhu, Jing-Wen Wan, Bai-Jie Sun, Hong-Qin Dai, Jin-Ying Sun, Ming-Fa Yan, Guo-Hong Wang, Ai-Min Zhu, Guo-Yong BMC Genomics Research Article BACKGROUND: Sheath blight (SB), caused by Rhizoctonia solani, is a common rice disease worldwide. Currently, rice cultivars with robust resistance to R. solani are still lacking. To provide theoretic basis for molecular breeding of R. solani-resistant rice cultivars, the changes of transcriptome profiles in response to R. solani infection were compared between a moderate resistant cultivar (Yanhui-888, YH) and a susceptible cultivar (Jingang-30, JG). RESULTS: In the present study, 3085 differentially express genes (DEGs) were detected between the infected leaves and the control in JG, with 2853 DEGs in YH. A total of 4091 unigenes were significantly upregulated in YH than in JG before infection, while 3192 were significantly upregulated after infection. Further analysis revealed that YH and JG showed similar molecular responses to R. solani infection, but the responses were earlier in JG than in YH. Expression levels of trans-cinnamate 4-monooxygenase (C4H), ethylene-insensitive protein 2 (EIN2), transcriptome factor WRKY33 and the KEGG pathway plant-pathogen interaction were significantly affected by R. solani infection. More importantly, these components were all over-represented in YH cultivar than in JG cultivar before and/or after infection. CONCLUSIONS: These genes possibly contribute to the higher resistance of YH to R. solani than JG and were potential target genes to molecularly breed R. solani-resistant rice cultivar. BioMed Central 2020-03-19 /pmc/articles/PMC7081601/ /pubmed/32188400 http://dx.doi.org/10.1186/s12864-020-6645-6 Text en © The Author(s). 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Shi, Wei
Zhao, Shao-Lu
Liu, Kai
Sun, Yi-Biao
Ni, Zheng-Bin
Zhang, Gui-Yun
Tang, Hong-Sheng
Zhu, Jing-Wen
Wan, Bai-Jie
Sun, Hong-Qin
Dai, Jin-Ying
Sun, Ming-Fa
Yan, Guo-Hong
Wang, Ai-Min
Zhu, Guo-Yong
Comparison of leaf transcriptome in response to Rhizoctonia solani infection between resistant and susceptible rice cultivars
title Comparison of leaf transcriptome in response to Rhizoctonia solani infection between resistant and susceptible rice cultivars
title_full Comparison of leaf transcriptome in response to Rhizoctonia solani infection between resistant and susceptible rice cultivars
title_fullStr Comparison of leaf transcriptome in response to Rhizoctonia solani infection between resistant and susceptible rice cultivars
title_full_unstemmed Comparison of leaf transcriptome in response to Rhizoctonia solani infection between resistant and susceptible rice cultivars
title_short Comparison of leaf transcriptome in response to Rhizoctonia solani infection between resistant and susceptible rice cultivars
title_sort comparison of leaf transcriptome in response to rhizoctonia solani infection between resistant and susceptible rice cultivars
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7081601/
https://www.ncbi.nlm.nih.gov/pubmed/32188400
http://dx.doi.org/10.1186/s12864-020-6645-6
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